PMID- 25027430
OWN - NLM
STAT- MEDLINE
DCOM- 20150408
LR  - 20140716
IS  - 2095-4352 (Print)
VI  - 26
IP  - 7
DP  - 2014 Jul
TI  - [Analysis of components of mesenteric lymph in rats with severe intraperitoneal 
      infection].
PG  - 503-7
LID - 10.3760/cma.j.issn.2095-4352.2014.07.012 [doi]
AB  - OBJECTIVE: To investigate the components of mesenteric lymph of the rats with 
      severe intraperitoneal infection, and inquire into the effect of intestinal 
      lymphatic pathway in severe intraperitoneal infection. METHODS: Twenty-four male 
      Wistar rats were randomly divided into two groups according to random number 
      table method, namely model group and sham group with 12 rats in each group. The 
      rat model of severe intraperitoneal infection was reproduced by injecting 
      artificial gastric juice and E.coli intraperitoneally. Mesenteric lymph in both 
      groups was collected 4 hours after the reproduction of the model, and white blood 
      cells were counted and classified. The levels of endotoxin, alkaline phosphatase 
      (AKP), lactate dehydrogenase (LDH), creatine kinase (CK), glutamine transferase 
      (GST), protein and cytokines of mesenteric lymph were determined. RESULTS: 
      Compared with the sham group, there was an increase in the neutrophil ratio in 
      mesenteric lymph (0.167+/-0.004 vs. 0.610+/-0.006, t=33.520, P<0.001), however the 
      percentage of both macrophages (0.009+/-0.001 vs. 0.020+/-0.004, t=-6.677, P<0.001) 
      and lymphocytes (0.824+/-0.005 vs. 0.921+/-0.004, t=-31.471, P<0.001) was decreased 
      in model group. Compared with sham group, the levels of endotoxin (0.346+/-0.022 
      kEU/L vs. 0.186+/-0.001 kEU/L, t=18.103, P<0.001), AKP [U (king unit): 13.97+/-5.55 
      vs. 3.76+/-0.18, t=4.503, P=0.006], LDH (2 827.45+/-1 940.32 U/L vs. 712.68+/-14.09 
      U/L, t=2.670, P=0.044), CK (2.19+/-1.21 kU/L vs. 0.70+/-0.01 kU/L, t=3.035, P=0.029), 
      GST (12.33+/-6.53 kU/L vs. 1.36+/-0.39 kU/L, t=4.105, P=0.009) were all significantly 
      elevated. The concentration of protein (4.40+/-0.48 g/L vs. 2.84+/-0.16 g/L, t=6.882, 
      P=0.001), tumor necrosis factor-alpha (TNF-alpha: 499.39+/-76.36 ng/L vs. 180.90+/-70.98 
      ng/L, t=7.483, P<0.001), interleukin-6 (IL-6: 13.74+/-0.78 mug/L vs. -0.07+/-0.07 
      mug/L, t=52.972, P<0.001), intercellular adhesion molecule-1 (ICAM-1: 2 
      754.19+/-221.48 ng/L vs. 1 362.85+/-393.43 ng/L, t=6.891, P<0.001) and monocyte 
      chemo-attractant protein-1 (MCP-1: 28.23+/-1.77 mug/L vs. 24.87+/-1.15 mug/L, t=3.561, 
      P=0.007) and high mobility group protein-1 (HMGB-1: 1 392.78+/-572.42 ng/L vs. 
      564.17+/-21.32 ng/L, t=3.543, P=0.016) in mesenteric lymph in model group were 
      significantly higher than those in sham group. CONCLUSIONS: Intestinal lymphatic 
      pathway maybe the early pathway for the production of remote organ injury caused 
      by severe intraperitoneal infection.
FAU - Zhang, Yanmin
AU  - Zhang Y
AD  - Departemnt of Critical Care Medicine, Tianjin Nankai Hospital, Tianjin 300100, 
      China. Corresponding author: Cui Naiqiang, Email: nctsui@126.com.
FAU - Cui, Naiqiang
AU  - Cui N
FAU - Zhang, Shukun
AU  - Zhang S
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
JT  - Zhonghua wei zhong bing ji jiu yi xue
JID - 101604552
RN  - 0 (HMGB1 Protein)
RN  - 0 (Hbp1 protein, rat)
RN  - 0 (Interleukin-6)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
SB  - IM
MH  - Animals
MH  - Disease Models, Animal
MH  - HMGB1 Protein/metabolism
MH  - Intercellular Adhesion Molecule-1/metabolism
MH  - Interleukin-6/metabolism
MH  - Intraabdominal Infections/*metabolism
MH  - Lymph/chemistry
MH  - Male
MH  - Rats
MH  - Rats, Wistar
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2014/07/17 06:00
MHDA- 2015/04/09 06:00
CRDT- 2014/07/17 06:00
PHST- 2014/07/17 06:00 [entrez]
PHST- 2014/07/17 06:00 [pubmed]
PHST- 2015/04/09 06:00 [medline]
AID - 10.3760/cma.j.issn.2095-4352.2014.07.012 [doi]
PST - ppublish
SO  - Zhonghua Wei Zhong Bing Ji Jiu Yi Xue. 2014 Jul;26(7):503-7. doi: 
      10.3760/cma.j.issn.2095-4352.2014.07.012.