PMID- 25034259
OWN - NLM
STAT- MEDLINE
DCOM- 20151102
LR  - 20211021
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 4
DP  - 2014 Jul 17
TI  - Determination of the ruminant origin of bone particles using fluorescence in situ 
      hybridization (FISH).
PG  - 5730
LID - 10.1038/srep05730 [doi]
LID - 5730
AB  - Molecular biology techniques such as PCR constitute powerful tools for the 
      determination of the taxonomic origin of bones. DNA degradation and contamination 
      by exogenous DNA, however, jeopardise bone identification. Despite the vast array 
      of techniques used to decontaminate bone fragments, the isolation and 
      determination of bone DNA content are still problematic. Within the framework of 
      the eradication of transmissible spongiform encephalopathies (including BSE, 
      commonly known as "mad cow disease"), a fluorescence in situ hybridization (FISH) 
      protocol was developed. Results from the described study showed that this method 
      can be applied directly to bones without a demineralisation step and that it 
      allows the identification of bovine and ruminant bones even after severe 
      processing. The results also showed that the method is independent of exogenous 
      contamination and that it is therefore entirely appropriate for this application.
FAU - Lecrenier, M C
AU  - Lecrenier MC
AD  - 1] Walloon Agricultural Research Centre CRA-W, Chaussee de Namur, 24, B-5030 
      Gembloux, Belgium [2] Faculty of Veterinary Medicine, University of Liege, 
      Boulevard de Colonster 20, B-42 Sart-Tilman, B-4000 Liege, Belgium [3].
FAU - Ledoux, Q
AU  - Ledoux Q
AD  - 1] Walloon Agricultural Research Centre CRA-W, Chaussee de Namur, 24, B-5030 
      Gembloux, Belgium [2].
FAU - Berben, G
AU  - Berben G
AD  - Walloon Agricultural Research Centre CRA-W, Chaussee de Namur, 24, B-5030 
      Gembloux, Belgium.
FAU - Fumiere, O
AU  - Fumiere O
AD  - Walloon Agricultural Research Centre CRA-W, Chaussee de Namur, 24, B-5030 
      Gembloux, Belgium.
FAU - Saegerman, C
AU  - Saegerman C
AD  - Faculty of Veterinary Medicine, University of Liege, Boulevard de Colonster 20, 
      B-42 Sart-Tilman, B-4000 Liege, Belgium.
FAU - Baeten, V
AU  - Baeten V
AD  - Walloon Agricultural Research Centre CRA-W, Chaussee de Namur, 24, B-5030 
      Gembloux, Belgium.
FAU - Veys, P
AU  - Veys P
AD  - Walloon Agricultural Research Centre CRA-W, Chaussee de Namur, 24, B-5030 
      Gembloux, Belgium.
LA  - eng
PT  - Journal Article
DEP - 20140717
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (DNA Probes)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - *Animal Feed
MH  - Animals
MH  - Bone and Bones/*chemistry
MH  - Cattle
MH  - DNA/*genetics
MH  - DNA Probes/genetics
MH  - In Situ Hybridization, Fluorescence
PMC - PMC4103152
EDAT- 2014/07/19 06:00
MHDA- 2015/11/03 06:00
PMCR- 2014/07/17
CRDT- 2014/07/19 06:00
PHST- 2013/12/18 00:00 [received]
PHST- 2014/06/30 00:00 [accepted]
PHST- 2014/07/19 06:00 [entrez]
PHST- 2014/07/19 06:00 [pubmed]
PHST- 2015/11/03 06:00 [medline]
PHST- 2014/07/17 00:00 [pmc-release]
AID - srep05730 [pii]
AID - 10.1038/srep05730 [doi]
PST - epublish
SO  - Sci Rep. 2014 Jul 17;4:5730. doi: 10.1038/srep05730.