PMID- 25043705
OWN - NLM
STAT- MEDLINE
DCOM- 20141205
LR  - 20181202
IS  - 1872-8227 (Electronic)
IS  - 0168-8227 (Linking)
VI  - 105
IP  - 3
DP  - 2014 Sep
TI  - One-week intervention period led to improvements in glycemic control and 
      reduction in DNA damage levels in patients with type 2 diabetes mellitus.
PG  - 356-63
LID - S0168-8227(14)00272-1 [pii]
LID - 10.1016/j.diabres.2014.06.004 [doi]
AB  - AIMS: Hyperglycemia leads to increased production of reactive oxygen species 
      (ROS), which reduces cellular antioxidant defenses and induces several DNA 
      lesions. We investigated the effects on DNA damage of a seven-day hospitalization 
      period in patients with type 2 diabetes mellitus (T2DM) to achieve adequate blood 
      glucose levels through dietary intervention and medication treatment, compared 
      with non-diabetic individuals. METHODS: DNA damage levels were evaluated by the 
      alkaline comet assay (with modified and without conventional use of hOGG1 enzyme, 
      which detects oxidized DNA bases) for 10 patients and 16 controls. Real time PCR 
      array method was performed to analyze the transcriptional expression of a set of 
      84 genes implicated in antioxidant defense and response to oxidative stress in 
      blood samples from T2DM patients (n=6) collected before and after the 
      hospitalization period. RESULTS: The seven-day period was sufficient to improve 
      glycemic control and to significantly decrease (p<0.05) DNA damage levels in T2DM 
      patients, although those levels were slightly higher than those in control 
      subjects. We also found a tendency towards a decrease in the levels of oxidative 
      DNA damage in T2DM patients after the hospitalization period. However, for all 
      genes analyzed, a statistically significant difference in the transcriptional 
      expression levels was not observed. CONCLUSIONS: The study demonstrated that 
      although the transcriptional expression of the genes studied did not show 
      significant alterations, one-week of glycemic control in hospital resulted in a 
      significant reduction in DNA damage levels detected in T2DM patients, 
      highlighting the importance of an adequate glycemic control.
CI  - Copyright (c) 2014 Elsevier Ireland Ltd. All rights reserved.
FAU - Xavier, Danilo J
AU  - Xavier DJ
AD  - Department of Genetics, Faculty of Medicine of Ribeirao Preto (FMRP), University 
      of Sao Paulo-USP, Ribeirao Preto, SP, Brazil.
FAU - Takahashi, Paula
AU  - Takahashi P
AD  - Department of Genetics, Faculty of Medicine of Ribeirao Preto (FMRP), University 
      of Sao Paulo-USP, Ribeirao Preto, SP, Brazil.
FAU - Manoel-Caetano, Fernanda S
AU  - Manoel-Caetano FS
AD  - Department of Genetics, Faculty of Medicine of Ribeirao Preto (FMRP), University 
      of Sao Paulo-USP, Ribeirao Preto, SP, Brazil; Department of Biology, Faculty of 
      Philosophy, Sciences and Letters of Ribeirao Preto (FFCLRP), University of Sao 
      Paulo-USP, Ribeirao Preto, SP, Brazil.
FAU - Foss-Freitas, Maria C
AU  - Foss-Freitas MC
AD  - Department of Internal Medicine, Faculty of Medicine of Ribeirao Preto, 
      University of Sao Paulo-USP, Ribeirao Preto, SP, Brazil.
FAU - Foss, Milton C
AU  - Foss MC
AD  - Department of Internal Medicine, Faculty of Medicine of Ribeirao Preto, 
      University of Sao Paulo-USP, Ribeirao Preto, SP, Brazil.
FAU - Donadi, Eduardo A
AU  - Donadi EA
AD  - Department of Genetics, Faculty of Medicine of Ribeirao Preto (FMRP), University 
      of Sao Paulo-USP, Ribeirao Preto, SP, Brazil; Division of Clinical Immunology, 
      Department of Medicine, Faculty of Medicine of Ribeirao Preto, University of Sao 
      Paulo-USP, Ribeirao Preto, SP, Brazil.
FAU - Passos, Geraldo A
AU  - Passos GA
AD  - Department of Genetics, Faculty of Medicine of Ribeirao Preto (FMRP), University 
      of Sao Paulo-USP, Ribeirao Preto, SP, Brazil; Disciplines of Genetics and 
      Molecular Biology, Department of Morphology, Faculty of Dentistry of Ribeirao 
      Preto, University of Sao Paulo-USP, Ribeirao Preto, SP, Brazil.
FAU - Sakamoto-Hojo, Elza T
AU  - Sakamoto-Hojo ET
AD  - Department of Genetics, Faculty of Medicine of Ribeirao Preto (FMRP), University 
      of Sao Paulo-USP, Ribeirao Preto, SP, Brazil; Department of Biology, Faculty of 
      Philosophy, Sciences and Letters of Ribeirao Preto (FFCLRP), University of Sao 
      Paulo-USP, Ribeirao Preto, SP, Brazil. Electronic address: etshojo@usp.br.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140621
PL  - Ireland
TA  - Diabetes Res Clin Pract
JT  - Diabetes research and clinical practice
JID - 8508335
RN  - 0 (Blood Glucose)
RN  - 0 (Reactive Oxygen Species)
SB  - IM
MH  - Adult
MH  - Blood Glucose/*analysis
MH  - *DNA Damage
MH  - Diabetes Mellitus, Type 2/*blood/*therapy
MH  - Female
MH  - Gene Expression Profiling
MH  - Hospitalization
MH  - Humans
MH  - Hyperglycemia/*therapy
MH  - Male
MH  - Middle Aged
MH  - Oxidative Stress/*genetics
MH  - Reactive Oxygen Species/blood
OTO - NOTNLM
OT  - Comet assay
OT  - Hyperglycemia
OT  - Oxidative stress
OT  - PCR array
OT  - Type 2 diabetes mellitus
EDAT- 2014/07/22 06:00
MHDA- 2014/12/15 06:00
CRDT- 2014/07/22 06:00
PHST- 2013/09/11 00:00 [received]
PHST- 2014/02/01 00:00 [revised]
PHST- 2014/06/13 00:00 [accepted]
PHST- 2014/07/22 06:00 [entrez]
PHST- 2014/07/22 06:00 [pubmed]
PHST- 2014/12/15 06:00 [medline]
AID - S0168-8227(14)00272-1 [pii]
AID - 10.1016/j.diabres.2014.06.004 [doi]
PST - ppublish
SO  - Diabetes Res Clin Pract. 2014 Sep;105(3):356-63. doi: 
      10.1016/j.diabres.2014.06.004. Epub 2014 Jun 21.