PMID- 25010399
OWN - NLM
STAT- MEDLINE
DCOM- 20150608
LR  - 20140915
IS  - 1873-7544 (Electronic)
IS  - 0306-4522 (Linking)
VI  - 277
DP  - 2014 Sep 26
TI  - Increases in mature brain-derived neurotrophic factor protein in the frontal 
      cortex and basal forebrain during chronic sleep restriction in rats: possible 
      role in initiating allostatic adaptation.
PG  - 174-83
LID - S0306-4522(14)00552-1 [pii]
LID - 10.1016/j.neuroscience.2014.06.067 [doi]
AB  - Chronic sleep restriction (CSR) has various negative consequences on cognitive 
      performance and health. Using a rat model of CSR that uses alternating cycles of 
      3h of sleep deprivation (using slowly rotating activity wheels) and 1h of sleep 
      opportunity continuously for 4 days ('3/1' protocol), we previously observed not 
      only homeostatic but also allostatic (adaptive) sleep responses to CSR. In 
      particular, non-rapid eye movement sleep (NREMS) electroencephalogram (EEG) delta 
      power, an index of sleep intensity, increased initially and then declined 
      gradually during CSR, with no rebound during a 2-day recovery period. To study 
      underlying mechanisms of these allostatic responses, we examined the levels of 
      brain-derived neurotrophic factor (BDNF), which is known to regulate NREMS EEG 
      delta activity, during the same CSR protocol. Mature BDNF protein levels were 
      measured in the frontal cortex and basal forebrain, two brain regions involved in 
      sleep and EEG regulation, and the hippocampus, using Western blot analysis. Adult 
      male Wistar rats were housed in motorized activity wheels, and underwent the 3/1 
      CSR protocol for 27 h, for 99 h, or for 99 h followed by 24h of recovery. 
      Additional rats were housed in either locked wheels (locked wheel controls 
      [LWCs]) or unlocked wheels that rats could rotate freely (wheel-running controls 
      [WRCs]). BDNF levels did not differ between WRC and LWC groups. BDNF levels were 
      increased, compared to the control levels, in all three brain regions after 27 h, 
      and were increased less strongly after 99 h, of CSR. After 24h of recovery, BDNF 
      levels were at the control levels. This time course of BDNF levels parallels the 
      previously reported changes in NREMS delta power during the same CSR protocol. 
      Changes in BDNF protein levels in the cortex and basal forebrain may be part of 
      the molecular mechanisms underlying allostatic sleep responses to CSR.
CI  - Copyright (c) 2014 IBRO. Published by Elsevier Ltd. All rights reserved.
FAU - Wallingford, J K
AU  - Wallingford JK
AD  - Department of Medical Neuroscience, Dalhousie University, Halifax, Nova Scotia, 
      Canada.
FAU - Deurveilher, S
AU  - Deurveilher S
AD  - Department of Medical Neuroscience, Dalhousie University, Halifax, Nova Scotia, 
      Canada.
FAU - Currie, R W
AU  - Currie RW
AD  - Department of Medical Neuroscience, Dalhousie University, Halifax, Nova Scotia, 
      Canada.
FAU - Fawcett, J P
AU  - Fawcett JP
AD  - Department of Pharmacology, Dalhousie University, Halifax, Nova Scotia, Canada; 
      Department of Surgery, Dalhousie University, Halifax, Nova Scotia, Canada.
FAU - Semba, K
AU  - Semba K
AD  - Department of Medical Neuroscience, Dalhousie University, Halifax, Nova Scotia, 
      Canada; Department of Psychology & Neuroscience, Dalhousie University, Halifax, 
      Nova Scotia, Canada; Department of Psychiatry, Dalhousie University, Halifax, 
      Nova Scotia, Canada. Electronic address: semba@dal.ca.
LA  - eng
GR  - MOP-259183/Canadian Institutes of Health Research/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140708
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Brain-Derived Neurotrophic Factor)
SB  - IM
MH  - Adaptation, Physiological/*physiology
MH  - Adrenal Glands/physiology
MH  - Animals
MH  - Basal Forebrain/*metabolism
MH  - Blotting, Western
MH  - Body Weight
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Chronic Disease
MH  - Frontal Lobe/*metabolism
MH  - Hippocampus/*metabolism
MH  - Male
MH  - Motor Activity/physiology
MH  - Organ Size
MH  - Random Allocation
MH  - Rats, Wistar
MH  - Sleep Deprivation/*metabolism
OTO - NOTNLM
OT  - Western blot
OT  - allostasis
OT  - basal forebrain
OT  - frontal cortex
OT  - hippocampus
OT  - sleep deprivation
EDAT- 2014/07/11 06:00
MHDA- 2015/06/09 06:00
CRDT- 2014/07/11 06:00
PHST- 2014/04/18 00:00 [received]
PHST- 2014/06/27 00:00 [revised]
PHST- 2014/06/27 00:00 [accepted]
PHST- 2014/07/11 06:00 [entrez]
PHST- 2014/07/11 06:00 [pubmed]
PHST- 2015/06/09 06:00 [medline]
AID - S0306-4522(14)00552-1 [pii]
AID - 10.1016/j.neuroscience.2014.06.067 [doi]
PST - ppublish
SO  - Neuroscience. 2014 Sep 26;277:174-83. doi: 10.1016/j.neuroscience.2014.06.067. 
      Epub 2014 Jul 8.