PMID- 25037720
OWN - NLM
STAT- MEDLINE
DCOM- 20141222
LR  - 20141017
IS  - 1432-8798 (Electronic)
IS  - 0304-8608 (Linking)
VI  - 159
IP  - 11
DP  - 2014 Nov
TI  - Characterisation of novel linear antigen epitopes on North American-type porcine 
      reproductive and respiratory syndrome virus M protein.
PG  - 3021-8
LID - 10.1007/s00705-014-2174-4 [doi]
AB  - The M protein, encoded by the porcine reproductive and respiratory syndrome virus 
      (PRRSV) ORF6 gene, is considered to be one of the most conserved PRRSV proteins. 
      In recent decades, highly specific monoclonal antibodies (Mabs) have been 
      exploited to provide reliable diagnoses for many diseases. In this study, two 
      different Mab clones targeting the linear epitopes on the PRRSV M protein were 
      generated and characterized. Both Mabs showed binding activity against the native 
      PRRSV virion and recombinant M protein when analyzed by immunofluorescence assay 
      (IFA) and Western blot. The targeted epitope of each Mab was mapped by serial 
      truncation of the M protein to generate overlapping fragments. Fine epitope 
      mapping was then performed using a panel of expressed polypeptides. The 
      polypeptide sequences of the two epitopes recognized by Mabs 1C8 and 3F7 were 
      (3)SSLD(6) and (155)VLGGRKAVK(163), respectively, with the former being a newly 
      identified epitope on the M protein. In both cases, these two epitopes were 
      finely mapped for the first time. Alignments of Mab epitope sequences revealed 
      that the two epitopes on the M protein were highly conserved between the North 
      American-type strains. These Mabs, along with their mapped epitopes, are useful 
      for the development of diagnostic and research tools, including 
      immunofluorescence, ELISA and Western blot.
FAU - Wang, Qian
AU  - Wang Q
AD  - Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, 
      College of Veterinary Medicine and State Key Laboratory of Agribiotechnology, 
      China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian District, 
      Beijing, 100193, China.
FAU - Chen, Jiazeng
AU  - Chen J
FAU - Peng, Jinmei
AU  - Peng J
FAU - An, Tongqing
AU  - An T
FAU - Leng, Chaoliang
AU  - Leng C
FAU - Sun, Yan
AU  - Sun Y
FAU - Guo, Xin
AU  - Guo X
FAU - Ge, Xinna
AU  - Ge X
FAU - Tian, Zhijun
AU  - Tian Z
FAU - Yang, Hanchun
AU  - Yang H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140719
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antibodies, Viral)
RN  - 0 (M protein, porcine reproductive and respiratory syndrome virus)
RN  - 0 (Viral Matrix Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies, Monoclonal/immunology
MH  - Antibodies, Viral/immunology
MH  - *Epitope Mapping
MH  - Female
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Molecular Sequence Data
MH  - North America
MH  - Porcine Reproductive and Respiratory Syndrome/*virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/chemistry/genetics/*immunology
MH  - Swine
MH  - Viral Matrix Proteins/*chemistry/genetics/*immunology
EDAT- 2014/07/20 06:00
MHDA- 2014/12/23 06:00
CRDT- 2014/07/20 06:00
PHST- 2014/03/18 00:00 [received]
PHST- 2014/06/30 00:00 [accepted]
PHST- 2014/07/20 06:00 [entrez]
PHST- 2014/07/20 06:00 [pubmed]
PHST- 2014/12/23 06:00 [medline]
AID - 10.1007/s00705-014-2174-4 [doi]
PST - ppublish
SO  - Arch Virol. 2014 Nov;159(11):3021-8. doi: 10.1007/s00705-014-2174-4. Epub 2014 
      Jul 19.