PMID- 25025314 OWN - NLM STAT- MEDLINE DCOM- 20150709 LR - 20221207 IS - 1873-3557 (Electronic) IS - 1386-1425 (Linking) VI - 134 DP - 2015 Jan 5 TI - Enhanced fluorescence quenching in an acridine orange - alizarin red system through matrine and its analytical application. PG - 413-8 LID - S1386-1425(14)01014-2 [pii] LID - 10.1016/j.saa.2014.06.115 [doi] AB - This study shows that alizarin red (AR) only slightly quenched fluorescence for acridine orange (AO) in an AR/AO mixed solution at pH=5-6. The reduced fluorescent signal was closely and linearly associated with the level of MT added to the system, which is the basis for a new quantitative MT assay method using the fluorescence quenching reaction in the AO-AR system. The results show that under optimal conditions, this method had a 14.9-43.5 mg L(-1) linear detection range with a 1.38 mg L(-1) detection limit and 1.24% precision. In addition, this method was used to determine the MT levels in the commercially available MT-containing pesticides and suppositories, which showed a 96.6-103% recovery. Therefore, this method has multiple advantages, including simple and fast operation, high accuracy and low cost. Moreover, herein, we investigated the underlying mechanism in-depth using an ultraviolet (UV) spectroscopic technique. CI - Copyright (c) 2014 Elsevier B.V. All rights reserved. FAU - Wei, Xiaoling AU - Wei X AD - College of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China. FAU - Wang, Xiaojun AU - Wang X AD - College of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China. FAU - Gong, Qi AU - Gong Q AD - College of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China. FAU - Wang, Lisheng AU - Wang L AD - College of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China. Electronic address: wxl2558@163.com. FAU - Zhou, Shiwu AU - Zhou S AD - College of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China. LA - eng PT - Evaluation Study PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20140627 PL - England TA - Spectrochim Acta A Mol Biomol Spectrosc JT - Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy JID - 9602533 RN - 0 (Alkaloids) RN - 0 (Anthraquinones) RN - 0 (Fluorescent Dyes) RN - 0 (Quinolizines) RN - 60MEW57T9G (alizarin) RN - F30N4O6XVV (Acridine Orange) RN - 0 (Matrines) SB - IM MH - Acridine Orange/*chemistry MH - Alkaloids/*analysis MH - Anthraquinones/*chemistry MH - Fluorescence MH - Fluorescent Dyes/*chemistry MH - Limit of Detection MH - Quinolizines/*analysis MH - Spectrometry, Fluorescence/methods MH - Matrines OTO - NOTNLM OT - Acridine orange (AO) OT - Alizarin red (AR) OT - Energy transfer OT - Fluorescence quenching OT - Matrine EDAT- 2014/07/16 06:00 MHDA- 2015/07/15 06:00 CRDT- 2014/07/16 06:00 PHST- 2013/11/21 00:00 [received] PHST- 2014/06/16 00:00 [revised] PHST- 2014/06/18 00:00 [accepted] PHST- 2014/07/16 06:00 [entrez] PHST- 2014/07/16 06:00 [pubmed] PHST- 2015/07/15 06:00 [medline] AID - S1386-1425(14)01014-2 [pii] AID - 10.1016/j.saa.2014.06.115 [doi] PST - ppublish SO - Spectrochim Acta A Mol Biomol Spectrosc. 2015 Jan 5;134:413-8. doi: 10.1016/j.saa.2014.06.115. Epub 2014 Jun 27.