PMID- 25011965
OWN - NLM
STAT- MEDLINE
DCOM- 20150908
LR  - 20181202
IS  - 1672-7347 (Print)
IS  - 1672-7347 (Linking)
VI  - 39
IP  - 6
DP  - 2014 Jun
TI  - [Detecting chromosomal aberrations in myelodysplastic syndrome with fluorescence 
      in situ hybridization and conventional cytogenetic analysis].
PG  - 605-11
LID - 10.11817/j.issn.1672-7347.2014.06.010 [doi]
AB  - OBJECTIVE: To detect chromosomal abnormalities in myelodysplastic syndrome (MDS) 
      patients by fluorescence in situ hybridization (FISH) and conventional 
      cytogenetic analysis (CCA). METHODS: FISH and CCA were performed in 100 patients 
      who were diagnosed with MDS by conventional detection of bone marrow smear and 
      bone marrow biopsy, and were followed up. RESULTS: Forty-eight (48%) patients 
      showed chromosomal abnormalities. The positive rate of -5/5q-, 20q-, +8, -7/7q-, 
      and -Y was 16%, 15%, 12%, 11%, and 5%, respectively, and that of CCA was 11%. The 
      positive rate of molecular genetics abnormalities detected by FISH was obviously 
      higher than that of CCA (P<0.01) and the combination of FISH and CCA increased 
      the detection rate to 49%. The follow-up showed that the prognosis of patients 
      with normal FISH results was significantly better than the abnormal ones. A 
      correlation between complex karyotypes and poor prognosis was observed. 
      Abnormality of -7/7q- was found closely correlated with the higher risk of acute 
      leukemia and death. CONCLUSION: Chromosomal abnormalities have been found in 49 
      MDS patients. Common chromosomal abnormalities in MDS patients include -5/5q-, 
      20q-, +8 and -7/7q-. FISH combined with CCA can improve the detection rate of 
      chromosomal aberrations in MDS. FISH is more sensitive than CCA for detection and 
      can be used as an important basis for prognostic assessment for MDS.
FAU - Cao, Pengfei
AU  - Cao P
AD  - Department of Hematology, Xiangya Hospital, Central South University, Changsha 
      410008; Cancer Research Institute, Central South University, Changsha 
      410078,China.
FAU - Li, Yuan
AU  - Li Y
FAU - Li, Xiaolin
AU  - Li X
FAU - Zhang, Guoping
AU  - Zhang G
FAU - Chen, Fangping
AU  - Chen F
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhong Nan Da Xue Xue Bao Yi Xue Ban
JT  - Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. 
      Medical sciences
JID - 101230586
SB  - IM
MH  - *Chromosome Aberrations
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Myelodysplastic Syndromes/diagnosis/*genetics
MH  - Prognosis
EDAT- 2014/07/12 06:00
MHDA- 2015/09/09 06:00
CRDT- 2014/07/12 06:00
PHST- 2014/07/12 06:00 [entrez]
PHST- 2014/07/12 06:00 [pubmed]
PHST- 2015/09/09 06:00 [medline]
AID - 10.11817/j.issn.1672-7347.2014.06.010 [doi]
PST - ppublish
SO  - Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2014 Jun;39(6):605-11. doi: 
      10.11817/j.issn.1672-7347.2014.06.010.