PMID- 25044338
OWN - NLM
STAT- MEDLINE
DCOM- 20151026
LR  - 20181202
IS  - 1552-4965 (Electronic)
IS  - 1549-3296 (Linking)
VI  - 103
IP  - 3
DP  - 2015 Mar
TI  - Poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) scaffolds coated with PhaP-RGD 
      fusion protein promotes the proliferation and chondrogenic differentiation of 
      human umbilical cord mesenchymal stem cells in vitro.
PG  - 1169-75
LID - 10.1002/jbm.a.35265 [doi]
AB  - Human umbilical cord blood-derived mesenchymal stem cells (hUC-MSCs) have been 
      widely used in tissue engineering. The aim of this study is to evaluate the 
      ability of poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) scaffolds 
      coated with polyhydroxyalkanoate binding protein fused with 
      arginyl-glycyl-aspartic acid (PhaP-RGD) to promote the proliferation and 
      chondrogenic differentiation of hUC-MSCs seeded on them. The PhaP-RGD fusion 
      protein was expressed by Escherichia coli. PHBHHx films were coated with PhaP-RGD 
      fusion protein and the physiochemical properties were examined. hUC-MSCs were 
      seeded on PHBHHx films with or without PhaP-RGD precoating and tested for changes 
      in morphology, viability, and chondrogenic differentiation. We found that 
      PhaP-RGD-coated PHBHHx films had similar surface morphology to uncoated PHBHHx. 
      The water contact angle of the coated PHBHHx surface was lower than that of the 
      uncoated surface (10.63 degrees  vs. 98.69 degrees ). At 7 and 14 days after seeding, the 
      PhaP-RGD-coated PHBHHx group showed greater numbers of viable cells compared to 
      the uncoated PHBHHx group. The expression levels of aggrecan and collagen II were 
      enhanced in the PhaP-RGD-coated PHBHHx group relative to the uncoated PHBHHx 
      group. Histological analysis using toluidine blue staining showed elevated 
      formation of proteoglycan producing chondrocytes in the PhaP-RGD-coated PHBHHx 
      group. Additionally, the synthesis of proteoglycan and collagen was significantly 
      enhanced within the PhaP-RGD constructs. Taken together, PhaP-RGD coating 
      promotes the proliferation and chondrogenic differentiation of hUC-MSCs seeded on 
      PHBHHx films. PhaP-RGD-coated PHBHHx may be a useful scaffold for cartilage 
      tissue engineering.
CI  - (c) 2014 Wiley Periodicals, Inc.
FAU - Li, Xiaoli
AU  - Li X
AD  - Department of Dermatology, The Second Hospital, Xi'an Jiaotong University, Xi'an, 
      China.
FAU - Chang, Huimin
AU  - Chang H
FAU - Luo, Huanan
AU  - Luo H
FAU - Wang, Zhenghui
AU  - Wang Z
FAU - Zheng, Guoxi
AU  - Zheng G
FAU - Lu, Xiaoyun
AU  - Lu X
FAU - He, Xijing
AU  - He X
FAU - Chen, Fang
AU  - Chen F
FAU - Wang, Ting
AU  - Wang T
FAU - Liang, Jianmin
AU  - Liang J
FAU - Xu, Min
AU  - Xu M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140718
PL  - United States
TA  - J Biomed Mater Res A
JT  - Journal of biomedical materials research. Part A
JID - 101234237
RN  - 0 (Bacterial Proteins)
RN  - 0 (Caproates)
RN  - 0 (Coated Materials, Biocompatible)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Oligopeptides)
RN  - 0 (PHAP protein, Bacteria)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (poly(3-hydroxybutyrate-co-3-hydroxyhexanoate))
RN  - 78VO7F77PN (arginyl-glycyl-aspartic acid)
RN  - TZP1275679 (3-Hydroxybutyric Acid)
SB  - IM
MH  - 3-Hydroxybutyric Acid/*chemistry
MH  - Bacterial Proteins/*chemistry
MH  - Caproates/*chemistry
MH  - *Cell Differentiation
MH  - *Cell Proliferation
MH  - Cells, Cultured
MH  - *Chondrogenesis
MH  - Coated Materials, Biocompatible/chemistry
MH  - DNA-Binding Proteins/*chemistry
MH  - Humans
MH  - Mesenchymal Stem Cells/cytology/*metabolism
MH  - Oligopeptides/*chemistry
MH  - Recombinant Fusion Proteins
MH  - Tissue Scaffolds/*chemistry
MH  - Umbilical Cord/cytology/*metabolism
OTO - NOTNLM
OT  - cartilage
OT  - polyhydroxyalkanoate
OT  - scaffold
OT  - surface modification
OT  - tissue engineering
EDAT- 2014/07/22 06:00
MHDA- 2015/10/27 06:00
CRDT- 2014/07/22 06:00
PHST- 2014/05/01 00:00 [received]
PHST- 2014/06/09 00:00 [revised]
PHST- 2014/06/13 00:00 [accepted]
PHST- 2014/07/22 06:00 [entrez]
PHST- 2014/07/22 06:00 [pubmed]
PHST- 2015/10/27 06:00 [medline]
AID - 10.1002/jbm.a.35265 [doi]
PST - ppublish
SO  - J Biomed Mater Res A. 2015 Mar;103(3):1169-75. doi: 10.1002/jbm.a.35265. Epub 
      2014 Jul 18.