PMID- 25046861
OWN - NLM
STAT- MEDLINE
DCOM- 20150715
LR  - 20170830
IS  - 1096-0937 (Electronic)
IS  - 1087-1845 (Linking)
VI  - 72
DP  - 2014 Nov
TI  - The effect of Pleurotus ostreatus arabinofuranosidase and its evolved variant in 
      lignocellulosic biomasses conversion.
PG  - 162-167
LID - S1087-1845(14)00118-2 [pii]
LID - 10.1016/j.fgb.2014.07.003 [doi]
AB  - The fungal arabinofuranosidase from Pleurotus ostreatus PoAbf recombinantly 
      expressed in Pichia pastoris rPoAbf and its evolved variant rPoAbf F435Y/Y446F 
      were tested for their effectiveness to enhance the enzymatic saccharification of 
      three lignocellulosic biomasses, namely Arundo donax, corn cobs and brewer's 
      spent grains (BSG), after chemical or chemical-physical pretreatment. All the raw 
      materials were subjected to an alkaline pretreatment by soaking in aqueous 
      ammonia solution whilst the biomass from A. donax was also pretreated by steam 
      explosion. The capability of the wild-type and mutant rPoAbf to increase the 
      fermentable sugars recovery was assessed by using these enzymes in combination 
      with different (hemi)cellulolytic activities. These enzymatic mixtures were 
      either entirely of commercial origin or contained the cellulase from Streptomyces 
      sp. G12 CelStrep recombinantly expressed in Escherichia coli in substitution to 
      the commercial counterparts. The addition of the arabinofuranosidases from P. 
      ostreatus improved the hydrolytic efficiency of the commercial enzymatic 
      cocktails on all the pretreated biomasses. The best results were obtained using 
      the rPoAbf evolved variant and are represented by increases of the xylose 
      recovery up to 56.4%. These data clearly highlight the important role of the 
      accessory hemicellulolytic activities to optimize the xylan bioconversion yields.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Marcolongo, Loredana
AU  - Marcolongo L
AD  - Institute of Biosciences and BioResources, National Research Council, Napoli, 
      Italy.
FAU - Ionata, Elena
AU  - Ionata E
AD  - Institute of Biosciences and BioResources, National Research Council, Napoli, 
      Italy.
FAU - La Cara, Francesco
AU  - La Cara F
AD  - Institute of Biosciences and BioResources, National Research Council, Napoli, 
      Italy.
FAU - Amore, Antonella
AU  - Amore A
AD  - Department of Chemical Sciences, University of Naples "Federico II", Napoli, 
      Italy.
FAU - Giacobbe, Simona
AU  - Giacobbe S
AD  - Department of Chemical Sciences, University of Naples "Federico II", Napoli, 
      Italy.
FAU - Pepe, Olimpia
AU  - Pepe O
AD  - Department of Agriculture, University of Naples "Federico II", Portici (Napoli), 
      Italy.
FAU - Faraco, Vincenza
AU  - Faraco V
AD  - Department of Chemical Sciences, University of Naples "Federico II", Napoli, 
      Italy. Electronic address: vfaraco@unina.it.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140719
PL  - United States
TA  - Fungal Genet Biol
JT  - Fungal genetics and biology : FG & B
JID - 9607601
RN  - 11132-73-3 (lignocellulose)
RN  - 9005-53-2 (Lignin)
RN  - EC 3.2.1.- (Glycoside Hydrolases)
RN  - EC 3.2.1.55 (alpha-N-arabinofuranosidase)
SB  - IM
MH  - Biomass
MH  - Cloning, Molecular
MH  - Escherichia coli/enzymology/genetics
MH  - Gene Expression
MH  - Glycoside Hydrolases/genetics/*metabolism
MH  - Hydrolysis
MH  - Lignin/*metabolism
MH  - Pichia/enzymology/genetics
MH  - Pleurotus/*enzymology/genetics/metabolism
MH  - Streptomyces/enzymology/genetics
OTO - NOTNLM
OT  - Alkaline pretreatment
OT  - Arabinofuranosidase
OT  - Lignocellulose
OT  - Pleurotus ostreatus
OT  - Saccharification
OT  - Steam explosion
EDAT- 2014/07/22 06:00
MHDA- 2015/07/16 06:00
CRDT- 2014/07/22 06:00
PHST- 2014/03/29 00:00 [received]
PHST- 2014/07/03 00:00 [revised]
PHST- 2014/07/07 00:00 [accepted]
PHST- 2014/07/22 06:00 [entrez]
PHST- 2014/07/22 06:00 [pubmed]
PHST- 2015/07/16 06:00 [medline]
AID - S1087-1845(14)00118-2 [pii]
AID - 10.1016/j.fgb.2014.07.003 [doi]
PST - ppublish
SO  - Fungal Genet Biol. 2014 Nov;72:162-167. doi: 10.1016/j.fgb.2014.07.003. Epub 2014 
      Jul 19.