PMID- 25026501
OWN - NLM
STAT- MEDLINE
DCOM- 20141003
LR  - 20140802
IS  - 1879-0038 (Electronic)
IS  - 0378-1119 (Linking)
VI  - 548
IP  - 2
DP  - 2014 Sep 15
TI  - Duplication and differentiation of common carp (Cyprinus carpio) myoglobin genes 
      revealed by BAC analysis.
PG  - 210-6
LID - S0378-1119(14)00817-8 [pii]
LID - 10.1016/j.gene.2014.07.034 [doi]
AB  - Two distinct myoglobin (mb) transcripts have been reported in common carp, 
      Cyprinus carpio, which is a hypoxia-tolerant fish living in habitats with greatly 
      fluctuant dissolved oxygen levels. Recombinant protein analysis has shown 
      functional specialization of the two mb transcripts. In this work, analysis for 
      mb-containing bacterial artificial chromosome (BAC) clones indicated different 
      genome loci for common carp myoglobin-1 (mb-1) and myoglobin-2 (mb-2) genes. 
      Fluorescence in situ hybridization (FISH) revealed that mb-1 and mb-2 are located 
      on separate chromosomes. In both of the mb-1 and mb-2 containing BAC clones, gene 
      synteny was well conserved with the homologous region on zebrafish chromosome 1, 
      supporting that the common carp specific mb-2 gene originated from the recent 
      whole genome duplication event in cyprinid lineage. Transcription factor binding 
      sites search indicated that both common carp mb genes lacked specificity Protein 
      1 (Sp1) and myocyte enhancer factor-2 (MEF2) binding sites, which mediated 
      muscle-specific and calcium-dependent expression in the well-studied mb 
      promoters. Potential hypoxia response elements (HREs) were predicted in the 
      regulatory region of common carp mb genes. These characteristics of common carp 
      mb gene regulatory region well interpreted the hypoxia-inducible, non-muscle 
      expression pattern of mb-1. In the case of mb-2, a 10 bp insertion to the binding 
      site of upstream stimulatory factor (USF), which was a co-factor of hypoxia 
      inducible factor (HIF), might cause the non-response to hypoxia treatment of 
      mb-2. The case of common carp mb gene duplication and subsequent differentiation 
      in expression pattern and protein function provided an example for adaptive 
      evolution toward aquatic hypoxia tolerance.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Zhao, Zi-Xia
AU  - Zhao ZX
AD  - The Center for Applied Aquatic Genomics, Chinese Academy of Fishery Sciences, 
      Beijing 100141, PR China.
FAU - Xu, Peng
AU  - Xu P
AD  - The Center for Applied Aquatic Genomics, Chinese Academy of Fishery Sciences, 
      Beijing 100141, PR China.
FAU - Cao, Ding-Chen
AU  - Cao DC
AD  - Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery 
      Sciences, Harbin 150070, PR China.
FAU - Kuang, You-Yi
AU  - Kuang YY
AD  - Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery 
      Sciences, Harbin 150070, PR China.
FAU - Deng, Hai-Xia
AU  - Deng HX
AD  - The Center for Applied Aquatic Genomics, Chinese Academy of Fishery Sciences, 
      Beijing 100141, PR China.
FAU - Zhang, Yan
AU  - Zhang Y
AD  - The Center for Applied Aquatic Genomics, Chinese Academy of Fishery Sciences, 
      Beijing 100141, PR China.
FAU - Xu, Li-Ming
AU  - Xu LM
AD  - The Center for Applied Aquatic Genomics, Chinese Academy of Fishery Sciences, 
      Beijing 100141, PR China.
FAU - Li, Jiong-Tang
AU  - Li JT
AD  - The Center for Applied Aquatic Genomics, Chinese Academy of Fishery Sciences, 
      Beijing 100141, PR China.
FAU - Xu, Jian
AU  - Xu J
AD  - The Center for Applied Aquatic Genomics, Chinese Academy of Fishery Sciences, 
      Beijing 100141, PR China.
FAU - Sun, Xiao-Wen
AU  - Sun XW
AD  - The Center for Applied Aquatic Genomics, Chinese Academy of Fishery Sciences, 
      Beijing 100141, PR China; Heilongjiang River Fisheries Research Institute, 
      Chinese Academy of Fishery Sciences, Harbin 150070, PR China. Electronic address: 
      sunxw2002@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140712
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (Fish Proteins)
RN  - 0 (Myoglobin)
SB  - IM
MH  - Animals
MH  - Binding Sites/physiology
MH  - Carps/*genetics
MH  - Cell Hypoxia/genetics/physiology
MH  - Chromosomes
MH  - Chromosomes, Artificial, Bacterial/genetics
MH  - Cyprinidae/genetics
MH  - Evolution, Molecular
MH  - Fish Proteins/*genetics/metabolism
MH  - *Gene Duplication
MH  - Genetic Speciation
MH  - Genome
MH  - Myoglobin/*genetics/metabolism
MH  - Promoter Regions, Genetic
MH  - Synteny
MH  - Zebrafish/genetics
OTO - NOTNLM
OT  - Common carp
OT  - Differentiation
OT  - Duplication
OT  - Hypoxia
OT  - Myoglobin
EDAT- 2014/07/16 06:00
MHDA- 2014/10/04 06:00
CRDT- 2014/07/16 06:00
PHST- 2013/07/24 00:00 [received]
PHST- 2014/05/09 00:00 [revised]
PHST- 2014/07/11 00:00 [accepted]
PHST- 2014/07/16 06:00 [entrez]
PHST- 2014/07/16 06:00 [pubmed]
PHST- 2014/10/04 06:00 [medline]
AID - S0378-1119(14)00817-8 [pii]
AID - 10.1016/j.gene.2014.07.034 [doi]
PST - ppublish
SO  - Gene. 2014 Sep 15;548(2):210-6. doi: 10.1016/j.gene.2014.07.034. Epub 2014 Jul 
      12.