PMID- 25045014
OWN - NLM
STAT- MEDLINE
DCOM- 20150126
LR  - 20151119
IS  - 1934-6638 (Electronic)
IS  - 1934-662X (Linking)
VI  - 122
IP  - 11
DP  - 2014 Nov
TI  - A panel of protein markers for the early detection of lung cancer with bronchial 
      brushing specimens.
PG  - 833-41
LID - 10.1002/cncy.21461 [doi]
AB  - BACKGROUND: To date, no robust biomarkers have been available in clinical 
      practice that can provide an early diagnostic evaluation of lung cancer. The 
      objective of this study was to identify potential biomarkers for the early 
      detection of lung cancer using bronchial brushing specimens. METHODS: 
      Immunocytochemistry was used to investigate the expression of 35 proteins in 880 
      bronchial brushing specimens from both outpatients and inpatients who had either 
      lung cancer or benign lung lesions. An optimal panel was identified that had high 
      sensitivity and considerable specificity for detecting lung cancer. Associations 
      between protein expression and clinicopathologic parameters were assessed. 
      RESULTS: Tumor protein 53 (TP53), TP63, Ki67, epidermal growth factor receptor 
      (EGFR), minichromosome maintenance complex component 6 (MCM6), MCM7, 
      uncharacterized proteins KIAA1522 and KIAA0317, and ubiquitin-protein ligase UHR1 
      (ICBP90) frequently presented high expression in bronchial brushing specimens 
      from patients who had lung cancer compared with patients who had benign lung 
      lesions. A 6-protein panel consisting of TP53, Ki67, MCM6, MCM7, KIAA1522, and 
      KIAA0317 was identified as the best combination, with sensitivity of 81.1% (309 
      of 381 specimens) for detecting nonsmall cell lung cancer (NSCLC) and 86.8% (145 
      of 167 specimens) for detecting small cell lung cancer (SCLC) (specificity, 
      83.3%; 65 of 78 specimens). The combination of cytology and the protein panel 
      significantly improved the sensitivity of bronchial brushing examination for 
      detecting lung cancer (P<.00001), which increased from 49.1% to 81% in early 
      stage NSCLC (stage I and II). In combined analyses, the protein panel was 
      positively associated with patient sex (P=.00033), tumor type (P<.00001), tumor 
      location (P<.00001), and lymph node metastasis (P=.028). CONCLUSIONS: The 
      6-protein panel is a potential biomarker for the early detection of lung cancer 
      in bronchial brushings.
CI  - (c) 2014 American Cancer Society.
FAU - Liu, Yi-Zhen
AU  - Liu YZ
AD  - State Key Laboratory of Molecular Oncology, Cancer Institute/Hospital, Peking 
      Union Medical College and Chinese Academy of Medical Sciences, Beijing, China.
FAU - Jiang, Yan-Yi
AU  - Jiang YY
FAU - Wang, Bo-Shi
AU  - Wang BS
FAU - Hao, Jia-Jie
AU  - Hao JJ
FAU - Shang, Li
AU  - Shang L
FAU - Zhang, Tong-Tong
AU  - Zhang TT
FAU - Cao, Jian
AU  - Cao J
FAU - Xu, Xin
AU  - Xu X
FAU - Zhan, Qi-Min
AU  - Zhan QM
FAU - Wang, Ming-Rong
AU  - Wang MR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140709
PL  - United States
TA  - Cancer Cytopathol
JT  - Cancer cytopathology
JID - 101499453
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Proto-Oncogene Proteins)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biomarkers, Tumor/*biosynthesis
MH  - Bronchi/pathology
MH  - Bronchoscopy
MH  - Carcinoma, Non-Small-Cell Lung/diagnosis/metabolism
MH  - Cytodiagnosis/methods
MH  - Diagnosis, Differential
MH  - Early Diagnosis
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Lung Neoplasms/*diagnosis/*metabolism
MH  - Male
MH  - Middle Aged
MH  - Proto-Oncogene Proteins/*biosynthesis
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Small Cell Lung Carcinoma/diagnosis/metabolism
MH  - Specimen Handling/methods
MH  - Young Adult
OTO - NOTNLM
OT  - biomarker
OT  - bronchial brushings
OT  - nonsmall cell lung cancer
OT  - protein panel
OT  - small cell lung cancer
EDAT- 2014/07/22 06:00
MHDA- 2015/01/27 06:00
CRDT- 2014/07/22 06:00
PHST- 2014/04/24 00:00 [received]
PHST- 2014/05/29 00:00 [revised]
PHST- 2014/06/10 00:00 [accepted]
PHST- 2014/07/22 06:00 [entrez]
PHST- 2014/07/22 06:00 [pubmed]
PHST- 2015/01/27 06:00 [medline]
AID - 10.1002/cncy.21461 [doi]
PST - ppublish
SO  - Cancer Cytopathol. 2014 Nov;122(11):833-41. doi: 10.1002/cncy.21461. Epub 2014 
      Jul 9.