PMID- 25044720
OWN - NLM
STAT- MEDLINE
DCOM- 20160404
LR  - 20140820
IS  - 1552-4930 (Electronic)
IS  - 1552-4922 (Linking)
VI  - 85
IP  - 9
DP  - 2014 Sep
TI  - Analysis of centromere signal patterns in breast cancer cells with chromosomal 
      instability using image cytometry combined with centromere fluorescence in situ 
      hybridization.
PG  - 809-16
LID - 10.1002/cyto.a.22502 [doi]
AB  - Fluorescence in situ hybridization (FISH) with centromeric probes is a method 
      used to detect chromosomal instability (CIN), a hallmark of most cancers. 
      However, no studies thus far have investigated the relationship between 
      centromeric FISH signals and the cell cycle in cancer cells. In this study, the 
      chromosome content in each cell cycle phase was evaluated with respect to the 
      number of centromeric FISH signals in two breast cancer cell lines and eight 
      surgically resected breast cancer specimens using image cytometry. Variations in 
      chromosome number were detected at each phase of the cell cycle but were not 
      associated with proliferative capacity in the cell lines. Furthermore, the 
      chromosome doubling frequency differed in each cell line and clinical specimen. 
      These results reveal two aspects of centromeric FISH signal variation in breast 
      cancers that exhibit CIN, and suggest that chromosome doubling is a remarkable 
      occurrence that may increase the heterogeneity of tumors.
CI  - (c) 2014 International Society for Advancement of Cytometry.
FAU - Ito, Hideaki
AU  - Ito H
AD  - Department of Pathology, Yamaguchi University Graduate School of Medicine, Ube, 
      Japan.
FAU - Oga, Atsunori
AU  - Oga A
FAU - Ikemoto, Kenzo
AU  - Ikemoto K
FAU - Furuya, Tomoko
AU  - Furuya T
FAU - Maeda, Noriko
AU  - Maeda N
FAU - Yamamoto, Shigeru
AU  - Yamamoto S
FAU - Kawauchi, Shigeto
AU  - Kawauchi S
FAU - Itoh, Hiroshi
AU  - Itoh H
FAU - Oka, Masaaki
AU  - Oka M
FAU - Sasaki, Kohsuke
AU  - Sasaki K
LA  - eng
PT  - Journal Article
DEP - 20140709
PL  - United States
TA  - Cytometry A
JT  - Cytometry. Part A : the journal of the International Society for Analytical 
      Cytology
JID - 101235694
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - *Aneuploidy
MH  - Breast Neoplasms/*genetics
MH  - Cell Cycle/genetics
MH  - Cell Line, Tumor
MH  - Centromere/genetics
MH  - Chromosomal Instability/*genetics
MH  - DNA, Neoplasm/analysis/*genetics
MH  - Female
MH  - Humans
MH  - Image Cytometry
MH  - In Situ Hybridization, Fluorescence
MH  - MCF-7 Cells
MH  - Signal Transduction/genetics
OTO - NOTNLM
OT  - aneuploidy
OT  - cell cycle
OT  - chromosomal instability
OT  - fluorescence in situ hybridization
OT  - image cytometry
EDAT- 2014/07/22 06:00
MHDA- 2016/04/05 06:00
CRDT- 2014/07/22 06:00
PHST- 2014/03/05 00:00 [received]
PHST- 2014/06/05 00:00 [revised]
PHST- 2014/06/12 00:00 [accepted]
PHST- 2014/07/22 06:00 [entrez]
PHST- 2014/07/22 06:00 [pubmed]
PHST- 2016/04/05 06:00 [medline]
AID - 10.1002/cyto.a.22502 [doi]
PST - ppublish
SO  - Cytometry A. 2014 Sep;85(9):809-16. doi: 10.1002/cyto.a.22502. Epub 2014 Jul 9.