PMID- 25025927
OWN - NLM
STAT- MEDLINE
DCOM- 20141009
LR  - 20140818
IS  - 1872-7980 (Electronic)
IS  - 0304-3835 (Linking)
VI  - 352
IP  - 2
DP  - 2014 Oct 1
TI  - The ERK/eIF4F/Bcl-XL pathway mediates SGP-2 induced osteosarcoma cells apoptosis 
      in vitro and in vivo.
PG  - 203-13
LID - S0304-3835(14)00331-0 [pii]
LID - 10.1016/j.canlet.2014.06.015 [doi]
AB  - The aim of this study is to assess the molecular foundation of anti-tumor 
      activity of SGP-2 in osteosarcoma cells. SGP-2 significantly blocks cell 
      proliferation in human osteosarcoma U2OS cell model and inhibits tumor growth 
      without causing apparent toxicity effect in mouse sarcoma S-180 cell-derived 
      tumor model. Moreover, SGP-2 induces intrinsic apoptosis including the activation 
      of caspase-3/7/9, the loss of mitochondrial transmembrane potential (DeltaPsim), and 
      the release of cytochrome c from mitochondrion, controlled by the down-regulation 
      of B-cell lymphoma-extra large (Bcl-XL). Further research reveals that SGP-2 
      inhibits the assembly of eukaryotic initiation factor 4F (eIF4F) complex which is 
      responsible for the decline of Bcl-XL. Finally, extracellular-signal-regulated 
      kinase (ERK) controls SGP-2 induced intrinsic apoptosis. Taken together, SGP-2 
      exerts anti-tumor effect through intrinsic apoptotic pathway controlled by 
      ERK/eIF4F/Bcl-XL pathway.
CI  - Copyright (c) 2014 Elsevier Ireland Ltd. All rights reserved.
FAU - Zhang, Zhenzhen
AU  - Zhang Z
AD  - State Key Laboratory of Natural Medicines, China Pharmaceutical University, 
      Jiangsu, China; School of Life Science and Technology, China Pharmaceutical 
      University, Jiangsu, China.
FAU - Zheng, Ying
AU  - Zheng Y
AD  - State Key Laboratory of Natural Medicines, China Pharmaceutical University, 
      Jiangsu, China; School of Life Science and Technology, China Pharmaceutical 
      University, Jiangsu, China.
FAU - Zhu, Rui
AU  - Zhu R
AD  - State Key Laboratory of Natural Medicines, China Pharmaceutical University, 
      Jiangsu, China; School of Life Science and Technology, China Pharmaceutical 
      University, Jiangsu, China.
FAU - Zhu, Yiqing
AU  - Zhu Y
AD  - State Key Laboratory of Natural Medicines, China Pharmaceutical University, 
      Jiangsu, China; School of Life Science and Technology, China Pharmaceutical 
      University, Jiangsu, China.
FAU - Yao, Wenbing
AU  - Yao W
AD  - State Key Laboratory of Natural Medicines, China Pharmaceutical University, 
      Jiangsu, China; School of Life Science and Technology, China Pharmaceutical 
      University, Jiangsu, China.
FAU - Liu, Wei
AU  - Liu W
AD  - State Key Laboratory of Natural Medicines, China Pharmaceutical University, 
      Jiangsu, China; School of Life Science and Technology, China Pharmaceutical 
      University, Jiangsu, China; Department of Food Quality and Safety, China 
      Pharmaceutical University, Jiangsu, China.
FAU - Gao, Xiangdong
AU  - Gao X
AD  - State Key Laboratory of Natural Medicines, China Pharmaceutical University, 
      Jiangsu, China; School of Life Science and Technology, China Pharmaceutical 
      University, Jiangsu, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140712
PL  - Ireland
TA  - Cancer Lett
JT  - Cancer letters
JID - 7600053
RN  - 0 (Antineoplastic Agents, Phytogenic)
RN  - 0 (BCL2L1 protein, human)
RN  - 0 (Eukaryotic Initiation Factor-4F)
RN  - 0 (Polysaccharides)
RN  - 0 (bcl-X Protein)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
RN  - EC 3.4.22.- (Caspases)
SB  - IM
MH  - Animals
MH  - Antineoplastic Agents, Phytogenic/*pharmacology/toxicity
MH  - Apoptosis/*drug effects
MH  - Bone Neoplasms/*drug therapy/genetics/metabolism/pathology
MH  - Caspases/metabolism
MH  - Cell Line, Tumor
MH  - Cell Proliferation/drug effects
MH  - Dose-Response Relationship, Drug
MH  - Enzyme Activation
MH  - Eukaryotic Initiation Factor-4F/genetics/*metabolism
MH  - Extracellular Signal-Regulated MAP Kinases/*metabolism
MH  - Humans
MH  - Male
MH  - Membrane Potential, Mitochondrial/drug effects
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Osteosarcoma/*drug therapy/genetics/metabolism/pathology
MH  - Polysaccharides/*pharmacology/toxicity
MH  - RNA Interference
MH  - Signal Transduction/drug effects
MH  - Time Factors
MH  - Transfection
MH  - Tumor Burden/drug effects
MH  - bcl-X Protein/genetics/*metabolism
OTO - NOTNLM
OT  - Apoptosis
OT  - ERK
OT  - Osteosarcoma
OT  - eIF4F
EDAT- 2014/07/16 06:00
MHDA- 2014/10/10 06:00
CRDT- 2014/07/16 06:00
PHST- 2014/04/16 00:00 [received]
PHST- 2014/06/16 00:00 [revised]
PHST- 2014/06/24 00:00 [accepted]
PHST- 2014/07/16 06:00 [entrez]
PHST- 2014/07/16 06:00 [pubmed]
PHST- 2014/10/10 06:00 [medline]
AID - S0304-3835(14)00331-0 [pii]
AID - 10.1016/j.canlet.2014.06.015 [doi]
PST - ppublish
SO  - Cancer Lett. 2014 Oct 1;352(2):203-13. doi: 10.1016/j.canlet.2014.06.015. Epub 
      2014 Jul 12.