PMID- 25009705
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20140710
LR  - 20211021
IS  - 1976-9148 (Print)
IS  - 2005-4483 (Electronic)
IS  - 1976-9148 (Linking)
VI  - 22
IP  - 3
DP  - 2014 May
TI  - Luteolin Inhibits the Activity, Secretion and Gene Expression of MMP-3 in 
      Cultured Articular Chondrocytes and Production of MMP-3 in the Rat Knee.
PG  - 239-45
LID - 10.4062/biomolther.2014.020 [doi]
AB  - We investigated whether luteolin affects the gene expression, secretion and 
      activity of matrix metalloproteinase-3 (MMP-3) in primary cultured rabbit 
      articular chondrocytes, as well as production of MMP-3 in the rat knee to 
      evaluate the potential chondro-protective effects of luteolin. Rabbit articular 
      chondrocytes were cultured in a monolayer and IL-1beta-induced gene expression 
      levels of MMP-3, MMP-1, MMP-13, a disintegrin and metalloproteinase with 
      thrombospondin motifs-4 (ADAMTS-4), ADAMTS-5 and type II collagen were measured 
      by reverse transcription - polymerase chain reaction (RT-PCR). Effects of 
      luteolin on interleukin-1beta (IL-1beta)-induced secretion and enzyme activity of MMP-3 
      in rabbit articular chondrocytes were investigated by western blot analysis and 
      casein zymography, respectively. The effect of luteolin on MMP-3 protein 
      production was also examined in vivo. The results were as follows: (1) luteolin 
      inhibited the gene expression levels of MMP-3, MMP-1, MMP-13, ADAMTS-4 and 
      ADAMTS-5. However, it increased the gene expression level of collagen in rabbit 
      articular chondrocytes; (2) luteolin inhibited the secretion and activity of 
      MMP-3; (3) luteolin inhibited in vivo production of MMP-3 protein. These results 
      suggest that luteolin can regulate the gene expression, secretion and activity of 
      MMP-3, by directly acting on articular chondrocytes.
FAU - Kang, Bun-Jung
AU  - Kang BJ
AD  - Department of Orthopedic Surgery and Institute of Health Sciences, School of 
      Medicine and Hospital, Gyeongsang National University, Jinju 660-702.
FAU - Ryu, Jiho
AU  - Ryu J
AD  - Department of Pharmacology, School of Medicine, Chungnam National University, 
      Daejeon 301-740, Republic of Korea.
FAU - Lee, Choong Jae
AU  - Lee CJ
AD  - Department of Pharmacology, School of Medicine, Chungnam National University, 
      Daejeon 301-740, Republic of Korea.
FAU - Hwang, Sun-Chul
AU  - Hwang SC
AD  - Department of Orthopedic Surgery and Institute of Health Sciences, School of 
      Medicine and Hospital, Gyeongsang National University, Jinju 660-702.
LA  - eng
PT  - Journal Article
PL  - Korea (South)
TA  - Biomol Ther (Seoul)
JT  - Biomolecules & therapeutics
JID - 101472832
PMC - PMC4060084
OTO - NOTNLM
OT  - Chondrocyte
OT  - Luteolin
OT  - Osteoarthritis
EDAT- 2014/07/11 06:00
MHDA- 2014/07/11 06:01
PMCR- 2014/05/01
CRDT- 2014/07/11 06:00
PHST- 2014/02/20 00:00 [received]
PHST- 2014/04/01 00:00 [revised]
PHST- 2014/04/09 00:00 [accepted]
PHST- 2014/07/11 06:00 [entrez]
PHST- 2014/07/11 06:00 [pubmed]
PHST- 2014/07/11 06:01 [medline]
PHST- 2014/05/01 00:00 [pmc-release]
AID - bt-22-3-239 [pii]
AID - 10.4062/biomolther.2014.020 [doi]
PST - ppublish
SO  - Biomol Ther (Seoul). 2014 May;22(3):239-45. doi: 10.4062/biomolther.2014.020.