PMID- 25037215
OWN - NLM
STAT- MEDLINE
DCOM- 20150720
LR  - 20231213
IS  - 1532-2548 (Electronic)
IS  - 0032-0889 (Print)
IS  - 0032-0889 (Linking)
VI  - 166
IP  - 3
DP  - 2014 Nov
TI  - Tandem amplification of a chromosomal segment harboring 
      5-enolpyruvylshikimate-3-phosphate synthase locus confers glyphosate resistance 
      in Kochia scoparia.
PG  - 1200-7
LID - 10.1104/pp.114.242826 [doi]
AB  - Recent rapid evolution and spread of resistance to the most extensively used 
      herbicide, glyphosate, is a major threat to global crop production. Genetic 
      mechanisms by which weeds evolve resistance to herbicides largely determine the 
      level of resistance and the rate of evolution of resistance. In a previous study, 
      we determined that glyphosate resistance in Kochia scoparia is due to the 
      amplification of the 5-Enolpyruvylshikimate-3-Phosphate Synthase (EPSPS) gene, 
      the enzyme target of glyphosate. Here, we investigated the genomic organization 
      of the amplified EPSPS copies using fluorescence in situ hybridization (FISH) and 
      extended DNA fiber (Fiber FISH) on K. scoparia chromosomes. In both 
      glyphosate-resistant K. scoparia populations tested (GR1 and GR2), FISH results 
      displayed a single and prominent hybridization site of the EPSPS gene localized 
      on the distal end of one pair of homologous metaphase chromosomes compared with a 
      faint hybridization site in glyphosate-susceptible samples (GS1 and GS2). Fiber 
      FISH displayed 10 copies of the EPSPS gene (approximately 5 kb) arranged in 
      tandem configuration approximately 40 to 70 kb apart, with one copy in an 
      inverted orientation in GR2. In agreement with FISH results, segregation of EPSPS 
      copies followed single-locus inheritance in GR1 population. This is the first 
      report of tandem target gene amplification conferring field-evolved herbicide 
      resistance in weed populations.
CI  - (c) 2014 American Society of Plant Biologists. All Rights Reserved.
FAU - Jugulam, Mithila
AU  - Jugulam M
AD  - Departments of Agronomy (M.J., K.N., A.S.G., V.K.V.) and Plant Pathology 
      (D.-H.K., T.D., B.F., S.S., B.S.G.), Kansas State University, Manhattan, Kansas 
      66506;Department of Bioagricultural Sciences and Pest Management, Colorado State 
      University, Fort Collins, Colorado 80523 (A.W., P.W.); andKansas State University 
      Agricultural Research Center, Hays, Kansas 67601 (P.W.S.) mithila@ksu.edu.
FAU - Niehues, Kindsey
AU  - Niehues K
AD  - Departments of Agronomy (M.J., K.N., A.S.G., V.K.V.) and Plant Pathology 
      (D.-H.K., T.D., B.F., S.S., B.S.G.), Kansas State University, Manhattan, Kansas 
      66506;Department of Bioagricultural Sciences and Pest Management, Colorado State 
      University, Fort Collins, Colorado 80523 (A.W., P.W.); andKansas State University 
      Agricultural Research Center, Hays, Kansas 67601 (P.W.S.).
FAU - Godar, Amar S
AU  - Godar AS
AUID- ORCID: 0000-0001-9980-6562
AD  - Departments of Agronomy (M.J., K.N., A.S.G., V.K.V.) and Plant Pathology 
      (D.-H.K., T.D., B.F., S.S., B.S.G.), Kansas State University, Manhattan, Kansas 
      66506;Department of Bioagricultural Sciences and Pest Management, Colorado State 
      University, Fort Collins, Colorado 80523 (A.W., P.W.); andKansas State University 
      Agricultural Research Center, Hays, Kansas 67601 (P.W.S.).
FAU - Koo, Dal-Hoe
AU  - Koo DH
AD  - Departments of Agronomy (M.J., K.N., A.S.G., V.K.V.) and Plant Pathology 
      (D.-H.K., T.D., B.F., S.S., B.S.G.), Kansas State University, Manhattan, Kansas 
      66506;Department of Bioagricultural Sciences and Pest Management, Colorado State 
      University, Fort Collins, Colorado 80523 (A.W., P.W.); andKansas State University 
      Agricultural Research Center, Hays, Kansas 67601 (P.W.S.).
FAU - Danilova, Tatiana
AU  - Danilova T
AD  - Departments of Agronomy (M.J., K.N., A.S.G., V.K.V.) and Plant Pathology 
      (D.-H.K., T.D., B.F., S.S., B.S.G.), Kansas State University, Manhattan, Kansas 
      66506;Department of Bioagricultural Sciences and Pest Management, Colorado State 
      University, Fort Collins, Colorado 80523 (A.W., P.W.); andKansas State University 
      Agricultural Research Center, Hays, Kansas 67601 (P.W.S.).
