PMID- 25019198
OWN - NLM
STAT- MEDLINE
DCOM- 20150916
LR  - 20211021
IS  - 1557-8534 (Electronic)
IS  - 1547-3287 (Print)
IS  - 1547-3287 (Linking)
VI  - 24
IP  - 1
DP  - 2015 Jan 1
TI  - Asymmetric aneuploidy in mesenchymal stromal cells detected by in situ 
      karyotyping and fluorescence in situ hybridization: suggestions for reference 
      values for stem cells.
PG  - 77-92
LID - 10.1089/scd.2014.0137 [doi]
AB  - Cytogenetic testing is important to ensure patient safety before therapeutic 
      application of mesenchymal stromal cells (MSCs). However, the standardized 
      methods and criteria for the screening of chromosomal abnormalities of MSCs have 
      not yet been determined. We investigated the frequency of cytogenetic aberrations 
      in MSCs using G-banding and fluorescence in situ hybridization (FISH) and suggest 
      reference values for aneuploidy in MSCs. Cytogenetic analysis was performed on 
      103 consecutive cultures from 68 MSCs (25 adipose-origin, 20 bone marrow-origin, 
      18 cord blood-origin, and 5 neural stem cells; 8 from adipose tissue of patients 
      with breast cancer and 60 from healthy donors). We compared the MSC aneuploidy 
      patterns with those of hematological malignancies and benign hematological 
      diseases. Interphase FISH showed variable aneuploid clone proportions (1%-20%) in 
      68 MSCs. The aneuploidy patterns were asymmetric, and aneuploidy of chromosomes 
      16, 17, 18, and X occurred most frequently. Clones with polysomy were 
      significantly more abundant than those with monosomy. The cutoff value of maximum 
      polysomy rates (upper 95th percentile value) was 13.0%. By G-banding, 5 of the 61 
      MSCs presented clonal chromosomal aberrations. Aneuploidy was asymmetric in the 
      malignant hematological diseases, while it was symmetric in the benign 
      hematological diseases. We suggest an aneuploidy cutoff value of 13%, and FISH 
      for aneuploidy of chromosomes 16, 17, 18, and X would be informative to evaluate 
      the genetic stability of MSCs. Although it is unclear whether the aneuploid 
      clones might represent the senescent cell population or transformed cells, more 
      attention should be focused on the safety of MSCs, and G-banding combined with 
      FISH should be performed.
FAU - Kim, Seon Young
AU  - Kim SY
AD  - 1 Department of Laboratory Medicine, Seoul National University College of 
      Medicine , Seoul, Republic of Korea.
FAU - Im, Kyongok
AU  - Im K
FAU - Park, Si Nae
AU  - Park SN
FAU - Kwon, Jiseok
AU  - Kwon J
FAU - Kim, Jung-Ah
AU  - Kim JA
FAU - Choi, Qute
AU  - Choi Q
FAU - Hwang, Sang Mee
AU  - Hwang SM
FAU - Han, Sung-Hee
AU  - Han SH
FAU - Kwon, Sunghoon
AU  - Kwon S
FAU - Oh, Il-Hoan
AU  - Oh IH
FAU - Lee, Dong Soon
AU  - Lee DS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Stem Cells Dev
JT  - Stem cells and development
JID - 101197107
SB  - IM
MH  - *Aneuploidy
MH  - *Chromosome Banding
MH  - Hematologic Neoplasms/metabolism/*pathology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *Karyotyping
MH  - Mesenchymal Stem Cells/metabolism/*pathology
PMC - PMC4273192
EDAT- 2014/07/16 06:00
MHDA- 2015/09/17 06:00
PMCR- 2016/01/01
CRDT- 2014/07/15 06:00
PHST- 2014/07/15 06:00 [entrez]
PHST- 2014/07/16 06:00 [pubmed]
PHST- 2015/09/17 06:00 [medline]
PHST- 2016/01/01 00:00 [pmc-release]
AID - 10.1089/scd.2014.0137 [pii]
AID - 10.1089/scd.2014.0137 [doi]
PST - ppublish
SO  - Stem Cells Dev. 2015 Jan 1;24(1):77-92. doi: 10.1089/scd.2014.0137.