PMID- 25038658
OWN - NLM
STAT- MEDLINE
DCOM- 20151201
LR  - 20140721
IS  - 1873-7072 (Electronic)
IS  - 0308-8146 (Linking)
VI  - 165
DP  - 2014 Dec 15
TI  - Identification of a NFkappaB inhibitory peptide from tryptic beta-casein hydrolysate.
PG  - 129-33
LID - S0308-8146(14)00791-2 [pii]
LID - 10.1016/j.foodchem.2014.05.075 [doi]
AB  - Several bioactive peptides are encrypted within the sequence of major milk 
      proteins, requiring enzymatic proteolysis for release and activation. The present 
      study aimed at the identification of potential anti-inflammatory activities in 
      tryptic hydrolysates of bovine beta-casein. Inflammatory processes involve in most 
      cases an activation of Nuclear factor Kappa-light-chain enhancer of activated B 
      cells (NFkappaB), which is a pro-inflammatory transcription factor of several genes. 
      Hence, a NFkappaB reporter cell line was established, and TNF-alpha mediated activation 
      of NFkappaB was used as a measurement. Bovine beta-casein (beta-CN) was hydrolysed by 
      trypsin and fractionated by ultrafiltration. Total proteolysate as well as the 
      fraction containing peptides between 1 and 5 kDa showed an inhibitory effect in 
      the cell-based assay, while the fraction containing molecules smaller than 1 kDa 
      did not. This anti-inflammatory effect was ascribed to a group of large, 
      hydrophobic peptides, which were identified using LC-MS. The main peptide was 
      synthesised and showed a significant anti-inflammatory effect in 
      HEK(nfkb-RE)-cells. Thus, for the first time, a casein-derived peptide having an 
      anti-inflammatory effect in vitro has been identified.
CI  - Copyright (c) 2014 Elsevier Ltd. All rights reserved.
FAU - Malinowski, J
AU  - Malinowski J
AD  - Department of Safety and Quality of Milk and Fish Products, Max Rubner-Institut 
      Kiel, Hermann-Weigmann-Strasse 1, 24103 Kiel, Germany.
FAU - Klempt, M
AU  - Klempt M
AD  - Department of Safety and Quality of Milk and Fish Products, Max Rubner-Institut 
      Kiel, Hermann-Weigmann-Strasse 1, 24103 Kiel, Germany. Electronic address: 
      martin.klempt@mri.bund.de.
FAU - Clawin-Radecker, I
AU  - Clawin-Radecker I
AD  - Department of Safety and Quality of Milk and Fish Products, Max Rubner-Institut 
      Kiel, Hermann-Weigmann-Strasse 1, 24103 Kiel, Germany.
FAU - Lorenzen, P Chr
AU  - Lorenzen PC
AD  - Department of Safety and Quality of Milk and Fish Products, Max Rubner-Institut 
      Kiel, Hermann-Weigmann-Strasse 1, 24103 Kiel, Germany.
FAU - Meisel, H
AU  - Meisel H
AD  - Department of Safety and Quality of Milk and Fish Products, Max Rubner-Institut 
      Kiel, Hermann-Weigmann-Strasse 1, 24103 Kiel, Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140523
PL  - England
TA  - Food Chem
JT  - Food chemistry
JID - 7702639
RN  - 0 (Caseins)
RN  - 0 (NF-kappa B)
RN  - 0 (Peptides)
RN  - 65072-00-6 (casein hydrolysate)
RN  - EC 3.4.21.4 (Trypsin)
SB  - IM
MH  - Animals
MH  - Caseins/*chemistry
MH  - Cattle
MH  - NF-kappa B/*chemistry/metabolism
MH  - Peptides/*pharmacology
MH  - Trypsin/metabolism
OTO - NOTNLM
OT  - Anti-inflammatory peptides
OT  - Bioactive peptides
OT  - Bovine beta-casein
OT  - Enzymatic hydrolysis
EDAT- 2014/07/21 06:00
MHDA- 2015/12/15 06:00
CRDT- 2014/07/21 06:00
PHST- 2014/01/13 00:00 [received]
PHST- 2014/04/08 00:00 [revised]
PHST- 2014/05/14 00:00 [accepted]
PHST- 2014/07/21 06:00 [entrez]
PHST- 2014/07/21 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
AID - S0308-8146(14)00791-2 [pii]
AID - 10.1016/j.foodchem.2014.05.075 [doi]
PST - ppublish
SO  - Food Chem. 2014 Dec 15;165:129-33. doi: 10.1016/j.foodchem.2014.05.075. Epub 2014 
      May 23.