PMID- 25005117
OWN - NLM
STAT- MEDLINE
DCOM- 20141111
LR  - 20211021
IS  - 1872-8421 (Electronic)
IS  - 0165-5728 (Print)
IS  - 0165-5728 (Linking)
VI  - 274
IP  - 1-2
DP  - 2014 Sep 15
TI  - Astrocyte CCL2 sustains immune cell infiltration in chronic experimental 
      autoimmune encephalomyelitis.
PG  - 53-61
LID - S0165-5728(14)00180-5 [pii]
LID - 10.1016/j.jneuroim.2014.06.009 [doi]
AB  - Chemokine (C-C motif) ligand 2 (CCL2), initially identified as monocyte 
      chemoattractant protein-1 (MCP-1), recruits immune cells to the central nervous 
      system (CNS) during autoimmune inflammation. CCL2 can be expressed by multiple 
      cell types, but which cells are responsible for CCL2 function during acute and 
      chronic phases of autoimmune disease is not known. We determined the role of CCL2 
      in astrocytes in vivo during experimental autoimmune encephalomyelitis (EAE) by 
      using Cre-loxP gene deletion. Mice with a conditional gene deletion of CCL2 from 
      astrocytes had less severe EAE late in disease while having a similar incidence 
      and severity of disease at onset as compared to wild type (WT) control 
      littermates. EAE mice devoid of CCL2 in astrocytes had less macrophage and T cell 
      inflammation in the white matter of the spinal cord and less diffuse activation 
      of astrocytes and microglia in both white and gray matter as well as less axonal 
      loss and demyelination, compared to WT littermates. These findings demonstrate 
      that CCL2 in astrocytes plays an important role in the continued recruitment of 
      immune cells and activation of glial cells in the CNS during chronic EAE, thereby 
      suggesting a novel cell specific target for neuroprotective treatments of chronic 
      neuroinflammatory diseases.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Kim, Roy Y
AU  - Kim RY
AD  - Molecular Cellular and Integrative Physiology Interdepartmental Ph.D. Program, 
      University of California, Los Angeles; Multiple Sclerosis Program, Department of 
      Neurology, University of California, Los Angeles.
FAU - Hoffman, Alexandria S
AU  - Hoffman AS
AD  - Multiple Sclerosis Program, Department of Neurology, University of California, 
      Los Angeles.
FAU - Itoh, Noriko
AU  - Itoh N
AD  - Multiple Sclerosis Program, Department of Neurology, University of California, 
      Los Angeles.
FAU - Ao, Yan
AU  - Ao Y
AD  - Department of Neurobiology, University of California, Los Angeles.
FAU - Spence, Rory
AU  - Spence R
AD  - Multiple Sclerosis Program, Department of Neurology, University of California, 
      Los Angeles.
FAU - Sofroniew, Michael V
AU  - Sofroniew MV
AD  - Department of Neurobiology, University of California, Los Angeles.
FAU - Voskuhl, Rhonda R
AU  - Voskuhl RR
AD  - Multiple Sclerosis Program, Department of Neurology, University of California, 
      Los Angeles. Electronic address: rvoskuhl@ucla.edu.
LA  - eng
GR  - K24 NS062117/NS/NINDS NIH HHS/United States
GR  - T32 HD007228/HD/NICHD NIH HHS/United States
GR  - T32 HD07228-26/HD/NICHD NIH HHS/United States
GR  - K24NS052117/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20140624
PL  - Netherlands
TA  - J Neuroimmunol
JT  - Journal of neuroimmunology
JID - 8109498
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Chemokine CCL2)
SB  - IM
MH  - Animals
MH  - Astrocytes/*immunology
MH  - Chemokine CCL2/genetics/*immunology
MH  - Chronic Disease
MH  - Demyelinating Diseases/immunology
MH  - Encephalomyelitis, Autoimmune, Experimental/genetics/*immunology
MH  - Female
MH  - Macrophages/immunology
MH  - Male
MH  - Mice
MH  - Mice, Knockout
MH  - Microglia/immunology
MH  - Myelin Sheath/immunology
MH  - Spinal Cord/immunology
MH  - T-Lymphocytes/immunology
PMC - PMC4343306
MID - NIHMS665299
OTO - NOTNLM
OT  - Astrocytes
OT  - CCL2
OT  - EAE
OT  - Macrophage
OT  - Microglia
OT  - Multiple Sclerosis
OT  - Neuroprotection
OT  - T lymphocyte
EDAT- 2014/07/10 06:00
MHDA- 2014/11/12 06:00
PMCR- 2015/02/27
CRDT- 2014/07/10 06:00
PHST- 2014/02/19 00:00 [received]
PHST- 2014/06/09 00:00 [revised]
PHST- 2014/06/17 00:00 [accepted]
PHST- 2014/07/10 06:00 [entrez]
PHST- 2014/07/10 06:00 [pubmed]
PHST- 2014/11/12 06:00 [medline]
PHST- 2015/02/27 00:00 [pmc-release]
AID - S0165-5728(14)00180-5 [pii]
AID - 10.1016/j.jneuroim.2014.06.009 [doi]
PST - ppublish
SO  - J Neuroimmunol. 2014 Sep 15;274(1-2):53-61. doi: 10.1016/j.jneuroim.2014.06.009. 
      Epub 2014 Jun 24.