PMID- 24993819
OWN - NLM
STAT- MEDLINE
DCOM- 20150309
LR  - 20211021
IS  - 1471-2407 (Electronic)
IS  - 1471-2407 (Linking)
VI  - 14
DP  - 2014 Jul 3
TI  - IL-1beta mediates MCP-1 induction by Wnt5a in gastric cancer cells.
PG  - 480
LID - 10.1186/1471-2407-14-480 [doi]
AB  - BACKGROUND: Both Wnt5a overexpression and macrophage infiltration have been 
      implicated in inflammation and cancer. The aim of this study is to reveal the 
      involvement of Wnt5a in macrophage recruitment in gastric cancer. METHODS: mRNA 
      expression in gastric cancer tissues and cells was investigated by real-time PCR. 
      Protein secretion by gastric cancer cells was determined by ELISA. PcDNA3.1-Wnt5a 
      expression vector and Wnt5a siRNA vector were used to overexpress and silence 
      Wnt5a expression in gastric cells, respectively. Macrophage migration was 
      analyzed by transwell, and macrophage cytoskeleton was stained with 
      FITC-phalloidin. RESULTS: Wnt5a was overexpressed in gastric cancer tissues, and 
      correlated with monocyte chemotactic protein 1 (MCP-1) and interleukin 1beta 
      (IL-1beta), respectively. In gastric cancer cells, Wnt5a induced MCP-1 expression, 
      which was mediated by IL-1beta. Conditioned medium from gastric cancer cells 
      transfected with Wnt5a stimulated macrophage chemotaxis and cytoskeletal changes 
      via MCP-1, which were suppressed by recombinant IL-1 receptor antagonist 
      (rIL-1Ra). CONCLUSIONS: These results suggest that Wnt5a is involved in 
      macrophage recruitment by upregulating MCP-1, and IL-1Ra may be used to inhibit 
      macrophage recruitment in gastric cancer.
FAU - Li, Shengjun
AU  - Li S
FAU - Wang, Wei
AU  - Wang W
FAU - Zhang, Ning
AU  - Zhang N
FAU - Ma, Tingxian
AU  - Ma T
FAU - Zhao, Chenghai
AU  - Zhao C
AD  - Department of Pathophysiology, College of Basic Medical Science, China Medical 
      University, Shenyang, China. zhaochenghai1@sina.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140703
PL  - England
TA  - BMC Cancer
JT  - BMC cancer
JID - 100967800
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (WNT5A protein, human)
RN  - 0 (Wnt Proteins)
RN  - 0 (Wnt-5a Protein)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Cell Line, Tumor
MH  - Chemokine CCL2/*genetics/metabolism
MH  - Chemotaxis, Leukocyte
MH  - Cytoskeleton/metabolism
MH  - Gene Expression
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Interleukin-1beta/*metabolism
MH  - Macrophages/immunology/metabolism
MH  - Mice
MH  - Proto-Oncogene Proteins/genetics/*metabolism
MH  - RNA, Messenger/genetics
MH  - Stomach Neoplasms/*genetics/immunology/*metabolism
MH  - Up-Regulation
MH  - Wnt Proteins/genetics/*metabolism
MH  - Wnt-5a Protein
PMC - PMC4086280
EDAT- 2014/07/06 06:00
MHDA- 2015/03/10 06:00
PMCR- 2014/07/03
CRDT- 2014/07/05 06:00
PHST- 2013/12/20 00:00 [received]
PHST- 2014/06/30 00:00 [accepted]
PHST- 2014/07/05 06:00 [entrez]
PHST- 2014/07/06 06:00 [pubmed]
PHST- 2015/03/10 06:00 [medline]
PHST- 2014/07/03 00:00 [pmc-release]
AID - 1471-2407-14-480 [pii]
AID - 10.1186/1471-2407-14-480 [doi]
PST - epublish
SO  - BMC Cancer. 2014 Jul 3;14:480. doi: 10.1186/1471-2407-14-480.