PMID- 25037435 OWN - NLM STAT- MEDLINE DCOM- 20151005 LR - 20161125 IS - 1879-1344 (Electronic) IS - 0144-8617 (Linking) VI - 111 DP - 2014 Oct 13 TI - Purification and antioxidant activities of intracellular zinc polysaccharides from Pleurotus cornucopiae SS-03. PG - 947-54 LID - S0144-8617(14)00431-7 [pii] LID - 10.1016/j.carbpol.2014.04.074 [doi] AB - Intracellular zinc polysaccharides (IZPS) from Pleurotus cornucopiae SS-03 were extracted and purified, and three subfractions (IZPS-1, IZPS-2, and IZPS-3) were separated by DEAE-52 cellulose anion-exchange column chromatography. They showed certain scavenging effects on superoxide anion (O2(*-)) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, and positive rising of reducing power in vitro. All the subfractions were found upregulated the enzyme activities of superoxide dismutase (SOD), GSH peroxide (GSH-Px) and catalase (CAT) significantly, decreased the contents of malondialdehyde (MDA) and lipid peroxidation (LPO) remarkably in vivo. In addition, five monosaccharides components, including rhamnose, xylose, mannose, glucose, and galactose with the molar ratio of 1.65:0.05:1:1.50:1.07 and contents of 29.25%, 0.78%, 19.45%, 29.84%, and 20.67%, were investigated by gas chromatography. CI - Copyright (c) 2014 Elsevier Ltd. All rights reserved. FAU - Zhang, Jianjun AU - Zhang J AD - College of Life Science, Shandong Agricultural University, Taian, Shandong 271018, PR China. FAU - Ma, Zhao AU - Ma Z AD - College of Life Science, Shandong Agricultural University, Taian, Shandong 271018, PR China. FAU - Zheng, Lan AU - Zheng L AD - College of Life Science, Shandong Agricultural University, Taian, Shandong 271018, PR China. FAU - Zhai, Guoyin AU - Zhai G AD - College of Life Science, Shandong Agricultural University, Taian, Shandong 271018, PR China. FAU - Wang, Liqin AU - Wang L AD - College of Life Science, Shandong Agricultural University, Taian, Shandong 271018, PR China. FAU - Jia, Mengshi AU - Jia M AD - The Second High School of Taian, Taian, Shandong 271000, PR China. FAU - Jia, Le AU - Jia L AD - College of Life Science, Shandong Agricultural University, Taian, Shandong 271018, PR China. Electronic address: ourlaboratory@163.com. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20140529 PL - England TA - Carbohydr Polym JT - Carbohydrate polymers JID - 8307156 RN - 0 (Antioxidants) RN - 0 (Biphenyl Compounds) RN - 0 (Picrates) RN - 0 (Polysaccharides) RN - 11062-77-4 (Superoxides) RN - 4Y8F71G49Q (Malondialdehyde) RN - DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl) RN - EC 1.11.1.6 (Catalase) RN - EC 1.15.1.1 (Superoxide Dismutase) RN - J41CSQ7QDS (Zinc) SB - IM MH - Animals MH - Antioxidants/administration & dosage/*chemistry/isolation & purification/*pharmacology MH - Biphenyl Compounds/metabolism MH - Catalase/metabolism MH - Lipid Peroxidation/drug effects MH - Male MH - Malondialdehyde/metabolism MH - Mice MH - Picrates/metabolism MH - Pleurotus/*chemistry MH - Polysaccharides/administration & dosage/*chemistry/isolation & purification/*pharmacology MH - Superoxide Dismutase/metabolism MH - Superoxides/metabolism MH - Zinc/*analysis/pharmacology OTO - NOTNLM OT - Antioxidant activities OT - Pleurotus cornucopiae SS-03 OT - Polysaccharides OT - Purification OT - Zinc EDAT- 2014/07/20 06:00 MHDA- 2015/10/06 06:00 CRDT- 2014/07/20 06:00 PHST- 2014/01/17 00:00 [received] PHST- 2014/04/08 00:00 [revised] PHST- 2014/04/13 00:00 [accepted] PHST- 2014/07/20 06:00 [entrez] PHST- 2014/07/20 06:00 [pubmed] PHST- 2015/10/06 06:00 [medline] AID - S0144-8617(14)00431-7 [pii] AID - 10.1016/j.carbpol.2014.04.074 [doi] PST - ppublish SO - Carbohydr Polym. 2014 Oct 13;111:947-54. doi: 10.1016/j.carbpol.2014.04.074. Epub 2014 May 29.