PMID- 25027946
OWN - NLM
STAT- MEDLINE
DCOM- 20150928
LR  - 20211021
IS  - 1434-4726 (Electronic)
IS  - 0937-4477 (Linking)
VI  - 272
IP  - 5
DP  - 2015 May
TI  - Establishment and characterization of an experimental mouse model of allergic 
      rhinitis.
PG  - 1149-55
LID - 10.1007/s00405-014-3176-2 [doi]
AB  - Allergic rhinitis (AR) is a common worldwide disease. Animal studies on AR were 
      adopted in various investigations. However, animal studies simply aimed at 
      establishing an animal model for AR have been seldom seen. The purpose of this 
      study was to introduce an easy-to-establish experimental mouse model of AR. To 
      develop a mouse model of AR, 38 Balb/c mice were randomly assigned to two groups. 
      Mice in the study group were sensitized by intraperitoneal (IP) injection of 
      ovalbumin (OVA) on day 1 and 6, followed by continuous inhalation (IH) of OVA 
      solution for 1 week (day 8-14) using a newly designed inhalation box. The control 
      group mice received sensitization of IP normal saline and IH sterilized distilled 
      water instead of OVA. Before and after sensitization, the frequencies of nasal 
      symptoms (sneezing, nasal rubbing) were recorded and the serum levels of total 
      immunoglobulin E (IgE) were evaluated using ELISA. Finally, the murine nasal 
      mucosal tissues were stained by Giemsa solution to estimate the degree of mast 
      cell infiltration. After sensitization by IP and IH OVA, the study group showed 
      significant phenotypic changes including increased sneezing and rubbing. 
      Pathological and cytological findings also confirmed significant elevated serum 
      total IgE titer and local mast cell infiltration in the study group 
      statistically. We successfully developed a workable experimental animal model for 
      AR that was more easily sensitized using our new-designed inhalation box, with 
      less stress and more precisely to be observed.
FAU - Ko, Ming-Tse
AU  - Ko MT
AD  - Graduate Institute of Clinical Medical Sciences, Chang Gung University College of 
      Medicine, Kaohsiung, Taiwan.
FAU - Huang, Shun-Chen
AU  - Huang SC
FAU - Kang, Hong-Yo
AU  - Kang HY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140716
PL  - Germany
TA  - Eur Arch Otorhinolaryngol
JT  - European archives of oto-rhino-laryngology : official journal of the European 
      Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the 
      German Society for Oto-Rhino-Laryngology - Head and Neck Surgery
JID - 9002937
RN  - 0 (Allergens)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Allergens/*administration & dosage
MH  - Animals
MH  - Disease Models, Animal
MH  - Drug Administration Routes
MH  - Immunoglobulin E/blood
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Nasal Mucosa/*pathology
MH  - Ovalbumin/*administration & dosage
MH  - *Rhinitis, Allergic/diagnosis/etiology/physiopathology
MH  - Sneezing/drug effects
EDAT- 2014/07/17 06:00
MHDA- 2015/09/29 06:00
CRDT- 2014/07/17 06:00
PHST- 2014/05/21 00:00 [received]
PHST- 2014/06/19 00:00 [accepted]
PHST- 2014/07/17 06:00 [entrez]
PHST- 2014/07/17 06:00 [pubmed]
PHST- 2015/09/29 06:00 [medline]
AID - 10.1007/s00405-014-3176-2 [doi]
PST - ppublish
SO  - Eur Arch Otorhinolaryngol. 2015 May;272(5):1149-55. doi: 
      10.1007/s00405-014-3176-2. Epub 2014 Jul 16.