PMID- 25025397 OWN - NLM STAT- MEDLINE DCOM- 20151208 LR - 20211021 IS - 1528-3658 (Electronic) IS - 1076-1551 (Print) IS - 1076-1551 (Linking) VI - 20 IP - 1 DP - 2014 Aug 14 TI - Maternal magnesium deficiency in mice leads to maternal metabolic dysfunction and altered lipid metabolism with fetal growth restriction. PG - 332-40 LID - 10.2119/molmed.2014.00137 [doi] AB - Inadequate magnesium (Mg) intake is a widespread problem, with over 50% of women of reproductive age consuming less than the Recommended Dietary Allowance (RDA). Because pregnancy increases the requirement for Mg and the beneficial effects of magnesium sulfate for preeclampsia/eclampsia and fetal neuroprotection are well described, we examined the outcomes of Mg deficiency during pregnancy. Briefly, pregnant Swiss Webster mice were fed either control or Mg-deficient diets starting on gestational day (GD) 6 through euthanasia on GD17. Mg-deficient dams had significantly reduced weight gain and higher plasma adipokines, in the absence of inflammation. Livers of Mg-deficient dams had significantly higher saturated fatty acids (SFAs) and monounsaturated fatty acids (MUFAs) and lower polyunsaturated fatty acids (PUFAs), including docosahexaenoic acid (DHA) (P < 0.0001) and arachidonic acid (AA) (P < 0.0001). Mechanistically, Mg deficiency was accompanied by enhanced desaturase and elongase mRNA expression in maternal livers along with higher circulating insulin and glucose concentrations (P < 0.05) and increased mRNA expression of Srebf1 and Chrebp, regulators of fatty acid synthesis (P < 0.05). Fetal pups exposed to Mg deficiency were growth-restricted and exhibited reduced survival. Mg-deficient fetal livers showed lower MUFAs and higher PUFAs, with lower desaturase and elongase mRNA expression than controls. In addition, DHA concentrations were lower in Mg-deficient fetal brains (P < 0.05). These results indicate that Mg deficiency during pregnancy influences both maternal and fetal fatty acid metabolism, fetal growth and fetal survival, and support better understanding maternal Mg status before and during pregnancy. FAU - Gupta, Madhu AU - Gupta M AD - Elmezzi Graduate School of Molecular Medicine, Manhasset, New York, United States of America The Feinstein Institute for Medical Research, The Center for Immunology and Inflammation, Manhasset, New York, United States of America. FAU - Solanki, Malvika H AU - Solanki MH AD - Elmezzi Graduate School of Molecular Medicine, Manhasset, New York, United States of America The Feinstein Institute for Medical Research, The Center for Immunology and Inflammation, Manhasset, New York, United States of America. FAU - Chatterjee, Prodyot K AU - Chatterjee PK AD - The Feinstein Institute for Medical Research, The Center for Immunology and Inflammation, Manhasset, New York, United States of America. FAU - Xue, Xiangying AU - Xue X AD - The Feinstein Institute for Medical Research, The Center for Immunology and Inflammation, Manhasset, New York, United States of America. FAU - Roman, Amanda AU - Roman A AD - Hofstra North Shore-LIJ School of Medicine, Division of Maternal-Fetal Medicine, Manhasset, New York, United States of America. FAU - Desai, Neeraj AU - Desai N AD - Hofstra North Shore-LIJ School of Medicine, Division of Maternal-Fetal Medicine, Manhasset, New York, United States of America. FAU - Rochelson, Burton AU - Rochelson B AD - Hofstra North Shore-LIJ School of Medicine, Division of Maternal-Fetal Medicine, Manhasset, New York, United States of America. FAU - Metz, Christine N AU - Metz CN AD - Elmezzi Graduate School of Molecular Medicine, Manhasset, New York, United States of America The Feinstein Institute for Medical Research, The Center for Immunology and Inflammation, Manhasset, New York, United States of America Hofstra North Shore-LIJ School of Medicine, Division of Maternal-Fetal Medicine, Manhasset, New York, United States of America. LA - eng GR - U24 DK059637/DK/NIDDK NIH HHS/United States GR - DK59637/DK/NIDDK NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't DEP - 20140814 PL - England TA - Mol Med JT - Molecular medicine (Cambridge, Mass.) JID - 9501023 RN - 0 (Adiponectin) RN - 0 (Basic Helix-Loop-Helix Leucine Zipper Transcription Factors) RN - 0 (Blood Glucose) RN - 0 (Cytokines) RN - 0 (Fatty Acids) RN - 0 (Insulin) RN - 0 (Leptin) RN - 0 (Mlxipl protein, mouse) RN - 0 (Nuclear Proteins) RN - 0 (RNA, Messenger) RN - 0 (Srebf1 protein, mouse) RN - 0 (Sterol Regulatory Element Binding Protein 1) RN - 0 (Transcription Factors) RN - I38ZP9992A (Magnesium) SB - IM MH - Adiponectin/blood/metabolism MH - Amniotic Fluid/metabolism MH - Animals MH - Basic Helix-Loop-Helix Leucine Zipper Transcription Factors MH - Blood Glucose/analysis MH - Cytokines/blood/metabolism MH - Fatty Acids/metabolism MH - Female MH - Fetal Development MH - Fetal Growth Retardation/blood/metabolism MH - Insulin/metabolism MH - Leptin/blood/metabolism MH - Liver/metabolism MH - Magnesium/blood/metabolism MH - Magnesium Deficiency/*metabolism MH - Metabolic Diseases/blood/*metabolism MH - Mice MH - Nuclear Proteins/genetics MH - Pregnancy/blood/*metabolism MH - RNA, Messenger/metabolism MH - Sterol Regulatory Element Binding Protein 1/genetics MH - Transcription Factors/genetics PMC - PMC4153836 EDAT- 2014/07/16 06:00 MHDA- 2015/12/15 06:00 PMCR- 2014/08/14 CRDT- 2014/07/16 06:00 PHST- 2014/07/10 00:00 [received] PHST- 2014/07/11 00:00 [accepted] PHST- 2014/07/16 06:00 [entrez] PHST- 2014/07/16 06:00 [pubmed] PHST- 2015/12/15 06:00 [medline] PHST- 2014/08/14 00:00 [pmc-release] AID - molmed.2014.00137 [pii] AID - 14_137_gupta [pii] AID - 10.2119/molmed.2014.00137 [doi] PST - epublish SO - Mol Med. 2014 Aug 14;20(1):332-40. doi: 10.2119/molmed.2014.00137.