PMID- 25010201
OWN - NLM
STAT- MEDLINE
DCOM- 20150819
LR  - 20211021
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Print)
IS  - 0003-2700 (Linking)
VI  - 86
IP  - 15
DP  - 2014 Aug 5
TI  - Reagentless, ratiometric electrochemical DNA sensors with improved robustness and 
      reproducibility.
PG  - 8010-6
LID - 10.1021/ac5025254 [doi]
AB  - To make the electrochemical DNA sensors (E-sensor) more robust and reproducible, 
      we have now for the first time adapted the techniques of ratiometric analyses to 
      the field of E-sensors. We did this via the simple expedient way of 
      simultaneously using two redox probes: Methylene blue as the reporter of the 
      conformational change, and ferrocene as an internal control. During the 
      conformational transduction, only the distance between the signal probe and the 
      electrode surface undergoes an appreciable change, while the distance between the 
      control probe and the electrode remains relatively constant. This special design 
      has allowed very reliable target recognition, as illustrated in this report using 
      a human T-lymphotropic virus type I gene fragment. The standard deviation between 
      measurements obtained using different electrodes was an order of magnitude less 
      than that obtained using a classic E-sensor, which we prepared as a control. A 
      limit of detection of 25.1 pM was obtained with our new system, with a single 
      mismatch discrimination factor of 2.33 likewise being observed. Additionally, 
      this concept had general applicability, and preliminary data of a "Signal-On" 
      ratiometric E-sensor are also provided. Taken in concert, these results serve to 
      validate the utility of what we believe will emerge as an easily generalized 
      approach to oligonucleotide recognition and sensing.
FAU - Du, Yan
AU  - Du Y
AD  - Institute for Cellular and Molecular Biology, Center for Systems and Synthetic 
      Biology, and Department of Chemistry, The University of Texas at Austin , Austin, 
      Texas 78712, United States.
FAU - Lim, Byung Joon
AU  - Lim BJ
FAU - Li, Bingling
AU  - Li B
FAU - Jiang, Yu Sherry
AU  - Jiang YS
FAU - Sessler, Jonathan L
AU  - Sessler JL
FAU - Ellington, Andrew D
AU  - Ellington AD
LA  - eng
GR  - R01 CA068682/CA/NCI NIH HHS/United States
GR  - CA 68682/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20140718
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (DNA Probes)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Base Sequence
MH  - DNA/*chemistry
MH  - DNA Probes
MH  - Electrochemical Techniques/*instrumentation
MH  - Genes, Viral
MH  - Human T-lymphotropic virus 1/genetics
MH  - Reproducibility of Results
PMC - PMC4372097
EDAT- 2014/07/11 06:00
MHDA- 2015/08/20 06:00
PMCR- 2015/07/10
CRDT- 2014/07/11 06:00
PHST- 2014/07/11 06:00 [entrez]
PHST- 2014/07/11 06:00 [pubmed]
PHST- 2015/08/20 06:00 [medline]
PHST- 2015/07/10 00:00 [pmc-release]
AID - 10.1021/ac5025254 [doi]
PST - ppublish
SO  - Anal Chem. 2014 Aug 5;86(15):8010-6. doi: 10.1021/ac5025254. Epub 2014 Jul 18.