PMID- 25016264
OWN - NLM
STAT- MEDLINE
DCOM- 20141020
LR  - 20140713
IS  - 1011-601X (Print)
IS  - 1011-601X (Linking)
VI  - 27
IP  - 4 Suppl
DP  - 2014 Jul
TI  - Effect of Pakistani medicinal plants on IgE/antigen- and ionophore-induced 
      mucosal mast cells degranulation.
PG  - 1041-8
AB  - Cumulative evidence has now demonstrated the stimulation of mucosal mast cells by 
      both allergic and non-allergic triggers and their inhibition as a potential 
      therapeutic target in many diseases like food allergy and ulcerative colitis. 
      Hence, we screened medicinal plants from Pakistan against antigen- and 
      ionophore-induced degranulation of mucosal mast cells. Aqueous ethanol extracts 
      were screened. IgE/antigen- and A23187-induced degranulation of mucosal-type 
      murine bone marrow derived mast cells (mBMMCs) were screening assays and 
      beta-hexosaminidase released from degranulated mBMMCs was measured. Real 
      time-polymerase chain reaction was employed to examine the expression of TNF-alpha 
      and IL-4 mRNA. Acetoxychavicol acetate, was examined by degranulation assays and 
      real time-PCR. Among the ten plants screened against IgE/antigen stimulated 
      degranulation, five plants; Alpinia galangal, Mentha arvensis, Myrtus communis, 
      Polygonum bistorta and Syzygium aromaticum demonstrated significant (p<0.01) 
      suppression of the degranulation at 100 mug/ml. Of them, Alpinia galangal showed 
      significant (p<0.01) inhibition at 32 mg/ml. In A23187-induced degranulation, all 
      plants showed significant (p<0.01) inhibition at 100 mug/ml except Tamarix dioica. 
      Again Alpinia galangal exhibited significant (p<0.01) suppression at 32 mug/ml. In 
      a concentration dependent assay, Alpinia galangal revealed significant 
      suppression at 10 mug/ml against A23187-stimulated degranulation. Acetoxychavicol 
      acetate demonstrated significant (p<0.01) inhibition at 3.2 muM in 
      IgE/antigen-treated cells and at 10 muM in A23187-treated cells. Furthermore, both 
      Alpinia galangal and acetoxychavicol acetate suppressed the IgE/antigen- and 
      A23187-enhanced mRNA expression of inflammatory cytokines, TNF-a and IL-4, in 
      mBMMCs. Our findings revealed the suppressive effect of Alpinia galangal and 
      acetoxychavicol acetate on degranulation of mBMMCs by allergic and non-allergic 
      stimuli, which can be utilized for future drug development against food allergy 
      or ulcerative colitis.
FAU - Zaidi, Syed Faisal
AU  - Zaidi SF
AD  - Division of Gastrointestinal Pathophysiology, Institute of Natural Medicine, 
      University of Toyama, Toyama, Japan / Department of Basic Medical Sciences, 
      College of Medicine, King Saud bin Abdulaziz University of Health Sciences, 
      Jeddah, Kingdom of Saudi Arabia.
FAU - Kim, Ji-Hyun
AU  - Kim JH
AD  - Division of Gastrointestinal Pathophysiology, Institute of Natural Medicine, 
      University of Toyama, Toyama, Japan.
FAU - Tomoe, Yashiro
AU  - Tomoe Y
AD  - Division of Gastrointestinal Pathophysiology, Institute of Natural Medicine, 
      University of Toyama, Toyama, Japan.
FAU - Usmanghani, Khan
AU  - Usmanghani K
AD  - Department of Clinical Pharmacy and Health Care, Jinnah University for Women, 
      Karachi, Pakistan.
FAU - Kadowaki, Makoto
AU  - Kadowaki M
AD  - Division of Gastrointestinal Pathophysiology, Institute of Natural Medicine, 
      University of Toyama, Toyama, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Pakistan
TA  - Pak J Pharm Sci
JT  - Pakistan journal of pharmaceutical sciences
JID - 9426356
RN  - 0 (Calcium Ionophores)
RN  - 0 (Cytokines)
RN  - 0 (Plant Extracts)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 37H9VM9WZL (Calcimycin)
SB  - IM
MH  - Animals
MH  - Calcimycin/*pharmacology
MH  - Calcium Ionophores/*pharmacology
MH  - Cell Degranulation/*drug effects
MH  - Cells, Cultured
MH  - Cytokines/genetics
MH  - Immunoglobulin E/*immunology
MH  - Mast Cells/*drug effects/physiology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Pakistan
MH  - Plant Extracts/*pharmacology
MH  - *Plants, Medicinal
EDAT- 2014/07/13 06:00
MHDA- 2014/10/21 06:00
CRDT- 2014/07/13 06:00
PHST- 2014/07/13 06:00 [entrez]
PHST- 2014/07/13 06:00 [pubmed]
PHST- 2014/10/21 06:00 [medline]
PST - ppublish
SO  - Pak J Pharm Sci. 2014 Jul;27(4 Suppl):1041-8.