PMID- 25041985 OWN - NLM STAT- MEDLINE DCOM- 20150608 LR - 20181202 IS - 1872-7123 (Electronic) IS - 0165-1781 (Linking) VI - 219 IP - 3 DP - 2014 Nov 30 TI - Expression profile of neurotransmitter receptor and regulatory genes in the prefrontal cortex of spontaneously hypertensive rats: relevance to neuropsychiatric disorders. PG - 674-9 LID - S0165-1781(14)00405-3 [pii] LID - 10.1016/j.psychres.2014.05.034 [doi] AB - The spontaneously hypertensive rat (SHR) strain was shown to be a useful animal model to study several behavioral, pathophysiological and pharmacological aspects of schizophrenia and attention-deficit/hyperactivity disorder. To further understand the genetic underpinnings of this model, our primary goal in this study was to compare the gene expression profile of neurotransmitter receptors and regulators in the prefrontal cortex (PFC) and nucleus accumbens (NAcc) of SHR and Wistar rats (control group). In addition, we investigated DNA methylation pattern of promoter region of the genes differentially expressed. We performed gene expression analysis using a PCRarray technology, which simultaneously measures the expression of 84 genes related to neurotransmission. Four genes were significantly downregulated in the PFC of SHR compared to Wistar rats (Gad2, Chrnb4, Slc5a7, and Qrfpr) and none in nucleus accumbens. Gad2 and Qrfpr have CpG islands in their promoter region. For both, the promoter region was hypomethylated in SHR group, and probably this mechanism is not related with the downregulation of these genes. In summary, we identified genes that are downregulated in the PFC of SHR, and might be related to the behavioral abnormalities exhibited by this strain. CI - Copyright (c) 2014 Elsevier Ireland Ltd. All rights reserved. FAU - Santoro, Marcos Leite AU - Santoro ML AD - Genetics Division, Department of Morphology and Genetics, Federal University of Sao Paulo (UNIFESP), Sao Paulo, Brazil; Interdisciplinary Laboratory of Clinical Neurosciences (LiNC), Sao Paulo, Brazil. FAU - Santos, Camila Mauricio AU - Santos CM AD - Interdisciplinary Laboratory of Clinical Neurosciences (LiNC), Sao Paulo, Brazil; Department of Psychiatry, Federal University of Sao Paulo (UNIFESP), Sao Paulo, Brazil. FAU - Ota, Vanessa Kiyomi AU - Ota VK AD - Genetics Division, Department of Morphology and Genetics, Federal University of Sao Paulo (UNIFESP), Sao Paulo, Brazil; Interdisciplinary Laboratory of Clinical Neurosciences (LiNC), Sao Paulo, Brazil. FAU - Gadelha, Ary AU - Gadelha A AD - Interdisciplinary Laboratory of Clinical Neurosciences (LiNC), Sao Paulo, Brazil; Department of Psychiatry, Federal University of Sao Paulo (UNIFESP), Sao Paulo, Brazil. FAU - Stilhano, Roberta Sessa AU - Stilhano RS AD - Department of Biophysics and Investigation Center for Gene Therapy, Federal University of Sao Paulo (UNIFESP), Sao Paulo, Brazil. FAU - Diana, Mariana Cepollaro AU - Diana MC AD - Interdisciplinary Laboratory of Clinical Neurosciences (LiNC), Sao Paulo, Brazil; Department of Pharmacology, Federal University of Sao Paulo (UNIFESP), Sao Paulo, Brazil. FAU - Silva, Patricia Natalia AU - Silva PN AD - Genetics Division, Department of Morphology