PMID- 25031579
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20140717
LR  - 20240503
IS  - 1680-6433 (Print)
IS  - 2008-2177 (Electronic)
IS  - 1680-6433 (Linking)
VI  - 12
IP  - 5
DP  - 2014 May
TI  - Effect of saffron on rat sperm chromatin integrity.
PG  - 343-50
AB  - BACKGROUND: Currently, relation between reactive oxygen species (ROS) ROS 
      concentration and semen quality was indicated. Saffron has traditionally been not 
      only considered as a food additive but also as a medicinal herb, which has a good 
      antioxidant properties. OBJECTIVE: The aim of this study was to evaluate the 
      protection potency of saffron and vitamin E on sperm chromatin integrity. 
      MATERIALS AND METHODS: Thirty adult male Wistar rats divided equally into saffron 
      (100 mg/kg), vitamin E (100 mg/kg) and control (0.5cc distilled water /day) 
      groups. After 60 days, cauda epididymis dissected and sperm cells were used for 
      analysis of sperm chromatin packaging by chromomycin A3 (CMA3) staining, and 
      sperm chromatin susceptibility to acid denaturation by acridine orange (AO) 
      staining. RESULTS: The mean percentage of CMA3 positive sperm was significantly 
      decreased in saffron and vitamin E groups relative to control group (p<0.001). 
      Moreover, the AO staining results showed that the mean percentage of sperm with 
      DNA damage was significantly decreased in saffron and vitamin E groups as 
      compared with control group (p<0.001). CONCLUSION: Our results purposed that 
      saffron can protect sperm against DNA damage and chromatin anomalies.
FAU - Mardani, Mohammad
AU  - Mardani M
AD  - Department of Anatomical Sciences and Molecular Biology, School of Medicine, 
      Isfahan University of Medical Sciences, Isfahan, Iran.
FAU - Vaez, Ahmad
AU  - Vaez A
AD  - Department of Anatomical Sciences and Molecular Biology, School of Medicine, 
      Isfahan University of Medical Sciences, Isfahan, Iran.
FAU - Razavi, Shahnaz
AU  - Razavi S
AD  - Department of Anatomical Sciences and Molecular Biology, School of Medicine, 
      Isfahan University of Medical Sciences, Isfahan, Iran.
LA  - eng
PT  - Journal Article
PL  - Iran
TA  - Iran J Reprod Med
JT  - Iranian journal of reproductive medicine
JID - 101463749
PMC - PMC4094660
OTO - NOTNLM
OT  - Antioxidants
OT  - DNA damage
OT  - Saffron
OT  - Sperm chromatin
OT  - Vitamin E
EDAT- 2014/07/18 06:00
MHDA- 2014/07/18 06:01
PMCR- 2014/05/01
CRDT- 2014/07/18 06:00
PHST- 2013/08/19 00:00 [received]
PHST- 2014/01/08 00:00 [revised]
PHST- 2014/01/13 00:00 [accepted]
PHST- 2014/07/18 06:00 [entrez]
PHST- 2014/07/18 06:00 [pubmed]
PHST- 2014/07/18 06:01 [medline]
PHST- 2014/05/01 00:00 [pmc-release]
AID - ijrm-12-343 [pii]
PST - ppublish
SO  - Iran J Reprod Med. 2014 May;12(5):343-50.