FAU - Friebe, Bernd
AU  - Friebe B
AD  - Departments of Agronomy (M.J., K.N., A.S.G., V.K.V.) and Plant Pathology 
      (D.-H.K., T.D., B.F., S.S., B.S.G.), Kansas State University, Manhattan, Kansas 
      66506;Department of Bioagricultural Sciences and Pest Management, Colorado State 
      University, Fort Collins, Colorado 80523 (A.W., P.W.); andKansas State University 
      Agricultural Research Center, Hays, Kansas 67601 (P.W.S.).
FAU - Sehgal, Sunish
AU  - Sehgal S
AD  - Departments of Agronomy (M.J., K.N., A.S.G., V.K.V.) and Plant Pathology 
      (D.-H.K., T.D., B.F., S.S., B.S.G.), Kansas State University, Manhattan, Kansas 
      66506;Department of Bioagricultural Sciences and Pest Management, Colorado State 
      University, Fort Collins, Colorado 80523 (A.W., P.W.); andKansas State University 
      Agricultural Research Center, Hays, Kansas 67601 (P.W.S.).
FAU - Varanasi, Vijay K
AU  - Varanasi VK
AD  - Departments of Agronomy (M.J., K.N., A.S.G., V.K.V.) and Plant Pathology 
      (D.-H.K., T.D., B.F., S.S., B.S.G.), Kansas State University, Manhattan, Kansas 
      66506;Department of Bioagricultural Sciences and Pest Management, Colorado State 
      University, Fort Collins, Colorado 80523 (A.W., P.W.); andKansas State University 
      Agricultural Research Center, Hays, Kansas 67601 (P.W.S.).
FAU - Wiersma, Andrew
AU  - Wiersma A
AD  - Departments of Agronomy (M.J., K.N., A.S.G., V.K.V.) and Plant Pathology 
      (D.-H.K., T.D., B.F., S.S., B.S.G.), Kansas State University, Manhattan, Kansas 
      66506;Department of Bioagricultural Sciences and Pest Management, Colorado State 
      University, Fort Collins, Colorado 80523 (A.W., P.W.); andKansas State University 
      Agricultural Research Center, Hays, Kansas 67601 (P.W.S.).
FAU - Westra, Philip
AU  - Westra P
AD  - Departments of Agronomy (M.J., K.N., A.S.G., V.K.V.) and Plant Pathology 
      (D.-H.K., T.D., B.F., S.S., B.S.G.), Kansas State University, Manhattan, Kansas 
      66506;Department of Bioagricultural Sciences and Pest Management, Colorado State 
      University, Fort Collins, Colorado 80523 (A.W., P.W.); andKansas State University 
      Agricultural Research Center, Hays, Kansas 67601 (P.W.S.).
FAU - Stahlman, Phillip W
AU  - Stahlman PW
AD  - Departments of Agronomy (M.J., K.N., A.S.G., V.K.V.) and Plant Pathology 
      (D.-H.K., T.D., B.F., S.S., B.S.G.), Kansas State University, Manhattan, Kansas 
      66506;Department of Bioagricultural Sciences and Pest Management, Colorado State 
      University, Fort Collins, Colorado 80523 (A.W., P.W.); andKansas State University 
      Agricultural Research Center, Hays, Kansas 67601 (P.W.S.).
FAU - Gill, Bikram S
AU  - Gill BS
AD  - Departments of Agronomy (M.J., K.N., A.S.G., V.K.V.) and Plant Pathology 
      (D.-H.K., T.D., B.F., S.S., B.S.G.), Kansas State University, Manhattan, Kansas 
      66506;Department of Bioagricultural Sciences and Pest Management, Colorado State 
      University, Fort Collins, Colorado 80523 (A.W., P.W.); andKansas State University 
      Agricultural Research Center, Hays, Kansas 67601 (P.W.S.).
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20140718
PL  - United States
TA  - Plant Physiol
JT  - Plant physiology
JID - 0401224
RN  - 0 (Herbicides)
RN  - EC 2.5.1.19 (3-Phosphoshikimate 1-Carboxyvinyltransferase)
RN  - TE7660XO1C (Glycine)
SB  - IM
MH  - 3-Phosphoshikimate 1-Carboxyvinyltransferase/*genetics/metabolism
MH  - Bassia scoparia/*enzymology/genetics
MH  - Biological Evolution
MH  - Chromosome Mapping
MH  - Gene Amplification
MH  - Gene Expression Regulation, Plant/drug effects
MH  - Glycine/*analogs & derivatives/pharmacology
MH  - *Herbicide Resistance
MH  - Herbicides/*pharmacology
MH  - In Situ Hybridization, Fluorescence
MH  - Models, Biological
MH  - Glyphosate
PMC - PMC4226373
EDAT- 2014/07/20 06:00
MHDA- 2015/07/21 06:00
PMCR- 2014/07/18
CRDT- 2014/07/20 06:00
PHST- 2014/07/20 06:00 [entrez]
PHST- 2014/07/20 06:00 [pubmed]
PHST- 2015/07/21 06:00 [medline]
PHST- 2014/07/18 00:00 [pmc-release]
AID - pp.114.242826 [pii]
AID - 242826 [pii]
AID - 10.1104/pp.114.242826 [doi]
PST - ppublish
SO  - Plant Physiol. 2014 Nov;166(3):1200-7. doi: 10.1104/pp.114.242826. Epub 2014 Jul 
      18.