PMID- 25017912
OWN - NLM
STAT- MEDLINE
DCOM- 20141104
LR  - 20211021
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 450
IP  - 4
DP  - 2014 Aug 8
TI  - Down-regulation of lipoprotein lipase increases ABCA1-mediated cholesterol efflux 
      in THP-1 macrophages.
PG  - 1416-21
LID - S0006-291X(14)01229-7 [pii]
LID - 10.1016/j.bbrc.2014.07.015 [doi]
AB  - The ATP-binding cassette transporter A1 (ABCA1) mediates the efflux of excess 
      cholesterol from foam cells to lipid-poor apolipoprotein A-I, in a process called 
      reverse cholesterol transport. Lipoprotein lipase (LPL) is a lipolytic enzyme 
      expressed by macrophages within atherosclerotic lesions. Lentivirus-mediated RNA 
      interference was used to genetically knock-down (KD) the expression of LPL in 
      THP-1 macrophages. Silencing of the LPL gene was confirmed by end-point PCR, real 
      time PCR, and protein analysis. Suppression of LPL expression correlated with a 
      1.6-fold up-regulation of ABCA1 mRNA levels, and resulted in a 4.5-fold increase 
      in ABCA1-dependent cholesterol efflux. Replenishing LPL by addition of purified 
      bovine LPL to the cell culture media resulted in down-regulation of 
      ABCA1-mediated cholesterol efflux in both wild-type and LPL knockdown cells. 
      These findings suggest an inverse correlation between macrophage LPL levels and 
      ABCA1 cholesterol transport activity.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Kawashima, Ryoko L
AU  - Kawashima RL
AD  - Department of Chemistry and Biochemistry, California State University Northridge, 
      Northridge, CA 91330-8262, United States.
FAU - Medh, Jheem D
AU  - Medh JD
AD  - Department of Chemistry and Biochemistry, California State University Northridge, 
      Northridge, CA 91330-8262, United States. Electronic address: 
      jheem.medh@csun.edu.
LA  - eng
GR  - R15 HL083946/HL/NHLBI NIH HHS/United States
GR  - SC3 GM095413/GM/NIGMS NIH HHS/United States
GR  - SC3GM095413/GM/NIGMS NIH HHS/United States
GR  - R15HL083946/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20140710
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (ABCA1 protein, human)
RN  - 0 (ATP Binding Cassette Transporter 1)
RN  - 0 (DNA Primers)
RN  - 97C5T2UQ7J (Cholesterol)
RN  - EC 3.1.1.34 (Lipoprotein Lipase)
SB  - IM
MH  - ATP Binding Cassette Transporter 1/*metabolism
MH  - Base Sequence
MH  - Biological Transport
MH  - Cell Line
MH  - Cholesterol/*metabolism
MH  - DNA Primers
MH  - Humans
MH  - Lipoprotein Lipase/*metabolism
MH  - Macrophages/*metabolism
MH  - Real-Time Polymerase Chain Reaction
PMC - PMC4142826
MID - NIHMS613003
OTO - NOTNLM
OT  - ABCA1 transporter
OT  - Cholesterol efflux
OT  - Lipoprotein lipase
OT  - Reverse cholesterol transport
OT  - THP-1 macrophages
OT  - shRNA
EDAT- 2014/07/16 06:00
MHDA- 2014/11/05 06:00
PMCR- 2015/08/08
CRDT- 2014/07/15 06:00
PHST- 2014/06/30 00:00 [received]
PHST- 2014/07/02 00:00 [accepted]
PHST- 2014/07/15 06:00 [entrez]
PHST- 2014/07/16 06:00 [pubmed]
PHST- 2014/11/05 06:00 [medline]
PHST- 2015/08/08 00:00 [pmc-release]
AID - S0006-291X(14)01229-7 [pii]
AID - 10.1016/j.bbrc.2014.07.015 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2014 Aug 8;450(4):1416-21. doi: 
      10.1016/j.bbrc.2014.07.015. Epub 2014 Jul 10.