PMID- 2504711
OWN - NLM
STAT- MEDLINE
DCOM- 19891011
LR  - 20210210
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 264
IP  - 25
DP  - 1989 Sep 5
TI  - Microinjection of a 19-kDa guanine nucleotide-binding protein inhibits maturation 
      of Xenopus oocytes.
PG  - 14824-8
AB  - ADP-ribosylation factors (ARFs) are 19-21-kDa proteins purified from bovine brain 
      that bind guanosine 5'-triphosphate (GTP). They exhibit GTP-dependent activity as 
      activators of cholera toxin-catalyzed ADP-ribosylation of the alpha-subunit of 
      the stimulatory guanine nucleotide-binding protein of the adenylyl cyclase system 
      (Gs alpha). ARF, which interacts directly with the catalytic subunit of cholera 
      toxin, has no known physiologic role. Intracellular microinjection of ARF was 
      employed to investigate the effect of ARF on progesterone- and insulin-stimulated 
      maturation of Xenopus oocytes. Maturation was inhibited by injection of ARF 3-8 h 
      before exposure of oocytes to progesterone or insulin. ARF inhibition was 
      dependent on progesterone concentration but not on insulin concentration. 
      Inhibition was enhanced by concomitant injection of GTP and to a greater extent 
      by guanosine 5'-O-(thiotriphosphate) (GTP gamma S) which, in the absence of ARF, 
      inhibited somewhat at early time points. The demonstration of this effect of ARF 
      on both progesterone- and insulin-stimulated oocyte maturation may provide a clue 
      to the physiologic role of this guanine nucleotide-binding protein.
FAU - Bahnson, T D
AU  - Bahnson TD
AD  - Laboratory of Cellular Metabolism, National Heart, Lung, and Blood Institute, 
      Bethesda, Maryland 20892.
FAU - Tsai, S C
AU  - Tsai SC
FAU - Adamik, R
AU  - Adamik R
FAU - Moss, J
AU  - Moss J
FAU - Vaughan, M
AU  - Vaughan M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Growth Inhibitors)
RN  - 0 (Insulin)
RN  - 0 (Membrane Proteins)
RN  - 146-91-8 (Guanosine Diphosphate)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - EC 3.6.5.2 (ADP-Ribosylation Factors)
SB  - IM
MH  - ADP-Ribosylation Factors
MH  - Animals
MH  - Female
MH  - GTP-Binding Proteins/analysis/*pharmacology
MH  - Growth Inhibitors/*pharmacology
MH  - Guanosine Diphosphate/pharmacology
MH  - Guanosine Triphosphate/pharmacology
MH  - Insulin/pharmacology
MH  - Membrane Proteins/analysis/*pharmacology
MH  - Microinjections
MH  - Molecular Weight
MH  - Oocytes/drug effects/*growth & development/physiology
MH  - Oogenesis/*drug effects
MH  - Progesterone/pharmacology
MH  - Time Factors
MH  - Xenopus laevis
EDAT- 1989/09/05 00:00
MHDA- 1989/09/05 00:01
CRDT- 1989/09/05 00:00
PHST- 1989/09/05 00:00 [pubmed]
PHST- 1989/09/05 00:01 [medline]
PHST- 1989/09/05 00:00 [entrez]
AID - S0021-9258(18)63774-3 [pii]
PST - ppublish
SO  - J Biol Chem. 1989 Sep 5;264(25):14824-8.