PMID- 25038689 OWN - NLM STAT- MEDLINE DCOM- 20151201 LR - 20181202 IS - 1873-7072 (Electronic) IS - 0308-8146 (Linking) VI - 165 DP - 2014 Dec 15 TI - Automated analytical standard production with supercritical fluid chromatography for the quantification of bioactive C17-polyacetylenes: a case study on food processing waste. PG - 371-8 LID - S0308-8146(14)00809-7 [pii] LID - 10.1016/j.foodchem.2014.05.093 [doi] AB - Food processing enterprises produce enormous amounts of organic waste that contains valuable phytochemicals (e.g. C17-polyacetylenes). Knowledge on the phytochemicals content is a first step towards valorisation. Quantification of C17-polyacetylenes is however often hampered by the lack of commercially available standards or by tedious multistep in-house standard production procedures. In the current study, a new and straightforward supercritical fluid chromatography purification procedure is described for the simultaneous production of 2 analytical C17-polyacetylene standards. Respectively, 5 and 6 mg of falcarinol and falcarindiol were purified in 17 h on analytical scale. After confirming the identity and quality (97% purity) by Nuclear Magnetic Resonance, accurate mass-Mass Spectrometry (am-MS) and Photo Diode Array (PDA) detection the C17-polyacetylene standards were used for the analysis of industrial vegetable waste with Liquid Chromatography coupled to PDA and am-MS detection. Measurements showed varying concentrations of C17-polyacetylenes in the organic waste depending on its nature and origin. CI - Copyright (c) 2014 Elsevier Ltd. All rights reserved. FAU - Bijttebier, Sebastiaan AU - Bijttebier S AD - Flemish Institute for Technological Research (VITO), Business Unit Separation and Conversion Technology (SCT), Boeretang 200, 2400 Mol, Belgium; University of Antwerp, NatuRA, Universiteitsplein 1, 2610 Antwerp, Belgium. Electronic address: sebastiaan.bijttebier@vito.be. FAU - D'Hondt, Els AU - D'Hondt E AD - Flemish Institute for Technological Research (VITO), Business Unit Separation and Conversion Technology (SCT), Boeretang 200, 2400 Mol, Belgium. Electronic address: els.dhondt@vito.be. FAU - Noten, Bart AU - Noten B AD - Flemish Institute for Technological Research (VITO), Business Unit Separation and Conversion Technology (SCT), Boeretang 200, 2400 Mol, Belgium. Electronic address: bart.noten@vito.be. FAU - Hermans, Nina AU - Hermans N AD - University of Antwerp, NatuRA, Universiteitsplein 1, 2610 Antwerp, Belgium. Electronic address: nina.hermans@uantwerpen.be. FAU - Apers, Sandra AU - Apers S AD - University of Antwerp, NatuRA, Universiteitsplein 1, 2610 Antwerp, Belgium. Electronic address: sandra.apers@uantwerpen.be. FAU - Exarchou, Vassiliki AU - Exarchou V AD - University of Antwerp, NatuRA, Universiteitsplein 1, 2610 Antwerp, Belgium. Electronic address: vasiliki.exarchou@uantwerpen.be. FAU - Voorspoels, Stefan AU - Voorspoels S AD - Flemish Institute for Technological Research (VITO), Business Unit Separation and Conversion Technology (SCT), Boeretang 200, 2400 Mol, Belgium. Electronic address: stefan.voorspoels@vito.be. LA - eng PT - Journal Article DEP - 20140529 PL - England TA - Food Chem JT - Food chemistry JID - 7702639 RN - 25067-58-7 (Polyynes) SB - IM MH - Chromatography, Liquid/*methods MH - Chromatography, Supercritical Fluid/*methods MH - Food Handling/*methods MH - Mass Spectrometry/*methods MH - Polyynes/*analysis OTO - NOTNLM OT - C17-polyacetylenes OT - Carotenoids OT - Food processing waste OT - LC-PDA-am-MS OT - Purification OT - Semi-prep SFC EDAT- 2014/07/21 06:00 MHDA- 2015/12/15 06:00 CRDT- 2014/07/21 06:00 PHST- 2014/01/24 00:00 [received] PHST- 2014/04/30 00:00 [revised] PHST- 2014/05/16 00:00 [accepted] PHST- 2014/07/21 06:00 [entrez] PHST- 2014/07/21 06:00 [pubmed] PHST- 2015/12/15 06:00 [medline] AID - S0308-8146(14)00809-7 [pii] AID - 10.1016/j.foodchem.2014.05.093 [doi] PST - ppublish SO - Food Chem. 2014 Dec 15;165:371-8. doi: 10.1016/j.foodchem.2014.05.093. Epub 2014 May 29.