and Genetics, Federal University of Sao Paulo (UNIFESP), Sao Paulo, Brazil; Department of Psychiatry, Federal University of Sao Paulo (UNIFESP), Sao Paulo, Brazil. FAU - Spindola, Leticia Maria Nery AU - Spindola LM AD - Genetics Division, Department of Morphology and Genetics, Federal University of Sao Paulo (UNIFESP), Sao Paulo, Brazil. FAU - Melaragno, Maria Isabel AU - Melaragno MI AD - Genetics Division, Department of Morphology and Genetics, Federal University of Sao Paulo (UNIFESP), Sao Paulo, Brazil. FAU - Bressan, Rodrigo Affonseca AU - Bressan RA AD - Interdisciplinary Laboratory of Clinical Neurosciences (LiNC), Sao Paulo, Brazil; Department of Psychiatry, Federal University of Sao Paulo (UNIFESP), Sao Paulo, Brazil. FAU - Han, Sang Won AU - Han SW AD - Department of Biophysics and Investigation Center for Gene Therapy, Federal University of Sao Paulo (UNIFESP), Sao Paulo, Brazil. FAU - Abilio, Vanessa Costhek AU - Abilio VC AD - Interdisciplinary Laboratory of Clinical Neurosciences (LiNC), Sao Paulo, Brazil; Department of Pharmacology, Federal University of Sao Paulo (UNIFESP), Sao Paulo, Brazil. FAU - Belangero, Sintia Iole AU - Belangero SI AD - Genetics Division, Department of Morphology and Genetics, Federal University of Sao Paulo (UNIFESP), Sao Paulo, Brazil; Interdisciplinary Laboratory of Clinical Neurosciences (LiNC), Sao Paulo, Brazil; Department of Psychiatry, Federal University of Sao Paulo (UNIFESP), Sao Paulo, Brazil. Electronic address: sinbelangero@gmail.com. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20140605 PL - Ireland TA - Psychiatry Res JT - Psychiatry research JID - 7911385 RN - 0 (Chrnb4 protein, mouse) RN - 0 (Nerve Tissue Proteins) RN - 0 (Qrfpr protein, rat) RN - 0 (Receptors, G-Protein-Coupled) RN - 0 (Receptors, Neurotransmitter) RN - 0 (Receptors, Nicotinic) RN - 0 (SLC5A7 protein, human) RN - 0 (Symporters) RN - EC 4.1.1.15 (Glutamate Decarboxylase) RN - EC 4.1.1.15 (glutamate decarboxylase 2) SB - IM MH - Animals MH - Attention Deficit Disorder with Hyperactivity/*metabolism/*psychology MH - CpG Islands MH - Disease Models, Animal MH - Down-Regulation/genetics MH - Gene Expression MH - Glutamate Decarboxylase MH - Male MH - Nerve Tissue Proteins MH - Nucleus Accumbens/*metabolism/physiopathology MH - Polymerase Chain Reaction MH - Prefrontal Cortex/*metabolism/physiopathology MH - Rats MH - Rats, Inbred SHR MH - Rats, Inbred WKY MH - Rats, Wistar MH - Receptors, G-Protein-Coupled MH - Receptors, Neurotransmitter/*genetics MH - Receptors, Nicotinic MH - Symporters MH - *Synaptic Transmission OTO - NOTNLM OT - Chrnb4 OT - DNA methylation OT - Gad2 OT - Gene expression OT - Qrfpr OT - Slc5a7 OT - Spontaneously hypertensive rats EDAT- 2014/07/22 06:00 MHDA- 2015/06/09 06:00 CRDT- 2014/07/22 06:00 PHST- 2013/09/20 00:00 [received] PHST- 2014/04/22 00:00 [revised] PHST- 2014/05/18 00:00 [accepted] PHST- 2014/07/22 06:00 [entrez] PHST- 2014/07/22 06:00 [pubmed] PHST- 2015/06/09 06:00 [medline] AID - S0165-1781(14)00405-3 [pii] AID - 10.1016/j.psychres.2014.05.034 [doi] PST - ppublish SO - Psychiatry Res. 2014 Nov 30;219(3):674-9. doi: 10.1016/j.psychres.2014.05.034. Epub 2014 Jun 5.