PMID- 25026550
OWN - NLM
STAT- MEDLINE
DCOM- 20141104
LR  - 20161125
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 450
IP  - 4
DP  - 2014 Aug 8
TI  - Down-regulation of Homer1b/c attenuates group I metabotropic glutamate receptors 
      dependent Ca(2)(+) signaling through regulating endoplasmic reticulum Ca(2)(+) release in 
      PC12 cells.
PG  - 1568-74
LID - S0006-291X(14)01271-6 [pii]
LID - 10.1016/j.bbrc.2014.07.044 [doi]
AB  - The molecular basis for group I metabotropic glutamate receptors (mGluR1 and 5) 
      coupling to membrane ion channels and intracellular calcium pools is not fully 
      understood. Homer is a family of post synaptic density proteins functionally and 
      physically attached to target proteins at proline-rich sequences. In the present 
      study, we demonstrate that Homer1b/c is constitutively expressed in PC12 cells, 
      whereas Homer1a, the immediate early gene product, can be up-regulated by brain 
      derived neurotrophic factor (BDNF) and glutamate. Knockdown of Homer1b/c using 
      specific target small interfering RNA (siRNA) did not interfere the expression of 
      mGluR1, mGluR5 and their downstream effectors, including 
      inositol-1,4,5-trisphosphate receptors (IP3R), phospholipase C (PLC) and Gq 
      proteins. By analyzing Ca(2+) imaging in PC12 cells, we demonstrated that 
      Homer1b/c is an essential regulator of the Ca(2+) release from the endoplasmic 
      reticulum (ER) induced by the activation of group I mGluRs, IP3R and ryanodine 
      receptors (RyR). Furthermore, the group I mGluRs activation-dependent refilling 
      of the Ca(2+) stores in both resting and depolarizing conditions were strongly 
      attenuated in the absence of Homer1b/c. Together, our results demonstrate that in 
      PC12 cells Homer1b/c is a regulator of group I mGluRs related Ca(2+) homeostasis 
      that is essential for the maintenance of normal Ca(2+) levels in the ER.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Lv, Miao-Miao
AU  - Lv MM
AD  - Department of Anesthesiology, Tangdu Hospital of the Fourth Military Medical 
      University, Xi'an, Shaanxi 710032, China; Department of Anesthesiology, The 323 
      Hospital of PLA, Xi'an, Shaanxi 710054, China.
FAU - Cheng, Yong-Chun
AU  - Cheng YC
AD  - Department of Anesthesiology, The Third Hospital of PLA, Baoji, Shaanxi 721004, 
      China.
FAU - Xiao, Zhi-Bin
AU  - Xiao ZB
AD  - Department of Anesthesiology, The 323 Hospital of PLA, Xi'an, Shaanxi 710054, 
      China.
FAU - Sun, Mei-Yan
AU  - Sun MY
AD  - Department of Anesthesiology, Tangdu Hospital of the Fourth Military Medical 
      University, Xi'an, Shaanxi 710032, China.
FAU - Ren, Peng-Cheng
AU  - Ren PC
AD  - Department of Anesthesiology, Tangdu Hospital of the Fourth Military Medical 
      University, Xi'an, Shaanxi 710032, China.
FAU - Sun, Xu-De
AU  - Sun XD
AD  - Department of Anesthesiology, Tangdu Hospital of the Fourth Military Medical 
      University, Xi'an, Shaanxi 710032, China. Electronic address: 
      fmmusunxude@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140712
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA Primers)
RN  - 0 (Homer Scaffolding Proteins)
RN  - 0 (Receptors, Metabotropic Glutamate)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - *Calcium Signaling
MH  - Carrier Proteins/*metabolism
MH  - DNA Primers
MH  - *Down-Regulation
MH  - Endoplasmic Reticulum/*metabolism
MH  - Homer Scaffolding Proteins
MH  - PC12 Cells
MH  - RNA Interference
MH  - Rats
MH  - Real-Time Polymerase Chain Reaction
MH  - Receptors, Metabotropic Glutamate/*metabolism
OTO - NOTNLM
OT  - Calcium
OT  - Endoplasmic reticulum
OT  - Homer
OT  - RNAi
OT  - mGluRs
EDAT- 2014/07/16 06:00
MHDA- 2014/11/05 06:00
CRDT- 2014/07/16 06:00
PHST- 2014/06/29 00:00 [received]
PHST- 2014/07/07 00:00 [accepted]
PHST- 2014/07/16 06:00 [entrez]
PHST- 2014/07/16 06:00 [pubmed]
PHST- 2014/11/05 06:00 [medline]
AID - S0006-291X(14)01271-6 [pii]
AID - 10.1016/j.bbrc.2014.07.044 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2014 Aug 8;450(4):1568-74. doi: 
      10.1016/j.bbrc.2014.07.044. Epub 2014 Jul 12.