PMID- 25047905
OWN - NLM
STAT- MEDLINE
DCOM- 20150702
LR  - 20211203
IS  - 1347-7439 (Electronic)
IS  - 0916-7250 (Print)
IS  - 0916-7250 (Linking)
VI  - 76
IP  - 10
DP  - 2014 Oct
TI  - Application of a SYBR(R)Green one step real-time RT-PCR assay to detect type 1 
      porcine reproductive and respiratory syndrome virus.
PG  - 1411-3
AB  - The emergence in Japan of field isolates of type 1 porcine reproductive and 
      respiratory syndrome virus (PRRSV) suggests problems with control. We therefore 
      developed a one-step real-time reverse transcription polymerase chain reaction 
      (qRT-PCR) with improved sensitivity that detects as little as 1 x 10(-2) 
      TCID50/ml of viral RNA. We tested serum samples collected in January and 
      September 2008, October 2009 and January 2011 from a farm with an outbreak and 
      found infected pigs between January and September 2008, but not in January 2011. 
      Further, between 2008 and 2011, we did not detect infection in pigs at 8 nearby 
      farms or in 2,052 serum samples collected from pigs from 74 farms in 12 
      prefectures. This assay should help prevent future outbreaks.
FAU - Iseki, Hiroshi
AU  - Iseki H
AD  - Viral Disease and Epidemiological Research Division, National Institute of Animal 
      Health, NARO, Tsukuba, Ibaraki 305-0856, Japan.
FAU - Takagi, Michihiro
AU  - Takagi M
FAU - Kuroda, Yoshiko
AU  - Kuroda Y
FAU - Katsuda, Ken
AU  - Katsuda K
FAU - Mikami, Osamu
AU  - Mikami O
FAU - Tsunemitsu, Hiroshi
AU  - Tsunemitsu H
FAU - Yamakawa, Makoto
AU  - Yamakawa M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140718
PL  - Japan
TA  - J Vet Med Sci
JT  - The Journal of veterinary medical science
JID - 9105360
RN  - 0 (Benzothiazoles)
RN  - 0 (Diamines)
RN  - 0 (Organic Chemicals)
RN  - 0 (Quinolines)
RN  - 163795-75-3 (SYBR Green I)
SB  - IM
MH  - Animals
MH  - Benzothiazoles
MH  - Diamines
MH  - Japan/epidemiology
MH  - Organic Chemicals
MH  - Porcine Reproductive and Respiratory Syndrome/diagnosis/epidemiology/*virology
MH  - Porcine respiratory and reproductive syndrome virus/classification/*isolation & 
      purification
MH  - Quinolines
MH  - Real-Time Polymerase Chain Reaction/methods/*veterinary
MH  - Reverse Transcriptase Polymerase Chain Reaction/methods/*veterinary
MH  - Swine
MH  - Time Factors
PMC - PMC4221178
EDAT- 2014/07/23 06:00
MHDA- 2015/07/03 06:00
PMCR- 2014/10/01
CRDT- 2014/07/23 06:00
PHST- 2014/07/23 06:00 [entrez]
PHST- 2014/07/23 06:00 [pubmed]
PHST- 2015/07/03 06:00 [medline]
PHST- 2014/10/01 00:00 [pmc-release]
AID - DN/JST.JSTAGE/jvms/14-0010 [pii]
AID - 14-0010 [pii]
AID - 10.1292/jvms.14-0010 [doi]
PST - ppublish
SO  - J Vet Med Sci. 2014 Oct;76(10):1411-3. doi: 10.1292/jvms.14-0010. Epub 2014 Jul 
      18.