PMID- 25031400
OWN - NLM
STAT- MEDLINE
DCOM- 20140905
LR  - 20231213
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 34
IP  - 29
DP  - 2014 Jul 16
TI  - CREB SUMOylation by the E3 ligase PIAS1 enhances spatial memory.
PG  - 9574-89
LID - 10.1523/JNEUROSCI.4302-13.2014 [doi]
AB  - cAMP-responsive element binding protein (CREB) phosphorylation and signaling 
      plays an important role in long-term memory formation, but other 
      posttranslational modifications of CREB are less known. Here, we found that 
      CREB1Delta, the short isoform of CREB, could be sumoylated by the small 
      ubiquitin-like modifier (SUMO) E3 ligase protein inhibitor of activated STAT1 
      (PIAS1) at Lys271 and Lys290 and PIAS1 SUMOylation of CREB1Delta increased the 
      expression level of CREB1Delta. CREB1Delta could also be sumoylated by other PIAS family 
      proteins, but not by the E3 ligases RanBP2 and Pc2 or by the E2 ligase Ubc9. 
      Furthermore, water maze training increased the level of endogenous CREB 
      SUMOylation in rat CA1 neurons determined by in vitro SUMOylation assay, but this 
      effect was not observed in other brain areas. Moreover, transduction of 
      Lenti-CREBWT to rat CA1 area facilitated, whereas transduction of Lenti-CREB 
      double sumo-mutant (CREBK271RK290R) impaired, spatial learning and memory 
      performance. Transduction of Lenti-CREBWT-SUMO1 fusion vector to rat CA1 area 
      showed a more significant effect in enhancing spatial learning and memory and 
      CREB SUMOylation. Lenti-CREBWT transduction increased, whereas 
      Lenti-CREBK271RK290R transduction decreased, CREB DNA binding to the 
      brain-derived neurotrophic factor (bdnf) promoter and decreased bdnf mRNA 
      expression. Knock-down of PIAS1 expression in CA1 area by PIAS1 siRNA 
      transfection impaired spatial learning and memory and decreased endogenous CREB 
      SUMOylation. In addition, CREB SUMOylation was CREB phosphorylation dependent and 
      lasted longer. Therefore, CREB phosphorylation may be responsible for signal 
      transduction during the early phase of long-term memory formation, whereas CREB 
      SUMOylation sustains long-term memory.
CI  - Copyright (c) 2014 the authors 0270-6474/14/349574-16$15.00/0.
FAU - Chen, Yan-Chu
AU  - Chen YC
AD  - Graduate Institute of Life Sciences, National Defense Medical Center, and.
FAU - Hsu, Wei-Lun
AU  - Hsu WL
AD  - Institute of Biomedical Sciences, Academia Sinica, Taipei 115, Taiwan, Republic 
      of China.
FAU - Ma, Yun-Li
AU  - Ma YL
AD  - Institute of Biomedical Sciences, Academia Sinica, Taipei 115, Taiwan, Republic 
      of China.
FAU - Tai, Derek J C
AU  - Tai DJ
AD  - Institute of Biomedical Sciences, Academia Sinica, Taipei 115, Taiwan, Republic 
      of China.
FAU - Lee, Eminy H Y
AU  - Lee EH
AD  - Graduate Institute of Life Sciences, National Defense Medical Center, and 
      Institute of Biomedical Sciences, Academia Sinica, Taipei 115, Taiwan, Republic 
      of China eminy@gate.sinica.edu.tw.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Creb1 protein, rat)
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (Protein Inhibitors of Activated STAT)
RN  - 0 (Protein Isoforms)
RN  - 0 (enhanced green fluorescent protein)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cyclic AMP Response Element-Binding Protein/genetics/*metabolism
MH  - Gene Expression Regulation/drug effects/genetics
MH  - Green Fluorescent Proteins/genetics/metabolism
MH  - HEK293 Cells
MH  - Hippocampus/metabolism
MH  - Humans
MH  - Male
MH  - Maze Learning/drug effects/physiology
MH  - Memory/drug effects/*physiology
MH  - Protein Binding/genetics
MH  - Protein Inhibitors of Activated STAT/genetics/*metabolism
MH  - Protein Isoforms/genetics/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Signal Transduction/genetics
MH  - Space Perception/*physiology
MH  - Sumoylation/drug effects/genetics/*physiology
MH  - Time Factors
PMC - PMC6608321
OTO - NOTNLM
OT  - CREB
OT  - PIAS1
OT  - SUMOylation
OT  - phosphorylation
OT  - spatial learning and memory
EDAT- 2014/07/18 06:00
MHDA- 2014/09/06 06:00
PMCR- 2015/01/16
CRDT- 2014/07/18 06:00
PHST- 2014/07/18 06:00 [entrez]
PHST- 2014/07/18 06:00 [pubmed]
PHST- 2014/09/06 06:00 [medline]
PHST- 2015/01/16 00:00 [pmc-release]
AID - 34/29/9574 [pii]
AID - 4302-13 [pii]
AID - 10.1523/JNEUROSCI.4302-13.2014 [doi]
PST - ppublish
SO  - J Neurosci. 2014 Jul 16;34(29):9574-89. doi: 10.1523/JNEUROSCI.4302-13.2014.