PMID- 25043728
OWN - NLM
STAT- MEDLINE
DCOM- 20150729
LR  - 20141202
IS  - 1618-1433 (Electronic)
IS  - 0940-2993 (Linking)
VI  - 66
IP  - 9-10
DP  - 2014 Dec
TI  - The protective effects of vitamin C on the DNA damage, antioxidant defenses and 
      aorta histopathology in chronic hyperhomocysteinemia induced rats.
PG  - 407-13
LID - S0940-2993(14)00089-X [pii]
LID - 10.1016/j.etp.2014.06.004 [doi]
AB  - The aim of this study was to investigate the protective effect of vitamin C 
      towards hyperhomocysteinemia (hHcy) induced oxidative DNA damage using the comet 
      assay. The increase in plasma homocysteine levels is an important risk factor for 
      vascular and cardiovascular diseases through free radical production. This study 
      was also conducted to investigate the histopathological changes in the thoracic 
      aorta and the oxidant/antioxidant status in heart, liver and kidney tissues. 
      Twenty-four adult male Wistar rats were divided as control, hHcy and hHcy+vitamin 
      C group. Chronic hHcy was induced by oral administration of l-methionine 
      (1g/kg/day) for 28 days. Vitamin C was given 150mg/kg/day within the specified 
      days. DNA damage was measured by use of the comet assay in lymphocytes. Levels of 
      malondialdehyde (MDA) and glutathione (GSH) as well as catalase (CAT) and 
      superoxide dismutase (SOD) activities were determined in heart, liver and renal 
      tissues. Results show that l-methionine administration significantly increased % 
      Tail DNA and Mean Tail Moment in hHcy group as compared with other groups. 
      Vitamin C treatment significantly decreased the high MDA levels and increased 
      activity of antioxidant enzymes in tissues. Aortic diameter and thickness of 
      aortic elastic laminae were significantly lower in hHcy+vitamin C group. Comet 
      assay can be used for the assessment of primary DNA damage caused by hHcy. 
      Histopathological findings showed that vitamin C may have a preventive effect in 
      alleviating the negative effects of hHcy. Vitamin C might be useful in the 
      prevention of endothelial dysfunction caused by hHcy.
CI  - Copyright (c) 2014 Elsevier GmbH. All rights reserved.
FAU - Boyacioglu, Murat
AU  - Boyacioglu M
AD  - Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Adnan 
      Menderes University, Isikli, Aydin, Turkey. Electronic address: 
      mboyacioglu@adu.edu.tr.
FAU - Sekkin, Selim
AU  - Sekkin S
AD  - Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Adnan 
      Menderes University, Isikli, Aydin, Turkey.
FAU - Kum, Cavit
AU  - Kum C
AD  - Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Adnan 
      Menderes University, Isikli, Aydin, Turkey.
FAU - Korkmaz, Deniz
AU  - Korkmaz D
AD  - Department of Histology and Embriology, Faculty of Veterinary Medicine, Adnan 
      Menderes University, Aydin, Turkey.
FAU - Kiral, Funda
AU  - Kiral F
AD  - Department of Biochemistry, Faculty of Veterinary Medicine, Adnan Menderes 
      University, Aydin, Turkey.
FAU - Yalinkilinc, Hande Sultan
AU  - Yalinkilinc HS
AD  - Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Adnan 
      Menderes University, Isikli, Aydin, Turkey.
FAU - Ak, Mehmet Onur
AU  - Ak MO
AD  - Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Adnan 
      Menderes University, Isikli, Aydin, Turkey.
FAU - Akar, Ferda
AU  - Akar F
AD  - Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Adnan 
      Menderes University, Isikli, Aydin, Turkey.
LA  - eng
PT  - Journal Article
DEP - 20140716
PL  - Germany
TA  - Exp Toxicol Pathol
JT  - Experimental and toxicologic pathology : official journal of the Gesellschaft fur 
      Toxikologische Pathologie
JID - 9208920
RN  - 0 (Antioxidants)
RN  - PQ6CK8PD0R (Ascorbic Acid)
SB  - IM
MH  - Animals
MH  - Antioxidants/*pharmacology
MH  - Aorta/*drug effects/pathology
MH  - Ascorbic Acid/*pharmacology
MH  - Comet Assay
MH  - *DNA Damage
MH  - Disease Models, Animal
MH  - Hyperhomocysteinemia/chemically induced/*pathology
MH  - Male
MH  - Oxidative Stress/drug effects
MH  - Rats
MH  - Rats, Wistar
OTO - NOTNLM
OT  - Comet assay
OT  - Hyperhomocysteinemia
OT  - Lymphocyt
OT  - Oxidative stress
OT  - Rats
OT  - Vitamin C
EDAT- 2014/07/22 06:00
MHDA- 2015/07/30 06:00
CRDT- 2014/07/22 06:00
PHST- 2014/01/20 00:00 [received]
PHST- 2014/05/07 00:00 [revised]
PHST- 2014/06/16 00:00 [accepted]
PHST- 2014/07/22 06:00 [entrez]
PHST- 2014/07/22 06:00 [pubmed]
PHST- 2015/07/30 06:00 [medline]
AID - S0940-2993(14)00089-X [pii]
AID - 10.1016/j.etp.2014.06.004 [doi]
PST - ppublish
SO  - Exp Toxicol Pathol. 2014 Dec;66(9-10):407-13. doi: 10.1016/j.etp.2014.06.004. 
      Epub 2014 Jul 16.