PMID- 25009824
OWN - NLM
STAT- MEDLINE
DCOM- 20150223
LR  - 20211021
IS  - 2314-7156 (Electronic)
IS  - 2314-8861 (Print)
IS  - 2314-7156 (Linking)
VI  - 2014
DP  - 2014
TI  - Attenuation and immunogenicity of a live high pathogenic PRRSV vaccine candidate 
      with a 32-amino acid deletion in the nsp2 protein.
PG  - 810523
LID - 10.1155/2014/810523 [doi]
LID - 810523
AB  - A porcine reproductive and respiratory syndrome virus (PRRSV) QY1 was serially 
      passed on Marc-145 cells. Virulence of different intermediate derivatives of QY1 
      (P5, P60, P80, and P100) were determined. The study found that QY1 had been 
      gradually attenuated during the in vitro process. Pathogenicity study showed that 
      pigs inoculated with QY1 P100 and P80 did not develop any significant PRRS clinic 
      symptoms. However, mild-to-moderate clinical signs and acute HP-PRRSV symptoms of 
      infection were observed in pigs inoculated with QY1 P60 and P5, respectively. 
      Furthermore, we determined the whole genome sequences of these four intermediate 
      viruses. The results showed that after 100 passages, compared to QY1 P5, a total 
      of 32 amino acid mutations were found. Moreover, there were one nucleotide 
      deletion and a unique 34-amino acid deletion found at 5'UTR and in nsp2 gene 
      during the attenuation process, respectively. Such deletions were genetically 
      stable in vivo. Following PRRSV experimental challenge, pigs inoculated with a 
      single dose of QY1 P100 developed no significant clinic symptoms and well 
      tolerated lethal challenge, while QY1 P80 group still developed mild fever in the 
      clinic trial after challenge. Thus, we concluded that QY1 P100 was a promising 
      and highly attenuated PRRSV vaccine candidate.
FAU - Lu, Wenhui
AU  - Lu W
AD  - College of Animal Science, South China Agricultural University, Tianhe District, 
      Wushan Road, Guangzhou, Guangdong 510642, China.
FAU - Sun, Baoli
AU  - Sun B
AD  - College of Animal Science, South China Agricultural University, Tianhe District, 
      Wushan Road, Guangzhou, Guangdong 510642, China.
FAU - Mo, Jianyue
AU  - Mo J
AD  - College of Animal Science, South China Agricultural University, Tianhe District, 
      Wushan Road, Guangzhou, Guangdong 510642, China.
FAU - Zeng, Xiduo
AU  - Zeng X
AUID- ORCID: 0000-0001-5964-8213
AD  - College of Animal Science, South China Agricultural University, Tianhe District, 
      Wushan Road, Guangzhou, Guangdong 510642, China.
FAU - Zhang, Guanqun
AU  - Zhang G
AD  - Guangdong Wen's Foodstuff Group Co., Ltd., Yanjiang Street, Xinxing, Guangdong 
      527400, China.
FAU - Wang, Lianxiang
AU  - Wang L
AD  - Guangdong Wen's Foodstuff Group Co., Ltd., Yanjiang Street, Xinxing, Guangdong 
      527400, China.
FAU - Zhou, Qingfeng
AU  - Zhou Q
AD  - Guangdong Wen's Foodstuff Group Co., Ltd., Yanjiang Street, Xinxing, Guangdong 
      527400, China.
FAU - Zhu, Ling
AU  - Zhu L
AD  - College of Animal Science, South China Agricultural University, Tianhe District, 
      Wushan Road, Guangzhou, Guangdong 510642, China.
FAU - Li, Zhili
AU  - Li Z
AD  - College of Veterinary Medicine, South China Agricultural University, Tianhe 
      District, Wushan Road, Guangzhou, Guangdong 510642, China.
FAU - Xie, Qingmei
AU  - Xie Q
AD  - College of Animal Science, South China Agricultural University, Tianhe District, 
      Wushan Road, Guangzhou, Guangdong 510642, China.
FAU - Bi, Yinzuo
AU  - Bi Y
AD  - College of Animal Science, South China Agricultural University, Tianhe District, 
      Wushan Road, Guangzhou, Guangdong 510642, China ; Guangdong Wen's Foodstuff Group 
      Co., Ltd., Yanjiang Street, Xinxing, Guangdong 527400, China.
FAU - Ma, Jingyun
AU  - Ma J
AD  - College of Animal Science, South China Agricultural University, Tianhe District, 
      Wushan Road, Guangzhou, Guangdong 510642, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140609
PL  - Egypt
TA  - J Immunol Res
JT  - Journal of immunology research
JID - 101627166
RN  - 0 (Antibodies, Viral)
RN  - 0 (Vaccines, Attenuated)
RN  - 0 (Viral Nonstructural Proteins)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Amino Acid Substitution
MH  - Animals
MH  - Antibodies, Viral/immunology
MH  - Biopsy
MH  - Lung/immunology/pathology/virology
MH  - Mutation
MH  - Neutralization Tests
MH  - Porcine Reproductive and Respiratory Syndrome/diagnosis/*immunology/virology
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/*immunology
MH  - *Sequence Deletion
MH  - Swine
MH  - Vaccines, Attenuated/genetics/*immunology
MH  - Viral Nonstructural Proteins/*genetics/*immunology
MH  - Viral Vaccines/genetics/*immunology
MH  - Viremia
PMC - PMC4070328
EDAT- 2014/07/11 06:00
MHDA- 2015/02/24 06:00
PMCR- 2014/06/09
CRDT- 2014/07/11 06:00
PHST- 2013/12/17 00:00 [received]
PHST- 2014/05/04 00:00 [revised]
PHST- 2014/05/07 00:00 [accepted]
PHST- 2014/07/11 06:00 [entrez]
PHST- 2014/07/11 06:00 [pubmed]
PHST- 2015/02/24 06:00 [medline]
PHST- 2014/06/09 00:00 [pmc-release]
AID - 10.1155/2014/810523 [doi]
PST - ppublish
SO  - J Immunol Res. 2014;2014:810523. doi: 10.1155/2014/810523. Epub 2014 Jun 9.