PMID- 25001930
OWN - NLM
STAT- MEDLINE
DCOM- 20140930
LR  - 20140708
IS  - 1007-8738 (Print)
IS  - 1007-8738 (Linking)
VI  - 30
IP  - 7
DP  - 2014 Jul
TI  - [Knock-down of CARMA1 in K562 cells inhibits the invasion and metastasis of K562 
      cells].
PG  - 691-5
AB  - OBJECTIVE: To study the effects of caspase recruitment domain membrane-associated 
      guanylate kinase protein 1 (CARMA1) knock-down using RNAi technology on cell 
      proliferation, colony forming, invasion and metastasis of the K562 cells. 
      METHODS: K562 cells with stably-silenced CARMA1 gene was constructed by 
      lentivirus-mediated RNAi technology. CARMA1 mRNA and protein levels were 
      respectively detected by reverse transcription PCR (RT-PCR) and Western blotting. 
      Cell proliferation was analyzed by trypan blue exclusion; colony forming was 
      detected by colony forming assay; invasion and metastasis in vitro were 
      determined with Transwell(R) assay with or without matrigel. Signaling pathway 
      molecules were detected by RT-PCR and Western blotting. RESULTS: Six cell lines 
      with knocked-down gene were successfully constructed. Of them, one was negative 
      control (K562/sh-eGFP), and the other five were CARMA1-silenced cells. 
      K562/shCARMA 1-93 showed the greatest inhibition of CARMA 1 gene and protein 
      expressions. Trypan blue exclusion and colony forming assay showed that the 
      ability of cell proliferation and colony forming were significantly suppressed in 
      K562/shCARMA 1-93 (P<0.01). Compared with blank control K562 and negative control 
      K562/sh-eGFP, the growth inhibition rates were respectively 29.3% and 28.6%; the 
      colony forming inhibition rates were respectively 37.6% and 34.1%; Transwell(R) 
      assay with or without matrigel showed that the number of K562/shCARMA1-93 which 
      passed through the membrane was obviously lower than that of the control group 
      (P<0.01). The expressions of nucleic transcription factor-kappaB (NF-kappaB) and early 
      growth response 1 (EGR1) were reduced following the inhibition of CARMA1. 
      CONCLUSION: CARMA1 gene knock-down had an impact on cell proliferation, colony 
      forming, invasion and metastasis of K562 cells, which is possibly related to the 
      inhibition of NF-kappaB and JNK/EGR-1 signaling pathways.
FAU - Liu, Fang
AU  - Liu F
AD  - Immuno-Oncology Laboratory, Fujian Provincial Cancer Hospital, Teaching Hospital, 
      Fujian Medical University; Fujian Provincial Key Laboratory of Translational 
      Cancer Medicine, Fuzhou 350014, China.
FAU - Wang, Lingyan
AU  - Wang L
AD  - Immuno-Oncology Laboratory, Fujian Provincial Cancer Hospital, Teaching Hospital, 
      Fujian Medical University; Fujian Provincial Key Laboratory of Translational 
      Cancer Medicine, Fuzhou 350014, China.
FAU - Li, Jieyu
AU  - Li J
AD  - Immuno-Oncology Laboratory, Fujian Provincial Cancer Hospital, Teaching Hospital, 
      Fujian Medical University; Fujian Provincial Key Laboratory of Translational 
      Cancer Medicine, Fuzhou 350014, China.
FAU - Chen, Shuping
AU  - Chen S
AD  - Immuno-Oncology Laboratory, Fujian Provincial Cancer Hospital, Teaching Hospital, 
      Fujian Medical University; Fujian Provincial Key Laboratory of Translational 
      Cancer Medicine, Fuzhou 350014, China.
FAU - Ye, Yunbin
AU  - Ye Y
AD  - Immuno-Oncology Laboratory, Fujian Provincial Cancer Hospital, Teaching Hospital, 
      Fujian Medical University; Fujian Provincial Key Laboratory of Translational 
      Cancer Medicine, Fuzhou 350014, China.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
JT  - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular 
      immunology
JID - 101139110
RN  - 0 (CARD Signaling Adaptor Proteins)
RN  - 0 (EGR1 protein, human)
RN  - 0 (Early Growth Response Protein 1)
RN  - 0 (Transcription Factor RelA)
RN  - EC 4.6.1.2 (CARD11 protein, human)
RN  - EC 4.6.1.2 (Guanylate Cyclase)
SB  - IM
MH  - Blotting, Western
MH  - CARD Signaling Adaptor Proteins/*genetics/metabolism
MH  - Cell Movement/*genetics
MH  - *Cell Proliferation
MH  - Early Growth Response Protein 1/genetics/metabolism
MH  - Guanylate Cyclase/*genetics/metabolism
MH  - HEK293 Cells
MH  - Humans
MH  - K562 Cells
MH  - Neoplasm Invasiveness
MH  - Neoplasm Metastasis
MH  - *RNA Interference
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Signal Transduction/genetics
MH  - Transcription Factor RelA/genetics/metabolism
EDAT- 2014/07/09 06:00
MHDA- 2014/10/01 06:00
CRDT- 2014/07/09 06:00
PHST- 2014/07/09 06:00 [entrez]
PHST- 2014/07/09 06:00 [pubmed]
PHST- 2014/10/01 06:00 [medline]
PST - ppublish
SO  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2014 Jul;30(7):691-5.