PMID- 25015608
OWN - NLM
STAT- MEDLINE
DCOM- 20150417
LR  - 20201005
IS  - 1735-546X (Electronic)
IS  - 1735-1308 (Linking)
VI  - 11
IP  - 3
DP  - 2014 Jul 8
TI  - Comparing supportive properties of poly lactic-co-glycolic acid (PLGA), 
      PLGA/collagen and human amniotic membrane for human urothelial and smooth muscle 
      cells engineering.
PG  - 1620-8
AB  - PURPOSE: To compare human urothelial and smooth muscle cells attachment and 
      proliferation using three different matrices; poly lactic-co-glycolic acid 
      (PLGA), PLGA/collagen and human amniotic membrane (hAM). MATERIALS AND METHODS: 
      Human urothelial and smooth muscle cells were cultured and examined for 
      expression of urothelium (pancytokeratin and uroplakin III) and smooth muscle 
      cells [desmin and alpha smooth muscle actin (alpha-SMA)] markers. Cells were cultured 
      on three scaffolds; PLGA, PLGA/collagen and hAM. Thereafter, they were analyzed 
      for cell growth on days 1, 3, 7, 14 and 21 after seeding by 3-(4, 
      5-dimethylthiazole-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Scaffolds 
      were fixed and processed for hematoxylin and eosin (H&E) staining and 
      immunohistochemistry against their cell specific markers after 7 and 14 days of 
      culture. RESULTS: MTT assay results revealed that collagen has improved cell 
      attachment features of PLGA and led to significant increase of MTT signal in 
      PLGA/collagen compared to PLGA (P < .001) and hAM (P < .001). hAM was a weaker 
      matrix for both cell types as demonstrated in MTT assay and scanning electron 
      microscope (SEM) images. SEM micrographs showed normal phenotype and distribution 
      on PLGA and PLGA/collagen. In the same line, cells formed a well-developed layer 
      either on PLGA or PLGA/collagen, which maintained expression of their 
      corresponding markers. CONCLUSION: Our findings demonstrated significant 
      improvement of cell attachment and growth achieved by collagen coating 
      (PLGA/collagen) compared to PLGA and hAM. hAM despite of its natural entity was a 
      weaker matrix for bladder engineering purposes.
FAU - Sharifiaghdas, Farzaneh
AU  - Sharifiaghdas F
AD  - Urology and Nephrology Research Center, Department of Urology, Shahid 
      Labbafinejad Medical Center, Shahid Beheshti University of Medical Sciences, 
      Tehran, Iran.
FAU - Naji, Mohammad
AU  - Naji M
AD  - Urology and Nephrology Research Center, Shahid Beheshti University of Medical 
      Sciences, Tehran, Iran.
FAU - Sarhangnejad, Reza
AU  - Sarhangnejad R
AD  - Urology and Nephrology Research Center, Department of Urology, Shahid 
      Labbafinejad Medical Center, Shahid Beheshti University of Medical Sciences, 
      Tehran, Iran.
FAU - Rajabi-Zeleti, Sareh
AU  - Rajabi-Zeleti S
AD  - Department of Stem Cells and Developmental Biology at Cell Science Research 
      Center, Royan Institute for Stem Cell Biology and Technology, Academic Center for 
      Education, Culture and Research, Tehran, Iran.
FAU - Mirzadeh, Hamid
AU  - Mirzadeh H
AD  - Faculty of Polymer and Petrochemistry, Iran Polymer and Petrochemical Institute, 
      Tehran, Iran.
FAU - Zandi, Mojgan
AU  - Zandi M
AD  - Biomaterials Department, Iran Polymer and Petrochemical Institute, Tehran, Iran.
FAU - Saeed, Mahdi
AU  - Saeed M
AD  - Biomaterials Department, Iran Polymer and Petrochemical Institute, Tehran, Iran.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140708
PL  - Iran
TA  - Urol J
JT  - Urology journal
JID - 101286676
RN  - 0 (Biocompatible Materials)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - *Amnion
MH  - Biocompatible Materials
MH  - Cell Adhesion
MH  - Cell Proliferation
MH  - *Collagen
MH  - Epithelial Cells/*physiology/ultrastructure
MH  - Humans
MH  - *Lactic Acid
MH  - Myocytes, Smooth Muscle/*physiology/ultrastructure
MH  - *Polyglycolic Acid
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Tissue Engineering/methods
MH  - *Tissue Scaffolds
MH  - Urinary Bladder
MH  - Urothelium
EDAT- 2014/07/13 06:00
MHDA- 2015/04/18 06:00
CRDT- 2014/07/13 06:00
PHST- 2013/06/10 00:00 [received]
PHST- 2014/02/16 00:00 [accepted]
PHST- 2014/07/13 06:00 [entrez]
PHST- 2014/07/13 06:00 [pubmed]
PHST- 2015/04/18 06:00 [medline]
AID - 2120 [pii]
PST - epublish
SO  - Urol J. 2014 Jul 8;11(3):1620-8.