PMID- 24993166
OWN - NLM
STAT- MEDLINE
DCOM- 20150910
LR  - 20140811
IS  - 1096-0023 (Electronic)
IS  - 1043-4666 (Linking)
VI  - 69
IP  - 2
DP  - 2014 Oct
TI  - TNF-alpha reduces g0s2 expression and stimulates lipolysis through PPAR-gamma inhibition 
      in 3T3-L1 adipocytes.
PG  - 196-205
LID - S1043-4666(14)00165-3 [pii]
LID - 10.1016/j.cyto.2014.06.005 [doi]
AB  - Tumor necrosis factor-alpha (TNF-alpha) is a multifunctional cytokine that acts as a 
      mediator of obesity-linked insulin resistance (IR). It is commonly accepted that 
      macrophage-derived TNF-alpha acts in a paracrine manner on adjacent adipocytes, 
      induces lipolysis, which contributes to obesity-linked hyperglycemia. Several 
      studies suggested that G0/G1 switch gene 2 (g0s2) was up-regulated during 
      adipogenesis, and its protein could be degraded in response to TNF-alpha stimulation. 
      The aim of the present work was to investigate the transcriptional regulation of 
      g0s2 by TNF-alpha stimulation. In this study, 3T3-L1 pre-adipocytes were 
      differentiated, and treated with TNF-alpha for 24h. The effects of TNF-alpha on lipolysis 
      and lipase expression were then examined. Our results revealed that TNF-alpha exerted 
      dose- and time-dependent lipolytic effects, which could be partially reversed by 
      overexpression of g0s2 and peroxisome proliferator-activated receptor-gamma (ppar-gamma). 
      In addition, TNF-alpha treatment significantly reduced the expression of adiponectin, 
      ppar-gamma, hormone-sensitive Lipase (hsl), adipose triglyceride lipase (atgl) as 
      well as ATGL co-factors. Interestingly, TNF-alpha significantly decreased adiponectin 
      and PPAR-gamma protein levels, while treatment with the proteasomal inhibitor MG-132 
      maintained PPAR-gamma levels. Degradation of PPAR-gamma almost completely abolished the 
      binding of PPAR-gamma to the g0s2 promoter in adipocytes treated with TNF-alpha. We 
      propose that proteasomal degradation of PPAR-gamma and the reduction of g0s2 content 
      are permissive for prolonged TNF-alpha induced lipolysis.
CI  - Copyright (c) 2014 Elsevier Ltd. All rights reserved.
FAU - Jin, Dan
AU  - Jin D
AD  - Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of 
      Ministry of Education, College of Life Science and Technology, Huazhong 
      Agricultural University, Wuhan 430070, PR China. Electronic address: 
      dan.king1983@gmail.com.
FAU - Sun, Jun
AU  - Sun J
AD  - Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of 
      Ministry of Education, College of Life Science and Technology, Huazhong 
      Agricultural University, Wuhan 430070, PR China.
FAU - Huang, Jing
AU  - Huang J
AD  - Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of 
      Ministry of Education, College of Life Science and Technology, Huazhong 
      Agricultural University, Wuhan 430070, PR China.
FAU - He, Yiduo
AU  - He Y
AD  - Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of 
      Ministry of Education, College of Life Science and Technology, Huazhong 
      Agricultural University, Wuhan 430070, PR China.
FAU - Yu, An
AU  - Yu A
AD  - Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of 
      Ministry of Education, College of Life Science and Technology, Huazhong 
      Agricultural University, Wuhan 430070, PR China.
FAU - Yu, Xiaoling
AU  - Yu X
AD  - Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of 
      Ministry of Education, College of Life Science and Technology, Huazhong 
      Agricultural University, Wuhan 430070, PR China.
FAU - Yang, Zaiqing
AU  - Yang Z
AD  - Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of 
      Ministry of Education, College of Life Science and Technology, Huazhong 
      Agricultural University, Wuhan 430070, PR China. Electronic address: 
      yangzq@mail.hzau.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140701
PL  - England
TA  - Cytokine
JT  - Cytokine
JID - 9005353
RN  - 0 (Adiponectin)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (G0S2 protein, mouse)
RN  - 0 (PPAR gamma)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 2.3.1.51 (1-Acylglycerol-3-Phosphate O-Acyltransferase)
RN  - EC 2.3.1.51 (Abhd5 protein, mouse)
RN  - EC 3.1.1.3 (Lipase)
RN  - EC 3.4.25.1 (Proteasome Endopeptidase Complex)
RN  - PDC6A3C0OX (Glycerol)
SB  - IM
MH  - 1-Acylglycerol-3-Phosphate O-Acyltransferase/metabolism
MH  - 3T3-L1 Cells
MH  - Adipocytes/drug effects/*metabolism
MH  - Adiponectin/metabolism
MH  - Animals
MH  - Cell Cycle Proteins/*genetics/metabolism
MH  - Cell Shape/drug effects
MH  - Down-Regulation/drug effects
MH  - Glycerol/metabolism
MH  - Lipase/metabolism
MH  - Lipolysis/*drug effects/genetics
MH  - Mice
MH  - PPAR gamma/*antagonists & inhibitors/metabolism
MH  - Promoter Regions, Genetic/genetics
MH  - Proteasome Endopeptidase Complex/drug effects/metabolism
MH  - Tumor Necrosis Factor-alpha/*pharmacology
OTO - NOTNLM
OT  - Adipocyte
OT  - G0S2
OT  - Lipolysis
OT  - PPAR-gamma
OT  - TNF-alpha
EDAT- 2014/07/06 06:00
MHDA- 2015/09/12 06:00
CRDT- 2014/07/05 06:00
PHST- 2013/08/12 00:00 [received]
PHST- 2014/05/22 00:00 [revised]
PHST- 2014/06/04 00:00 [accepted]
PHST- 2014/07/05 06:00 [entrez]
PHST- 2014/07/06 06:00 [pubmed]
PHST- 2015/09/12 06:00 [medline]
AID - S1043-4666(14)00165-3 [pii]
AID - 10.1016/j.cyto.2014.06.005 [doi]
PST - ppublish
SO  - Cytokine. 2014 Oct;69(2):196-205. doi: 10.1016/j.cyto.2014.06.005. Epub 2014 Jul 
      1.