PMID- 25041640
OWN - NLM
STAT- MEDLINE
DCOM- 20150518
LR  - 20140904
IS  - 1574-6968 (Electronic)
IS  - 0378-1097 (Linking)
VI  - 358
IP  - 1
DP  - 2014 Sep
TI  - In situ identification of the synthrophic protein fermentative Coprothermobacter 
      spp. involved in the thermophilic anaerobic digestion process.
PG  - 55-63
LID - 10.1111/1574-6968.12528 [doi]
AB  - Thermophilic bacteria have recently attracted great attention because of their 
      potential application in improving different biochemical processes such as 
      anaerobic digestion of various substrates, wastewater treatment or hydrogen 
      production. In this study we report on the design of a specific 16S rRNA-targeted 
      oligonucleotide probe for detecting members of Coprothermobacter genus 
      characterized by a strong protease activity to degrade proteins and peptides. The 
      newly designed CTH485 probe and helper probes hCTH429 and hCTH439 were optimized 
      for use in fluorescence in situ hybridization (FISH) on thermophilic anaerobic 
      sludge samples. In situ probing revealed that thermo-adaptive mechanisms shaping 
      the 16S rRNA gene may affect the identification of thermophilic microorganisms. 
      The novel developed FISH probe extends the possibility to study the widespread 
      thermophilic syntrophic interaction of Coprothermobacter spp. with 
      hydrogenotrophic methanogenic archaea, whose establishment is a great benefit for 
      the whole anaerobic system.
CI  - (c) 2014 Federation of European Microbiological Societies. Published by John Wiley 
      & Sons Ltd. All rights reserved.
FAU - Gagliano, Maria Cristina
AU  - Gagliano MC
AD  - Water Research Institute, IRSA-CNR, Monterotondo, RM, Italy.
FAU - Braguglia, Camilla Maria
AU  - Braguglia CM
FAU - Rossetti, Simona
AU  - Rossetti S
LA  - eng
SI  - GENBANK/KF971872
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140828
PL  - England
TA  - FEMS Microbiol Lett
JT  - FEMS microbiology letters
JID - 7705721
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (Proteins)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
SB  - IM
MH  - DNA, Ribosomal/chemistry/genetics
MH  - Fermentation
MH  - Gram-Positive Bacteria/*classification/genetics/*isolation & purification
MH  - *In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - Oligonucleotide Probes/genetics
MH  - Proteins/*metabolism
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - Sewage/*microbiology
OTO - NOTNLM
OT  - Coprothermobacter
OT  - FISH
OT  - proteolytic bacteria
OT  - syntrophy
OT  - thermophilic anaerobic digestion
EDAT- 2014/07/22 06:00
MHDA- 2015/05/20 06:00
CRDT- 2014/07/22 06:00
PHST- 2014/03/07 00:00 [received]
PHST- 2014/06/29 00:00 [revised]
PHST- 2014/07/02 00:00 [accepted]
PHST- 2014/07/22 06:00 [entrez]
PHST- 2014/07/22 06:00 [pubmed]
PHST- 2015/05/20 06:00 [medline]
AID - 10.1111/1574-6968.12528 [doi]
PST - ppublish
SO  - FEMS Microbiol Lett. 2014 Sep;358(1):55-63. doi: 10.1111/1574-6968.12528. Epub 
      2014 Aug 28.