PMID- 25030435 OWN - NLM STAT- MEDLINE DCOM- 20150601 LR - 20140923 IS - 0028-2685 (Print) IS - 0028-2685 (Linking) VI - 61 IP - 5 DP - 2014 TI - RNA interference-mediated knockdown of brain-derived neurotrophic factor (BDNF) promotes cell cycle arrest and apoptosis in B-cell lymphoma cells. PG - 523-32 LID - 10.4149/neo_2014_064 [doi] AB - Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophin superfamily that has been reported to be involved in a number of neurological and psychological situations. Recently, high expression level of BDNF is observed in diverse human malignancies, delineating a role of BDNF in tumorigenesis. Nevertheless, its effect on B-cell lymphoma remains unclear. In this study, RNA interference technology mediated by short hairpin RNA (shRNA) was performed to inhibit endogenous BDNF expression in B-cell lymphoma cells. Results showed that knockdown of BDNF reduced cell growth and proliferation of Raji and Ramos cells. Furthermore, down-regulation of BDNF induced a cell cycle arrest at G0/G1 phase in Raji cells, and consequently led to cell apoptosis in vitro. Meanwhile, down-regulation of Bcl-2 and up-regulation of Bax, activated caspase-3 and caspase-9 and cleaved poly (ADP-ribose) polymerase (PARP) were observed in Raji cells when endogenous BDNF was inhibited. Besides, we also found that suppression of BDNF in Raji cells increased their sensitivity to chemotherapeutic drug, 5-Fluorouracil (5-FU). Our research provides a promising therapeutic strategy for human B-cell lymphoma by targeting BDNF. FAU - Xia, D AU - Xia D FAU - Li, W AU - Li W FAU - Zhang, L AU - Zhang L FAU - Qian, H AU - Qian H FAU - Yao, S AU - Yao S FAU - Qi, X AU - Qi X LA - eng PT - Journal Article PL - Slovakia TA - Neoplasma JT - Neoplasma JID - 0377266 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (RNA, Small Interfering) RN - U3P01618RT (Fluorouracil) SB - IM MH - *Apoptosis MH - Brain-Derived Neurotrophic Factor/*antagonists & inhibitors MH - Cell Cycle Checkpoints MH - Cell Line, Tumor MH - Fluorouracil/pharmacology MH - Humans MH - Lymphoma, B-Cell/pathology/*therapy MH - *RNA Interference MH - RNA, Small Interfering/genetics EDAT- 2014/07/18 06:00 MHDA- 2015/06/02 06:00 CRDT- 2014/07/18 06:00 PHST- 2014/07/18 06:00 [entrez] PHST- 2014/07/18 06:00 [pubmed] PHST- 2015/06/02 06:00 [medline] AID - 10.4149/neo_2014_064 [doi] PST - ppublish SO - Neoplasma. 2014;61(5):523-32. doi: 10.4149/neo_2014_064.