PMID- 2500579 OWN - NLM STAT- MEDLINE DCOM- 19890731 LR - 20121115 IS - 0026-2633 (Print) IS - 0026-2633 (Linking) VI - 57 IP - 230 DP - 1989 TI - Sporulation of bacterial species in the presence of metabisulphite. PG - 49-63 AB - The effect of metabisulphite on the sporulating ability of Bacillus subtilis E52 and B. cereus W18 was studied. Whereas metabisulphite concentrations of 500 and 600 micrograms ml-1 prevented sporulation of B. subtilis and B. cereus, respectively, lower concentrations caused reductions in their percentage sporulations. Glucose dehydrogenase (GDH) and alkaline phosphatase (ALP) activities of both sporulating bacteria were not detected at the stated metabisulphite concentrations. A relationship between the percentage sporulation of the bacteria and the activity of the enzymes in the presence of metabisulphite was exhibited. ALP activity may serve as an index of the effectiveness of the antisporulating activity of metabisulphite. Both enzymes are likely to be among the few targets for the antisporulating activity of metabisulphite. FAU - Oloyede, O B AU - Oloyede OB AD - Department of Biochemistry, University of Ilorin, Nigeria. FAU - Abalaka, J A AU - Abalaka JA LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - England TA - Microbios JT - Microbios JID - 0207257 RN - 0 (Sulfites) RN - 7992SO049K (metabisulfite) RN - EC 1.1.1.- (Glucose Dehydrogenases) RN - EC 3.1.3.1 (Alkaline Phosphatase) SB - IM MH - Alkaline Phosphatase/analysis MH - Bacillus subtilis/drug effects/enzymology/*physiology MH - Glucose Dehydrogenases/analysis MH - Spores, Bacterial MH - Sulfites/*pharmacology EDAT- 1989/01/01 00:00 MHDA- 1989/01/01 00:01 CRDT- 1989/01/01 00:00 PHST- 1989/01/01 00:00 [pubmed] PHST- 1989/01/01 00:01 [medline] PHST- 1989/01/01 00:00 [entrez] PST - ppublish SO - Microbios. 1989;57(230):49-63.