PMID- 25014768
OWN - NLM
STAT- MEDLINE
DCOM- 20150522
LR  - 20140827
IS  - 1742-2051 (Electronic)
IS  - 1742-2051 (Linking)
VI  - 10
IP  - 10
DP  - 2014 Oct
TI  - Vitreous proteomic analysis of idiopathic epiretinal membranes.
PG  - 2558-66
LID - 10.1039/c4mb00240g [doi]
AB  - To understand the molecular mechanisms of idiopathic epiretinal membranes 
      (iERMs), the vitreous proteomes of patients with iERMs were investigated. The 
      vitreous proteome in patients with iERMs (n = 8) and donor samples (n = 8) was 
      analysed using reversed phase high-performance liquid chromatography (RP-HPLC) 
      coupled with electrospray ionization tandem mass spectrometry (ESI-MS/MS) and 
      GeneGo Metacore. This research followed the tenets of the Declaration of 
      Helsinki for the use of human subjects. In this current study, 226 significant 
      changes in protein abundance (abundance ratio >2, p < 0.01) were identified in 
      the vitreous proteome of iERM patients compared to normal control vitreous, 
      including 122 proteins that were present at lower levels and 104 proteins that 
      were present at higher levels. In the iERM vitreous samples, complement 
      components, inflammation-related proteins and matrix metalloproteinase were 
      present at higher levels, while normal cytoskeleton proteins were present at 
      lower levels. The top GeneGo pathway was "immune response", the top process 
      network was "inflammation", and the top KEGG pathway was "coagulation cascades". 
      The essential 2-node proteins of the network were estrogen receptor 1 (ESR1) and 
      p300. Among those found at higher levels, ubiquitin-conjugating enzyme E2O 
      (UBE2O) and complement C4A (C4A) were the most abundant proteins, and could be 
      detected in each of the iERM vitreous samples. It can be concluded that iERMs are 
      a complicated pathological process involving inflammation, immune response, and 
      cytoskeleton remolding. UBE2O and C4A may be candidate biomarkers for iERMs.
FAU - Yu, Jing
AU  - Yu J
AD  - Department of Ophthalmology, Shanghai Tenth People's Hospital, Tongji University 
      School of Medicine, Shanghai 200072, P. R. China. dryujing@aliyun.com.
FAU - Feng, Le
AU  - Feng L
FAU - Wu, Yan
AU  - Wu Y
FAU - Wang, Hao
AU  - Wang H
FAU - Ba, Jun
AU  - Ba J
FAU - Zhu, Wei
AU  - Zhu W
FAU - Xie, Chunlei
AU  - Xie C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Mol Biosyst
JT  - Molecular bioSystems
JID - 101251620
RN  - 0 (Proteome)
SB  - IM
MH  - Aged
MH  - Computational Biology
MH  - Databases, Protein
MH  - Epiretinal Membrane/*metabolism
MH  - Female
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Protein Interaction Maps
MH  - *Proteome
MH  - *Proteomics/methods
MH  - Reproducibility of Results
MH  - Signal Transduction
MH  - Vitreous Body/*metabolism
EDAT- 2014/07/12 06:00
MHDA- 2015/05/23 06:00
CRDT- 2014/07/12 06:00
PHST- 2014/07/12 06:00 [entrez]
PHST- 2014/07/12 06:00 [pubmed]
PHST- 2015/05/23 06:00 [medline]
AID - 10.1039/c4mb00240g [doi]
PST - ppublish
SO  - Mol Biosyst. 2014 Oct;10(10):2558-66. doi: 10.1039/c4mb00240g.