PMID- 25008928
OWN - NLM
STAT- MEDLINE
DCOM- 20150122
LR  - 20211021
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 88
IP  - 18
DP  - 2014 Sep
TI  - Detailed study of the interaction between human herpesvirus 6B glycoprotein 
      complex and its cellular receptor, human CD134.
PG  - 10875-82
LID - 10.1128/JVI.01447-14 [doi]
AB  - Recently, we identified a novel receptor, CD134, which interacts with the human 
      herpesvirus 6B (HHV-6B) glycoprotein (g)H/gL/gQ1/gQ2 complex and plays a key role 
      in the entry of HHV-6B into target cells. However, details of the interaction 
      between the HHV-6B gH/gL/gQ1/gQ2 complex and CD134 were unknown. In this study, 
      we identified a cysteine-rich domain (CRD), CDR2, of CD134 that is critical for 
      binding to the HHV-6B glycoprotein complex and HHV-6B infection. Furthermore, we 
      found that the expression of HHV-6B gQ1 and gQ2 subunits was sufficient for CD134 
      binding, which is different from the binding of human herpesvirus 6A (HHV-6A) to 
      its receptor, CD46. Finally, we identified a region in gQ1 critical for HHV-6B 
      gQ1 function. These results contribute much to our understanding of the 
      interaction between this ligand and receptor. IMPORTANCE: We identified the 
      domain in HHV-6B entry receptor CD134 and the components in the HHV-6B 
      gH/gL/gQ1/gQ2 complex required for ligand-receptor binding during HHV-6B 
      infection. Furthermore, we identified domains in gQ1 proteins of HHV-6A and -6B 
      and a key amino acid residue in HHV-6B gQ1 required for its function. These data 
      should be the basis for further investigation of ligand-receptor interaction in 
      the study of HHV-6A and -6B.
CI  - Copyright (c) 2014, American Society for Microbiology. All Rights Reserved.
FAU - Tang, Huamin
AU  - Tang H
AD  - Division of Clinical Virology, Kobe University Graduate School of Medicine, Kobe, 
      Japan.
FAU - Wang, Junjie
AU  - Wang J
AD  - Division of Clinical Virology, Kobe University Graduate School of Medicine, Kobe, 
      Japan.
FAU - Mahmoud, Nora F
AU  - Mahmoud NF
AD  - Division of Clinical Virology, Kobe University Graduate School of Medicine, Kobe, 
      Japan.
FAU - Mori, Yasuko
AU  - Mori Y
AD  - Division of Clinical Virology, Kobe University Graduate School of Medicine, Kobe, 
      Japan ymori@med.kobe-u.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140709
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Glycoproteins)
RN  - 0 (Receptors, OX40)
RN  - 0 (Viral Envelope Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Glycoproteins/chemistry/genetics/*metabolism
MH  - Herpesvirus 6, Human/chemistry/genetics/*metabolism
MH  - Humans
MH  - Molecular Sequence Data
MH  - Protein Binding
MH  - Protein Structure, Tertiary
MH  - Receptors, OX40/chemistry/genetics/*metabolism
MH  - Roseolovirus Infections/genetics/*metabolism/virology
MH  - Sequence Alignment
MH  - Viral Envelope Proteins/chemistry/genetics/*metabolism
PMC - PMC4178835
EDAT- 2014/07/11 06:00
MHDA- 2015/01/23 06:00
PMCR- 2015/03/01
CRDT- 2014/07/11 06:00
PHST- 2014/07/11 06:00 [entrez]
PHST- 2014/07/11 06:00 [pubmed]
PHST- 2015/01/23 06:00 [medline]
PHST- 2015/03/01 00:00 [pmc-release]
AID - JVI.01447-14 [pii]
AID - 01447-14 [pii]
AID - 10.1128/JVI.01447-14 [doi]
PST - ppublish
SO  - J Virol. 2014 Sep;88(18):10875-82. doi: 10.1128/JVI.01447-14. Epub 2014 Jul 9.