PMID- 2501120
OWN - NLM
STAT- MEDLINE
DCOM- 19890818
LR  - 20190813
IS  - 0303-7207 (Print)
IS  - 0303-7207 (Linking)
VI  - 61
IP  - 1
DP  - 1989 Jan
TI  - Tumor necrosis factor-alpha regulates mRNA for urokinase-type plasminogen 
      activator and type-1 plasminogen activator inhibitor in human neoplastic cell 
      lines.
PG  - 87-96
AB  - Tumor necrosis factor-alpha (TNF-alpha) was found to induce type-1 plasminogen 
      activator inhibitor (PAI-1) antigen in the human fibrosarcoma cell line HT-1080, 
      and PAI-1 and urokinase-type plasminogen activator (u-PA) antigens in the human 
      carcinoma cell line T-CAR1; tissue-type plasminogen activator (t-PA) antigen was 
      not affected or slightly decreased. The effects in HT-1080 and T-CAR1 cells were 
      preceded by increases in the cellular levels of the corresponding mRNAs. 
      Cycloheximide caused an increase of PAI-1 mRNA in T-CAR1 cells, but not in 
      HT-1080 cells; during this increase the relative abundance of the two PAI-1 mRNA 
      species, of 2.3 kb and 3.4 kb, respectively, changed strongly in favor of the 
      longer transcript. We conclude that TNF-alpha may affect proteolytic activity in 
      the microenvironment of cells in malignant tumors by affecting gene expression of 
      u-PA and PAI-1.
FAU - Georg, B
AU  - Georg B
AD  - Institute of Biochemistry C, University of Copenhagen, Denmark.
FAU - Helseth, E
AU  - Helseth E
FAU - Lund, L R
AU  - Lund LR
FAU - Skandsen, T
AU  - Skandsen T
FAU - Riccio, A
AU  - Riccio A
FAU - Dano, K
AU  - Dano K
FAU - Unsgaard, G
AU  - Unsgaard G
FAU - Andreasen, P A
AU  - Andreasen PA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Mol Cell Endocrinol
JT  - Molecular and cellular endocrinology
JID - 7500844
RN  - 0 (Glycoproteins)
RN  - 0 (Plasminogen Inactivators)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 98600C0908 (Cycloheximide)
RN  - EC 3.4.21.- (Plasminogen Activators)
RN  - EC 3.4.21.68 (Tissue Plasminogen Activator)
RN  - EC 3.4.21.73 (Urokinase-Type Plasminogen Activator)
SB  - IM
MH  - Cycloheximide/pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Gene Expression Regulation
MH  - Glycoproteins/genetics/*metabolism
MH  - Humans
MH  - Hydrolysis
MH  - Plasminogen Activators/genetics/*metabolism
MH  - Plasminogen Inactivators
MH  - RNA, Messenger/genetics/*metabolism
MH  - Tissue Plasminogen Activator/analysis/genetics/metabolism
MH  - Transcription, Genetic
MH  - Tumor Cells, Cultured
MH  - Tumor Necrosis Factor-alpha/*pharmacology
MH  - Urokinase-Type Plasminogen Activator/genetics/*metabolism
EDAT- 1989/01/01 00:00
MHDA- 1989/01/01 00:01
CRDT- 1989/01/01 00:00
PHST- 1989/01/01 00:00 [pubmed]
PHST- 1989/01/01 00:01 [medline]
PHST- 1989/01/01 00:00 [entrez]
AID - 10.1016/0303-7207(89)90192-5 [doi]
PST - ppublish
SO  - Mol Cell Endocrinol. 1989 Jan;61(1):87-96. doi: 10.1016/0303-7207(89)90192-5.