PMID- 25012471
OWN - NLM
STAT- MEDLINE
DCOM- 20150330
LR  - 20211021
IS  - 1471-2369 (Electronic)
IS  - 1471-2369 (Linking)
VI  - 15
DP  - 2014 Jul 9
TI  - Transplantation of endothelial progenitor cells in treating rats with IgA 
      nephropathy.
PG  - 110
LID - 10.1186/1471-2369-15-110 [doi]
AB  - BACKGROUND: Therapeutic options in IgAN are still limited. The aim of this study 
      is to explore the feasibility of using endothelial progenitor cell to treat IgAN 
      in rat model. METHODS: Rat bone marrow mononuclear cells (BM-MNCs) obtained with 
      density gradient centrifugation were cultured in vitro, and induced into 
      endothelial progenitor cells (EPCs). EPCs were identified by surface marker CD34, 
      CD133 and VEGFR2 (FLK-1) and by Dil-Ac-LDL/FITC-UEA-1 double staining. EPCs were 
      labeled with PKH26 prior to transplantation. Rat model of IgAN was established by 
      oral administration of bovine serum albumin together with lipopolysaccharide via 
      the caudal vein and subcutaneous injection of CCL4. Kidney paraffin sections were 
      stained by H&E and PAS. Immunofluorescence was used to assess IgA deposition in 
      the glomeruli. Peritubular capillary (PTC) density was determined by CD31 
      staining. Monocyte chemoattrant protein-1 (MCP-1), hypoxia-inducible factor-1alpha 
      (HIF-1alpha) and CD105 were also measured by immunohistochemistry, western blotting 
      and real-time fluorescent quantitative PCR. RESULTS: The transplanted BM-EPCs 
      were successfully located in IgAN rat kidney. After transplantation, Urinary red 
      blood cell, urine protein, BUN, Scr and IgA serum level were significantly 
      decreased, so were the areas of glomerular extracellular matrix and the IgA 
      deposition in the glomeruli. In addition, PTC density was elevated. And the 
      expression levels of HIF-1alpha and MCP-1 were significantly down-regulated, while 
      the expression of CD105 was up-regulated. All these changes were not observed in 
      control groups. CONCLUSION: The BM-EPCs transplantation significantly decreases 
      the expansion of glomerular extracellular matrix and the deposition of IgA in the 
      glomeruli; lowers the expression of inflammatory factors; increases PTC density; 
      improves ischemic-induced renal tissue hypoxia, all of which improves the renal 
      function and slows the progress of IgA nephropathy.
FAU - Guo, Wei
AU  - Guo W
FAU - Feng, Jiang-Min
AU  - Feng JM
AD  - Department of Nephrology, The First Affiliated Hospital of China Medical 
      University, Shenyang 110001, China. fengjiangm@163.com.
FAU - Yao, Li
AU  - Yao L
FAU - Sun, Li
AU  - Sun L
FAU - Zhu, Guang-Qing
AU  - Zhu GQ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140709
PL  - England
TA  - BMC Nephrol
JT  - BMC nephrology
JID - 100967793
SB  - IM
MH  - Animals
MH  - Bone Marrow Transplantation/*methods
MH  - Cells, Cultured
MH  - Endothelial Progenitor Cells/physiology/*transplantation
MH  - Female
MH  - Glomerulonephritis, IGA/metabolism/*pathology/*therapy
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Treatment Outcome
PMC - PMC4109798
EDAT- 2014/07/12 06:00
MHDA- 2015/03/31 06:00
PMCR- 2014/07/09
CRDT- 2014/07/12 06:00
PHST- 2013/10/28 00:00 [received]
PHST- 2014/07/02 00:00 [accepted]
PHST- 2014/07/12 06:00 [entrez]
PHST- 2014/07/12 06:00 [pubmed]
PHST- 2015/03/31 06:00 [medline]
PHST- 2014/07/09 00:00 [pmc-release]
AID - 1471-2369-15-110 [pii]
AID - 10.1186/1471-2369-15-110 [doi]
PST - epublish
SO  - BMC Nephrol. 2014 Jul 9;15:110. doi: 10.1186/1471-2369-15-110.