PMID- 2502175
OWN - NLM
STAT- MEDLINE
DCOM- 19890829
LR  - 20190613
IS  - 0006-2960 (Print)
IS  - 0006-2960 (Linking)
VI  - 28
IP  - 9
DP  - 1989 May 2
TI  - Serine-324 of myosin's heavy chain is photoaffinity-labeled by 
      3'(2')-O-(4-benzoylbenzoyl)adenosine triphosphate.
PG  - 3989-95
AB  - A portion of the active site of rabbit skeletal myosin near the ribose ring of 
      ATP can be labeled by the photoaffinity analogue 
      3'(2')-O-(4-benzoylbenzoyl)adenosine triphosphate (Bz2ATP). The specificity of 
      the photolabeling was assured by first trapping [14C]Bz2ATP at the active site by 
      use of thiol cross-linking agents [Mahmood, R., Cremo, C., Nakamaye, K., & Yount, 
      R. (1987) J. Biol. Chem. 262, 14479-14486]. Five radioactive peptides were 
      isolated by high-performance liquid chromatography after extensive trypsin and 
      subtilisin digestion of photolabeled myosin subfragment 1. Four of these peptides 
      were sequenced by Edman techniques, and all originated from a region with the 
      sequence Gly-Glu-Ile-Thr-Val-Pro-Ser-Ile-Asp-Asp-Gln, which corresponds to rabbit 
      myosin heavy chain residues 318-328. The fifth labeled peptide had an amino acid 
      composition appropriate for residues 312-328. Amino acid composition, 
      radiochemical analysis, and sequence data indicate that Ser-324 is the major 
      amino acid residue photolabeled by Bz2ATP. Spectrophotometric evidence indicates 
      that the benzophenone carbonyl group has inserted into a C-H bond from either the 
      alpha- or beta-carbon of serine. These results place Ser-324 at a distance of 6-7 
      A from the 3'(2') ribose oxygens of ATP bound at the active site of myosin.
FAU - Mahmood, R
AU  - Mahmood R
AD  - Institute of Biological Chemistry, Washington State University, Pullman 
      99164-4660.
FAU - Elzinga, M
AU  - Elzinga M
FAU - Yount, R G
AU  - Yount RG
LA  - eng
GR  - DK05195/DK/NIDDK NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biochemistry
JT  - Biochemistry
JID - 0370623
RN  - 0 (Affinity Labels)
RN  - 0 (Amino Acids)
RN  - 0 (Myosin Subfragments)
RN  - 0 (Peptide Fragments)
RN  - 452VLY9402 (Serine)
RN  - 4P5DXU1F8Q (3'-O-(4-benzoyl)benzoyladenosine 5'-triphosphate)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 3.6.4.1 (Myosins)
SB  - IM
MH  - Adenosine Triphosphate/*analogs & derivatives/metabolism
MH  - Affinity Labels/*metabolism
MH  - Amino Acid Sequence
MH  - Amino Acids/analysis
MH  - Animals
MH  - Chromatography, Gel
MH  - Chromatography, High Pressure Liquid
MH  - Molecular Sequence Data
MH  - Muscles/metabolism
MH  - Myosin Subfragments
MH  - Myosins/*metabolism
MH  - Peptide Fragments/isolation & purification/*metabolism
MH  - Rabbits
MH  - *Serine
MH  - Spectrophotometry, Ultraviolet
EDAT- 1989/05/02 00:00
MHDA- 1989/05/02 00:01
CRDT- 1989/05/02 00:00
PHST- 1989/05/02 00:00 [pubmed]
PHST- 1989/05/02 00:01 [medline]
PHST- 1989/05/02 00:00 [entrez]
AID - 10.1021/bi00435a054 [doi]
PST - ppublish
SO  - Biochemistry. 1989 May 2;28(9):3989-95. doi: 10.1021/bi00435a054.