PMID- 25047013
OWN - NLM
STAT- MEDLINE
DCOM- 20150824
LR  - 20181202
IS  - 1930-739X (Electronic)
IS  - 1930-7381 (Linking)
VI  - 22
IP  - 11
DP  - 2014 Nov
TI  - Identification of mechanisms of action of bisphenol a-induced human preadipocyte 
      differentiation by transcriptional profiling.
PG  - 2333-43
LID - 10.1002/oby.20848 [doi]
AB  - OBJECTIVE: Exposure to the endocrine-disrupting chemical bisphenol A (BPA) is 
      correlated with obesity and adipogenesis of human preadipocytes. However, the 
      mechanism of action of BPA-induced human adipogenesis remains to be determined. 
      METHODS: Primary human preadipocytes were differentiated in the presence of 50 microM 
      BPA or 1 microM dexamethasone (DEX) for 48 hours. Potential mechanisms of BPA-induced 
      adipogenesis were evaluated using gene expression microarray analysis. RESULTS: 
      Microarray analysis revealed 373 differentially expressed genes following BPA 
      treatment, including upregulation of sterol regulatory element binding factor 1 
      (SREBF1), a key regulator of lipid metabolism. For DEX-treated preadipocytes, 
      2167 genes were differentially expressed, including upregulation of the 
      adipogenic marker lipoprotein lipase. Ingenuity Pathway Analysis was used to 
      identify functional annotations of the gene expression changes associated with 
      response to BPA and DEX. BPA exposure was associated with expression changes in 
      the genes involved in triacylglycerol accumulation while DEX was linked to 
      triacylglycerol and fatty acid metabolism. The analysis also revealed enrichment 
      of genes following BPA exposure in the thyroid-receptor/retinoic X receptor 
      (TR/RXR) and mammalian target of rapamycin (mTOR) signaling pathways. 
      CONCLUSIONS: Our data suggest that potential mechanisms of action of BPA-induced 
      adipogenesis involve SREBF1, the TR/RXR, and the mTOR pathways.
CI  - (c) 2014 The Obesity Society.
FAU - Boucher, Jonathan G
AU  - Boucher JG
AD  - In Vitro Molecular Toxicology Laboratory, Environmental Health Science and 
      Research Bureau, Health Canada, Ottawa, Canada.
FAU - Husain, Mainul
AU  - Husain M
FAU - Rowan-Carroll, Andrea
AU  - Rowan-Carroll A
FAU - Williams, Andrew
AU  - Williams A
FAU - Yauk, Carole L
AU  - Yauk CL
FAU - Atlas, Ella
AU  - Atlas E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140722
PL  - United States
TA  - Obesity (Silver Spring)
JT  - Obesity (Silver Spring, Md.)
JID - 101264860
RN  - 0 (Benzhydryl Compounds)
RN  - 0 (Phenols)
RN  - 0 (Triglycerides)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - EC 3.1.1.34 (Lipoprotein Lipase)
RN  - MLT3645I99 (bisphenol A)
SB  - IM
MH  - Adipocytes/*drug effects/physiology
MH  - Adipogenesis/drug effects/genetics
MH  - Benzhydryl Compounds/*pharmacology
MH  - Cell Differentiation/*drug effects/genetics
MH  - Cells, Cultured
MH  - Dexamethasone/pharmacology
MH  - *Gene Expression Profiling
MH  - Gene Expression Regulation/*drug effects
MH  - Humans
MH  - Lipid Metabolism/genetics
MH  - Lipoprotein Lipase/metabolism
MH  - Microarray Analysis
MH  - Obesity/genetics/metabolism/pathology
MH  - Phenols/*pharmacology
MH  - Signal Transduction/genetics
MH  - Triglycerides/metabolism
EDAT- 2014/07/23 06:00
MHDA- 2015/08/25 06:00
CRDT- 2014/07/23 06:00
PHST- 2014/04/28 00:00 [received]
PHST- 2014/07/09 00:00 [accepted]
PHST- 2014/07/23 06:00 [entrez]
PHST- 2014/07/23 06:00 [pubmed]
PHST- 2015/08/25 06:00 [medline]
AID - 10.1002/oby.20848 [doi]
PST - ppublish
SO  - Obesity (Silver Spring). 2014 Nov;22(11):2333-43. doi: 10.1002/oby.20848. Epub 
      2014 Jul 22.