PMID- 25045659 OWN - NLM STAT- MEDLINE DCOM- 20150312 LR - 20211021 IS - 2314-6141 (Electronic) IS - 2314-6133 (Print) VI - 2014 DP - 2014 TI - Multipotent neural crest stem cell-like cells from rat vibrissa dermal papilla induce neuronal differentiation of PC12 cells. PG - 186239 LID - 10.1155/2014/186239 [doi] LID - 186239 AB - Bone marrow mesenchymal stem cells (BMSCs) transplants have been approved for treating central nervous system (CNS) injuries and diseases; however, their clinical applications are limited. Here, we model the therapeutic potential of dermal papilla cells (DPCs) in vitro. DPCs were isolated from rat vibrissae and characterized by immunocytofluorescence, RT-PCR, and multidifferentiation assays. We examined whether these cells could secrete neurotrophic factors (NTFs) by using cocultures of rat pheochromocytoma cells (PC12) with conditioned medium and ELISA assay. DPCs expressed Sox10, P75, Nestin, Sox9, and differentiated into adipocytes, osteoblasts, smooth muscle cells, and neurons under specific inducing conditions. The DPC-conditioned medium (DPC-CM) induced neuronal differentiation of PC12 cells and promoted neurite outgrowth. Results of ELISA assay showed that compared to BMSCs, DPCs secreted more brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF). Moreover, we observed that, compared with the total DPC population, sphere-forming DPCs expressed higher levels of Nestin and P75 and secreted greater amounts of GDNF. The DPCs from craniofacial hair follicle papilla may be a new and promising source for treating CNS injuries and diseases. FAU - Li, Meiying AU - Li M AUID- ORCID: 0000-0003-4802-5859 AD - The Key Laboratory of Pathobiology, Ministry of Education, Jilin University, 126 Xinmin Street, Changchun, Jilin 130021, China. FAU - Liu, Jin Yu AU - Liu JY AUID- ORCID: 0000-0002-1000-9434 AD - The Key Laboratory of Pathobiology, Ministry of Education, Jilin University, 126 Xinmin Street, Changchun, Jilin 130021, China. FAU - Wang, Shichao AU - Wang S AUID- ORCID: 0000-0002-4111-6283 AD - Department of Gynaecology, Third Hospital of Jilin University, Changchun, China. FAU - Xu, Hao AU - Xu H AD - Department of Biological Engineering, College of Pharmacy, Jilin University, Changchun, China. FAU - Cui, Lifeng AU - Cui L AUID- ORCID: 0000-0003-0755-8333 AD - Department of Gynaecology, First Hospital of Jilin University, Changchun, China. FAU - Lv, Shuang AU - Lv S AUID- ORCID: 0000-0002-2741-0291 AD - The Key Laboratory of Pathobiology, Ministry of Education, Jilin University, 126 Xinmin Street, Changchun, Jilin 130021, China. FAU - Xu, Jinying AU - Xu J AD - The Key Laboratory of Pathobiology, Ministry of Education, Jilin University, 126 Xinmin Street, Changchun, Jilin 130021, China. FAU - Liu, Shutong AU - Liu S AD - The Key Laboratory of Pathobiology, Ministry of Education, Jilin University, 126 Xinmin Street, Changchun, Jilin 130021, China. FAU - Chi, Guangfan AU - Chi G AUID- ORCID: 0000-0001-7096-9106 AD - The Key Laboratory of Pathobiology, Ministry of Education, Jilin University, 126 Xinmin Street, Changchun, Jilin 130021, China. FAU - Li, Yulin AU - Li Y AUID- ORCID: 0000-0002-3758-7568 AD - The Key Laboratory of Pathobiology, Ministry of Education, Jilin University, 126 Xinmin Street, Changchun, Jilin 130021, China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20140618 PL - United States TA - Biomed Res Int JT - BioMed research international JID - 101600173 SB - IM MH - Animals MH - Cell Differentiation/*genetics MH - Coculture Techniques MH - Hair Follicle/cytology MH - Multipotent Stem Cells/*cytology MH - Neural Crest/cytology MH - Neurons/*cytology MH - Organ of Corti/cytology/growth & development MH - PC12 Cells MH - Rats MH - Vibrissae/*cytology/growth & development PMC - PMC4086521 EDAT- 2014/07/22 06:00 MHDA- 2015/03/13 06:00 PMCR- 2014/06/18 CRDT- 2014/07/22 06:00 PHST- 2014/01/28 00:00 [received] PHST- 2014/04/24 00:00 [accepted] PHST- 2014/07/22 06:00 [entrez] PHST- 2014/07/22 06:00 [pubmed] PHST- 2015/03/13 06:00 [medline] PHST- 2014/06/18 00:00 [pmc-release] AID - 10.1155/2014/186239 [doi] PST - ppublish SO - Biomed Res Int. 2014;2014:186239. doi: 10.1155/2014/186239. Epub 2014 Jun 18.