PMID- 25028510
OWN - NLM
STAT- MEDLINE
DCOM- 20150122
LR  - 20211021
IS  - 1083-351X (Electronic)
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 289
IP  - 35
DP  - 2014 Aug 29
TI  - Peptide-dependent conformational fluctuation determines the stability of the 
      human leukocyte antigen class I complex.
PG  - 24680-90
LID - 10.1074/jbc.M114.566174 [doi]
AB  - In immune-mediated control of pathogens, human leukocyte antigen (HLA) class I 
      presents various antigenic peptides to CD8(+) T-cells. Long-lived peptide 
      presentation is important for efficient antigen-specific T-cell activation. 
      Presentation time depends on the peptide sequence and the stability of the 
      peptide-HLA complex (pHLA). However, the determinant of peptide-dependent pHLA 
      stability remains elusive. Here, to reveal the pHLA stabilization mechanism, we 
      examined the crystal structures of an HLA class I allomorph in complex with 
      HIV-derived peptides and evaluated site-specific conformational fluctuations 
      using NMR. Although the crystal structures of various pHLAs were almost identical 
      independent of the peptides, fluctuation analyses identified a peptide-dependent 
      minor state that would be more tightly packed toward the peptide. The minor 
      population correlated well with the thermostability and cell surface presentation 
      of pHLA, indicating that this newly identified minor state is important for 
      stabilizing the pHLA and facilitating T-cell recognition.
CI  - (c) 2014 by The American Society for Biochemistry and Molecular Biology, Inc.
FAU - Yanaka, Saeko
AU  - Yanaka S
AD  - From the Department of Medical Genome Sciences, Graduate School of Frontier 
      Sciences, The University of Tokyo, Tokyo 277-8562, Japan.
FAU - Ueno, Takamasa
AU  - Ueno T
AD  - Center for AIDS Research, Kumamoto University, Kumamoto 860-0811, Japan, 
      International Research Center for Medical Sciences (IRCMS), Kumamoto University, 
      Kumamoto 860-0811, Japan.
FAU - Shi, Yi
AU  - Shi Y
AD  - Research Network of Immunity and Health, Beijing Institute of Life Science, 
      Chinese Academy of Sciences, Beijing 100101, China, CAS Key Laboratory of 
      Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese 
      Academy of Sciences, Beijing 100101, China.
FAU - Qi, Jianxun
AU  - Qi J
AD  - Research Network of Immunity and Health, Beijing Institute of Life Science, 
      Chinese Academy of Sciences, Beijing 100101, China, CAS Key Laboratory of 
      Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese 
      Academy of Sciences, Beijing 100101, China.
FAU - Gao, George F
AU  - Gao GF
AD  - Research Network of Immunity and Health, Beijing Institute of Life Science, 
      Chinese Academy of Sciences, Beijing 100101, China, CAS Key Laboratory of 
      Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese 
      Academy of Sciences, Beijing 100101, China.
FAU - Tsumoto, Kouhei
AU  - Tsumoto K
AD  - From the Department of Medical Genome Sciences, Graduate School of Frontier 
      Sciences, The University of Tokyo, Tokyo 277-8562, Japan, Department of 
      Bioengineering, Graduate School of Engineering, The University of Tokyo, Tokyo 
      108-8693, Japan, Medical Proteomics Laboratory, Institute of Medical Science, The 
      University of Tokyo, Tokyo 108-8693, Japan, and tsumoto@k.u-tokyo.ac.jp.
FAU - Sugase, Kenji
AU  - Sugase K
AD  - Bioorganic Research Institute, Suntory Foundation for Life Sciences, Osaka 
      618-8503, Japan sugase@sunbor.or.jp.
LA  - eng
SI  - PDB/1A1N
SI  - PDB/1A9B
SI  - PDB/1A9E
SI  - PDB/1CG9
SI  - PDB/1XH3
SI  - PDB/1ZHK
SI  - PDB/1ZSD
SI  - PDB/2AXG
SI  - PDB/2CIK
SI  - PDB/2FYY
SI  - PDB/2H6P
SI  - PDB/2NX5
SI  - PDB/3LKN
SI  - PDB/3LKO
SI  - PDB/3LKP
SI  - PDB/3LKQ
SI  - PDB/3LKR
SI  - PDB/3LKS
SI  - PDB/3MV7
SI  - PDB/3MV8
SI  - PDB/3MV9
SI  - PDB/4LNR
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140715
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Peptides)
SB  - IM
MH  - Amino Acid Sequence
MH  - Crystallography, X-Ray
MH  - Histocompatibility Antigens Class I/*chemistry
MH  - Humans
MH  - Models, Molecular
MH  - Peptides/*chemistry
MH  - Protein Conformation
PMC - PMC4148890
OTO - NOTNLM
OT  - Major Histocompatibility Complex (MHC)
OT  - Nuclear Magnetic Resonance (NMR)
OT  - Protein Dynamic
OT  - Protein Stability
OT  - Thermodynamics
EDAT- 2014/07/17 06:00
MHDA- 2015/01/23 06:00
PMCR- 2015/08/29
CRDT- 2014/07/17 06:00
PHST- 2014/07/17 06:00 [entrez]
PHST- 2014/07/17 06:00 [pubmed]
PHST- 2015/01/23 06:00 [medline]
PHST- 2015/08/29 00:00 [pmc-release]
AID - S0021-9258(20)31998-0 [pii]
AID - M114.566174 [pii]
AID - 10.1074/jbc.M114.566174 [doi]
PST - ppublish
SO  - J Biol Chem. 2014 Aug 29;289(35):24680-90. doi: 10.1074/jbc.M114.566174. Epub 
      2014 Jul 15.