PMID- 25001634
OWN - NLM
STAT- MEDLINE
DCOM- 20150527
LR  - 20211021
IS  - 1573-7330 (Electronic)
IS  - 1058-0468 (Print)
IS  - 1058-0468 (Linking)
VI  - 31
IP  - 9
DP  - 2014 Sep
TI  - Meiotic segregation and sperm DNA fragmentation in Tunisian men with dysplasia of 
      the fibrous sheath (DFS) associated with head abnormalities.
PG  - 1167-74
LID - 10.1007/s10815-014-0290-4 [doi]
AB  - PURPOSE: Dysplasia of the Fibrous Sheath (DFS) is a primitive flagellar pathology 
      for which a broad spectrum of ultrastructural flagellar abnormalities has been 
      described responsible for a severe to total asthenozoospermia. To this phenotype 
      other morphological abnormalities including cephalic and abnormalities in nuclear 
      structure can be associated that could compromise embryonic development in case 
      of use of Assisted Reproductive Technology (ART). The aim of this study was to 
      evaluate the level of DNA fragmentation and aneuploidy rate in ejaculated 
      spermatozoa of Tunisian men presented with DFS sperm defect associated to high 
      percentage of head abnormalities and to compare the results with those from 
      fertile men. METHODS: Sperm DNA fragmentation was evaluated by the terminal 
      desoxynucleotidyl transferase mediated deoxyuridine triphosphate biotin nick-end 
      labelling (TUNEL) assay. The study of meiotic segregation was performed by 
      Fluorescence in situ hybridization (FISH) for chromosomes X, Y and 18. RESULTS: 
      The mean DNA fragmentation index was significantly higher in patients compared to 
      the control group. FISH revealed a significantly higher incidence of sperm 
      aneuploidies compared with controls. All patients showed elevated frequencies of 
      sex chromosomes disomy, disomy 18 and diploidy. CONCLUSIONS: In some cases of 
      syndromic teratozoospermia due to sperm tail structural abnormalities, such as 
      DFS, other morphological cephalic abnormalities may be associated. In these cases 
      we have demonstrated impaired sperm nuclear quality which will affect the results 
      in ICSI. Hence the interest of a thorough study of the sperm nucleus in these 
      forms of infertility in order to predict the chances of success in ART.
FAU - Ghedir, H
AU  - Ghedir H
AD  - Laboratory of Cytogenetics, Molecular Genetics and Reproductive Biology, Farhat 
      Hached University Teaching Hospital, 4000, Sousse, Tunisia, 
      ghedir.houda@hotmail.fr.
FAU - Mehri, A
AU  - Mehri A
FAU - Mehdi, M
AU  - Mehdi M
FAU - Brahem, S
AU  - Brahem S
FAU - Saad, A
AU  - Saad A
FAU - Ibala-Romdhane, S
AU  - Ibala-Romdhane S
LA  - eng
PT  - Journal Article
DEP - 20140708
PL  - Netherlands
TA  - J Assist Reprod Genet
JT  - Journal of assisted reproduction and genetics
JID - 9206495
SB  - IM
MH  - Adult
MH  - Aneuploidy
MH  - Asthenozoospermia/*genetics/pathology
MH  - *Chromosome Segregation
MH  - *DNA Fragmentation
MH  - Humans
MH  - In Situ Nick-End Labeling
MH  - Male
MH  - Meiosis
MH  - Spermatozoa/*abnormalities/ultrastructure
MH  - Tunisia
PMC - PMC4156953
EDAT- 2014/07/09 06:00
MHDA- 2015/05/28 06:00
PMCR- 2015/09/01
CRDT- 2014/07/09 06:00
PHST- 2014/04/15 00:00 [received]
PHST- 2014/06/30 00:00 [accepted]
PHST- 2014/07/09 06:00 [entrez]
PHST- 2014/07/09 06:00 [pubmed]
PHST- 2015/05/28 06:00 [medline]
PHST- 2015/09/01 00:00 [pmc-release]
AID - 290 [pii]
AID - 10.1007/s10815-014-0290-4 [doi]
PST - ppublish
SO  - J Assist Reprod Genet. 2014 Sep;31(9):1167-74. doi: 10.1007/s10815-014-0290-4. 
      Epub 2014 Jul 8.