PMID- 24997907
OWN - NLM
STAT- MEDLINE
DCOM- 20141104
LR  - 20181202
IS  - 1879-1298 (Electronic)
IS  - 0045-6535 (Linking)
VI  - 111
DP  - 2014 Sep
TI  - Effects of enhanced denitrification on hydrodynamics and microbial community 
      structure in a soil column system.
PG  - 112-9
LID - S0045-6535(14)00382-8 [pii]
LID - 10.1016/j.chemosphere.2014.03.033 [doi]
AB  - Enhanced heterotrophic denitrification by adding glucose was investigated by 
      means of a soil column experiment which simulated the groundwater flow. The 
      carbon-to-nitrogen ratio was the main factor determining denitrification 
      potential under experimental conditions. The influence of stimulated 
      denitrification on the autochthonous microbial community was investigated by 
      quantitative PCR (qPCR), and denaturing gradient gel electrophoresis (DGGE). The 
      qPCR detection of the nosZ genes encoding nitrous oxide reductase, and the 
      comparison of the abundances of 16S rRNA genes revealed that the addition of 
      glucose enhanced denitrification leading to an increase in both the total 
      eubacteria and, in particular, in the ratio of denitrifying bacteria, which 
      represented the 21% of the total native eubacteria on the basis of nosZ/16S rRNA 
      gene ratio. Microbial community profiling by DGGE indicated that ribotypes 
      closely related to the genera Acidovorax and Hydrogenophaga (Comamonadaceae 
      family) became enriched in the soil column. The effects of biomass occurrence in 
      the column system on soil hydrodynamics, assessed by tracer studies, revealed a 
      reduction of porosity and a significant increase of dispersivity that could be 
      caused by the appearance of new functional microbial biomass in the aquifer 
      material under enhanced denitrifying conditions. The importance of investigating 
      the microbial growth in relation to the hydrodynamic effects, during enhanced 
      denitrification, has been revealed in the column system experiments associated 
      with the bioremediation. Combining microbial characterisation and hydrodynamic 
      data in a soil column system permits us to gain an insight to the limiting 
      factors of different stimulation strategies that can be applied in the field.
CI  - Copyright (c) 2014 Elsevier Ltd. All rights reserved.
FAU - Calderer, M
AU  - Calderer M
AD  - CTM Technological Centre, Environmental Technology Area, Placa de la Ciencia, No. 
      2, E-08243 Manresa, Spain. Electronic address: montse.calderer@ctm.com.es.
FAU - Marti, V
AU  - Marti V
AD  - CTM Technological Centre, Environmental Technology Area, Placa de la Ciencia, No. 
      2, E-08243 Manresa, Spain; Technical University of Catalonia, Department of 
      Chemical Engineering (UPC, ETSEIB), Av. Diagonal, No. 647, E-08028 Barcelona, 
      Spain.
FAU - de Pablo, J
AU  - de Pablo J
AD  - CTM Technological Centre, Environmental Technology Area, Placa de la Ciencia, No. 
      2, E-08243 Manresa, Spain; Technical University of Catalonia, Department of 
      Chemical Engineering (UPC, ETSEIB), Av. Diagonal, No. 647, E-08028 Barcelona, 
      Spain.
FAU - Guivernau, M
AU  - Guivernau M
AD  - IRTA, GIRO Joint Research Unit IRTA-UPC, Torre Marimon, E-08140 Caldes de 
      Montbui, Spain.
FAU - Prenafeta-Boldu, F X
AU  - Prenafeta-Boldu FX
AD  - IRTA, GIRO Joint Research Unit IRTA-UPC, Torre Marimon, E-08140 Caldes de 
      Montbui, Spain.
FAU - Vinas, M
AU  - Vinas M
AD  - IRTA, GIRO Joint Research Unit IRTA-UPC, Torre Marimon, E-08140 Caldes de 
      Montbui, Spain.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140419
PL  - England
TA  - Chemosphere
JT  - Chemosphere
JID - 0320657
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 7440-44-0 (Carbon)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.7.2.4 (nitrous oxide reductase)
RN  - N762921K75 (Nitrogen)
SB  - IM
MH  - *Biodiversity
MH  - Biomass
MH  - Carbon/chemistry
MH  - Comamonadaceae/genetics/isolation & purification
MH  - *Denitrification
MH  - Electrophoresis, Gel, Pulsed-Field
MH  - Groundwater/chemistry
MH  - *Hydrodynamics
MH  - Nitrogen/chemistry
MH  - Oxidoreductases/genetics/metabolism
MH  - RNA, Ribosomal, 16S/genetics
MH  - *Soil Microbiology
OTO - NOTNLM
OT  - 16S rRNA gene
OT  - DGGE
OT  - Enhanced denitrification
OT  - Hydrodynamics
OT  - Soil column
OT  - nosZ gene
EDAT- 2014/07/07 06:00
MHDA- 2014/11/05 06:00
CRDT- 2014/07/07 06:00
PHST- 2013/10/01 00:00 [received]
PHST- 2014/02/19 00:00 [revised]
PHST- 2014/03/01 00:00 [accepted]
PHST- 2014/07/07 06:00 [entrez]
PHST- 2014/07/07 06:00 [pubmed]
PHST- 2014/11/05 06:00 [medline]
AID - S0045-6535(14)00382-8 [pii]
AID - 10.1016/j.chemosphere.2014.03.033 [doi]
PST - ppublish
SO  - Chemosphere. 2014 Sep;111:112-9. doi: 10.1016/j.chemosphere.2014.03.033. Epub 
      2014 Apr 19.